PPBI Nodes


PPBI is organized as a consortium of top research universities and institutes in Portugal, consisting of 20 core facilities distributed across three regional poles: Douro & Minho, Mondego & Beiras, and Tejo & Algarve. These nodes offer a comprehensive range of services in bioimaging, including access to imaging equipment, wet lab and sample preparation tools, computational clusters for data storage and analysis, as well as image analysis and training through courses, workshops, and online resources.

Additionally, our infrastructure supports animal facilities for both model and non-model organisms, providing services such as equipment operation, consulting, project planning, technology development, and outreach activities. Our highly qualified PPBI staff is dedicated to supporting all these activities and services, ensuring the success of your research projects.

PPBI ORGANIZATION POLES/CLUSTERS: 

North Imaging Cluster (NIC)

i3S* (IBMC, INEB, IPATIMUP) and FMUP in Porto, ICVS* and INL (observer node) in Braga

Center Imaging Cluster (CIC)

CNC-UC* and iLAB-UC* in Coimbra, iBiMED* in Aveiro and CICS-UBI* in Covilhã;

South Imaging Cluster (SIC)

 CF, FCUL*, iBB-IST*, ITQB-NOVA*, GIMM*, NMS* in Lisbon and ABC-RI-UAlg/CCMAR* in Algarve.

Nodes

Explore the nodes
(click the image)

Nodes
Braga Porto Aveiro Coimbra Covilhã Lisbon Algarve ICVS-UM INL i3S iBiMED-UA CNC-UC iLAB UC CICS-UBI ITQB-NOVA GIMM (Oeiras) Champalimaud Foundation - CF NMS FCUL iBB-IST GIMM (Lisbon) ABC-RI-UAlg

Algarve

ICVS-UM

ICVS-UM

Offered Technologies

  • Deconvolution widefield microscopy (DWM)
  • Laser scanning confocal microscopy (LSCM/CLSM)
  • Two-photon microscopy (2P)
  • Fluorescence Resonance Energy Transfer (FRET)
  • Fluorescence Recovery After Photobleaching (FRAP)
  • Fluorescence Lifetime Imaging Microscopy (FLIM)

INL

INL

Offered Technologies

  • Transmission Electron Microscopy (TEM)
  • Scanning Electron Microscopy (SEM)

i3S

i3S

Offered Technologies

  • Deconvolution widefield microscopy (DWM)
  • Laser scanning confocal microscopy (LSCM/CLSM)
  • Spinning disk confocal microscopy (SDCM)
  • Structured illumination microscopy (SIM)
  • Two-photon microscopy (2P)
  • Total internal reflection fluorescence microscopy (TIRF)
  • Single Molecule localization microscopy (SMLM)
  • Stimulated emission depletion microscopy (STED)
  • Light-sheet mesoscopic imaging (SPIM or dSLSM)
  • Raman Spectroscopy (RS)
  • Second/Third Harmonics Generation (SHG/THG)
  • High throughput microscopy/high content screening (HTM/HCS)
  • Fluorescence (cross)-correlation spectroscopy (FCS/FCCS)
  • Fluorescence Resonance Energy Transfer (FRET)
  • Fluorescence Recovery After Photobleaching (FRAP)
  • Fluorescence Lifetime Imaging Microscopy (FLIM)
  • Intravital Microscopy (IVM)
  • Photomanipulation
  • Anisotropy/Polarization Microscopy
  • Expansion Microscopy
  • Tissue Clearing (TC)
  • Single molecule FRET (smFRET)
  • FTIR Spectroscopy
  • Imaging Flow Cytometry
  • microcomputed tomography (microCT)
  • Conventional ultrastructure
  • Immunoelectromicroscopy
  • Energy Dispersive X-Ray Spectrometry (EDS)
  • Atomic Force Microscopy

iBiMED-UA

iBiMED-UA

Offered Technologies

  • Deconvolution widefield microscopy (DWM)
  • Laser scanning confocal microscopy (LSCM/CLSM)
  • Spinning disk confocal microscopy (SDCM)
  • Fluorescence Resonance Energy Transfer (FRET)
  • Fluorescence Recovery After Photobleaching (FRAP)
  • Imaging Flow Cytometry

CNC-UC

CNC-UC

Offered Technologies

  • Deconvolution widefield microscopy (DWM)
  • Laser scanning confocal microscopy (LSCM/CLSM)
  • Spinning disk confocal microscopy (SDCM)
  • Two-photon microscopy (2P)
  • Fluorescence Resonance Energy Transfer (FRET)
  • Fluorescence Recovery After Photobleaching (FRAP)
  • Intravital Microscopy (IVM)
  • Microdissection
  • Tissue Clearing (TC)

iLAB UC

iLAB-UC

Offered Technologies

  • Deconvolution widefield microscopy (DWM)
  • Laser scanning confocal microscopy (LSCM/CLSM)
  • Transmission Electron Microscopy (TEM)
  • Scanning Electron Microscopy (SEM)

CICS-UBI

CICS-UBI

Offered Technologies

  • Deconvolution widefield microscopy (DWM)
  • Laser scanning confocal microscopy (LSCM/CLSM)
  • Fluorescence Resonance Energy Transfer (FRET)
  • Fluorescence Recovery After Photobleaching (FRAP)

ITQB-NOVA

ITQB-NOVA

Offered Technologies

  • Deconvolution widefield microscopy (DWM)
  • Laser scanning confocal microscopy (LSCM/CLSM)
  • Structured illumination microscopy (SIM)
  • Total internal reflection fluorescence microscopy (TIRF)
  • Single Molecule localization microscopy (SMLM)
  • Fluorescence Resonance Energy Transfer (FRET)
  • Fluorescence Recovery After Photobleaching (FRAP)
  • Fluorescence Lifetime Imaging Microscopy (FLIM)
  • Imaging at Biosafety Level >1
  • Expansion Microscopy

GIMM (Oeiras)

GIMM (Oeiras)

Offered Technologies

  • Deconvolution widefield microscopy (DWM)
  • Laser scanning confocal microscopy (LSCM/CLSM)
  • Spinning disk confocal microscopy (SDCM)
  • Structured illumination microscopy (SIM)
  • Two-photon microscopy (2P)
  • Total internal reflection fluorescence microscopy (TIRF)
  • Single Molecule localization microscopy (SMLM)
  • Light-sheet mesoscopic imaging (SPIM or dSLSM)
  • Optical projection tomography (OPT)
  • Second/Third Harmonics Generation (SHG/THG)
  • High throughput microscopy/high content screening (HTM/HCS)
  • Fluorescence Resonance Energy Transfer (FRET)
  • Fluorescence Recovery After Photobleaching (FRAP)
  • Intravital Microscopy (IVM)
  • Microdissection
  • Imaging at Biosafety Level >1
  • Photomanipulation
  • Expansion Microscopy
  • Tissue Clearing (TC)
  • Transmission Electron Microscopy (TEM)
  • Electron Tomography

Champalimaud Foundation – CF

Champalimaud Foundation

Offered Technologies

  • Deconvolution widefield microscopy (DWM)
  • Laser scanning confocal microscopy (LSCM/CLSM)
  • Light-sheet mesoscopic imaging (SPIM or dSLSM)
  • High throughput microscopy/high content screening (HTM/HCS)
  • Fluorescence Recovery After Photobleaching (FRAP)

NMS

NMS

Offered Technologies

  • Deconvolution widefield microscopy (DWM)
  • Laser scanning confocal microscopy (LSCM/CLSM)
  • Spinning disk confocal microscopy (SDCM)

FCUL

FCUL

Offered Technologies

  • Deconvolution widefield microscopy (DWM)
  • Laser scanning confocal microscopy (LSCM/CLSM)
  • High throughput microscopy/high content screening (HTM/HCS)
  • Fluorescence Lifetime Imaging Microscopy (FLIM)
  • Transmission Electron Microscopy (TEM)
  • Scanning Electron Microscopy (SEM)

iBB-IST

iBB-IST

Offered Technologies

  • Laser scanning confocal microscopy (LSCM/CLSM)
  • Two-photon microscopy (2P)
  • Fluorescence (cross)-correlation spectroscopy (FCS/FCCS)
  • Fluorescence Resonance Energy Transfer (FRET)
  • Fluorescence Recovery After Photobleaching (FRAP)
  • Fluorescence Lifetime Imaging Microscopy (FLIM)
  • Anisotropy/Polarization Microscopy
  • Single molecule FRET (smFRET)

GIMM (Lisbon)

GIMM (Lisbon)

Offered Technologies

  • Deconvolution widefield microscopy (DWM)
  • Laser scanning confocal microscopy (LSCM/CLSM)
  • Spinning disk confocal microscopy (SDCM)
  • Two-photon microscopy (2P)
  • Second/Third Harmonics Generation (SHG/THG)
  • High throughput microscopy/high content screening (HTM/HCS)
  • Fluorescence Resonance Energy Transfer (FRET)
  • Fluorescence Recovery After Photobleaching (FRAP)
  • Intravital Microscopy (IVM)
  • Photomanipulation
  • Expansion Microscopy
  • Tissue Clearing (TC)
  • Imaging Flow Cytometry

ABC-RI-UAlg

ABC-RI-UAlg

Offered Technologies

  • Deconvolution widefield microscopy (DWM)
  • Laser scanning confocal microscopy (LSCM/CLSM)
  • Spinning disk confocal microscopy (SDCM)
  • Fluorescence Resonance Energy Transfer (FRET)
  • Fluorescence Recovery After Photobleaching (FRAP)

 * Nodes that also belong to EuroBioImaging PPBI Node

PPBI  consortium institutions:

Instituto de Biologia Molecular e Celular (IBMC/i3S); Instituto Nacional de Engenharia Biomédica (INEB/i3S); Instituto de Patologia e Imunologia Molecular (IPATIMUP/i3S); Faculdade de Medicina da Universidade do Porto (FMUP); Universidade do Minho (UM); Centro de Neurociências e Biologia Celular (CNC); Universidade de Coimbra (UC); Universidade de Aveiro (UA); Universidade da Beira Interior (UBI); Faculdade de Ciências Médicas (FCM/UNL); Instituto de Tecnologia Química e Biológica (NOVA-ITQB/UNL); Instituto Gulbenkian de Medicina Molecular (GIMM); Faculdade de Ciências da Universidade de Lisboa (FCUL); Instituto Superior Técnico (IST/UL); Fundação D. Anna de Sommer Champalimaud e Dr. Carlos Montez Champalimaud (FC); Universidade do Algarve (UAlg).