Advanced Fluorescence Microscopy Facility – iBB-IST
The Advanced Fluorescence Microscopy Facility at the Institute for Bioengineering and Biosciences (iBB) is specialized in quantitative imaging to describe the organization, dynamics and molecular interactions in both in vitro and cellular systems. The facility stands out in the application of cutting-edge fluorescence microscopy techniques (including at the single-molecule level) and in the development of quantitative modeling.
The team attained international recognition in molecular and cell biophysics. Remarkably, we implemented for the first time – Fluorescence anisotropy imaging microscopy (FAIM)and single-molecule FRET – in Portugal. The unit also provides scientific support in project development, technical training, and guidance in image acquisition/ analysis.
Resources
Equipment:
- Olympus IX83
- Home-built confocal microscope for single-molecule FRET
- Leica SP5 Multiphoton
Software:
- MatLab
Offered Technologies
- Laser Scanning Confocal Microscopy (LSCM/CLSM)
- Multiphoton Microscopy
- Fluorescence (Cross)-Correlation Spectroscopy (FCS/FCCS)
- Förster Resonance Energy Transfer (FRET)
- Fluorescence Recovery After Photobleaching (FRAP)
- Fluorescence Lifetime Imaging Microscopy (FLIM)
- Anisotropy/Polarization Microscopy
- Single Molecule FRET (smFRET)
Publications
- Fabio Fernandes, T. Tuyen Dao, Manuel Prieto. Hybrid polymer/lipid vesicles: Insights on phase separation and dynamics from time-resolved fluorescence and microscopy. Biophysical Journal, Volume 122, Issue 3, Supplement 1, 2023, Page 225a.
- Salazar SB, Pinheiro MJF, Sotti-Novais D, Soares AR, Lopes MM, Ferreira T, Rodrigues V, Fernandes F, Mira NP. Disclosing azole resistance mechanisms in resistant Candida glabrata strains encoding wild-type or gain-of-function CgPDR1 alleles through comparative genomics and transcriptomics. G3 (Bethesda). 2022 Jul 6;12(7):jkac110. doi: 10.1093/g3journal/jkac110. PMID: 35532173; PMCID: PMC9258547.
- Russo R, Padanha R, Fernandes F, Veiros LF, Corzana F, Gois PMP. Engineering Boron Hot Spots for the Site-Selective Installation of Iminoboronates on Peptide Chains. Chemistry. 2020 Nov 26;26(66):15226-15231. doi: 10.1002/chem.202002675. Epub 2020 Oct 19. PMID: 32627856.
- Dias TP, Baltazar T, Pinto SN, Fernandes TG, Fernandes F, Diogo MM, Prieto M, Cabral JMS. Xeno-Free Integrated Platform for Robust Production of Cardiomyocyte Sheets from hiPSCs. Stem Cells Int. 2022 Nov 23;2022:4542719. doi: 10.1155/2022/4542719. PMID: 36467280; PMCID: PMC9712013.
- Dias TP, Pinto SN, Carvalho S, Fernandes TG, Fernandes F, Diogo MM, Peleteiro MC, Prieto M, Cabral JMS. Cost-Effective Mechanical Aggregation of Cardiac Progenitors and Encapsulation in Matrigel Support Self-Organization in a Dynamic Culture Environment. Int J Mol Sci. 2022 Dec 13;23(24):15785. doi: 10.3390/ijms232415785. PMID: 36555427; PMCID: PMC9779514.
- Borges-Araújo L, Monteiro ME, Mil-Homens D, Bernardes N, Sarmento MJ, Coutinho A, Prieto M, Fernandes F. Impact of Ca2+-Induced PI(4,5)P2 Clusters on PH-YFP Organization and Protein-Protein Interactions. Biomolecules. 2022 Jun 29;12(7):912. doi: 10.3390/biom12070912. PMID: 35883468; PMCID: PMC9312469.
- Sarmento MJ, Borges-Araújo L, Pinto SN, Bernardes N, Ricardo JC, Coutinho A, Prieto M, Fernandes F. Quantitative FRET Microscopy Reveals a Crucial Role of Cytoskeleton in Promoting PI(4,5)P2 Confinement. Int J Mol Sci. 2021 Oct 29;22(21):11727. doi: 10.3390/ijms222111727. PMID: 34769158; PMCID: PMC8583820.
- Ribeiro RA, Vitorino MV, Godinho CP, Bourbon-Melo N, Robalo TT, Fernandes F, Rodrigues MS, Sá-Correia I. Yeast adaptive response to acetic acid stress involves structural alterations and increased stiffness of the cell wall. Sci Rep. 2021 Jun 16;11(1):12652. doi: 10.1038/s41598-021-92069-3. PMID: 34135398; PMCID: PMC8209030.
- Garizo AR, Castro F, Martins C, Almeida A, Dias TP, Fernardes F, Barrias CC, Bernardes N, Fialho AM, Sarmento B. p28-functionalized PLGA nanoparticles loaded with gefitinib reduce tumor burden and metastases formation on lung cancer. J Control Release. 2021 Sep 10;337:329-342. doi: 10.1016/j.jconrel.2021.07.035. Epub 2021 Jul 24. PMID: 34311024.
- Garizo AR, Coelho LF, Pinto S, Dias TP, Fernandes F, Bernardes N, Fialho AM. The Azurin-Derived Peptide CT-p19LC Exhibits Membrane-Active Properties and Induces Cancer Cell Death. Biomedicines. 2021 Sep 10;9(9):1194. doi: 10.3390/biomedicines9091194. PMID: 34572379; PMCID: PMC8469032.
- Pinto SN, Dias SA, Cruz AF, Mil-Homens D, Fernandes F, Valle J, Andreu D, Prieto M, Castanho MARB, Coutinho A, Veiga AS. The mechanism of action of pepR, a viral-derived peptide, against Staphylococcus aureus biofilms. J Antimicrob Chemother. 2019 Sep 1;74(9):2617-2625. doi: 10.1093/jac/dkz223. PMID: 31127270; PMCID: PMC6736180.
- Reichmann NT, Tavares AC, Saraiva BM, Jousselin A, Reed P, Pereira AR, Monteiro JM, Sobral RG, VanNieuwenhze MS, Fernandes F, Pinho MG. SEDS-bPBP pairs direct lateral and septal peptidoglycan synthesis in Staphylococcus aureus. Nat Microbiol. 2019 Aug;4(8):1368-1377. doi: 10.1038/s41564-019-0437-2. Epub 2019 May 13. PMID: 31086309.
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