ABBE Platform
The ABBE Platform provides the equipment and expertise to support Champalimaud Foundation researchers in the acquisition of high-quality microscopy data. The platform assists users throughout the complete imaging pipeline, including project discussions to identify the most appropriate imaging strategy, detailed technical training and support in image processing, analysis and visualisation.
The ABBE platform is managed by an international team with multidisciplinary background, from Bio-Phisics to Physics and brings together expertise from light-sheet microscopy to super-resolution imaging. The team also represents the Champalimaud Foundation on an international level through conferences and committees and serves as bridge between CF and microscopy companies.
Resources
Equipment:
- ZEISS LSM 980 with Airyscan 2
- ZEISS LSM 880 with Airyscan
- ZEISS AxioImager M2
- ZEISS AxioScan Z1
- ZEISS AxioScope A1 (DIC)
- ZEISS Lumar V12
- ZEISS Discovery V8
- ZEISS Lightsheet Z.1
- Miltenyi UltraMicroscope Blaze
Software:
- Imaris
- ZEISS arivis
- Huygens
- MatLab
- ZEISS ZEN
- QuPath
- Fiji
Offered Technologies
- Deconvolution Widefield Microscopy (DWM)
- Laser Scanning Confocal Microscopy (LSCM/CLSM)
- Light-sheet Mesoscopic Imaging (SPIM or dSLSM)
- High Throughput Microscopy/High Content Sreening (HTM/HCS)
- Fluorescence Recovery After Photobleaching (FRAP)
- High Performance Workstation
- High Performance Workstation with high end GPU
- High Performance Computational Cluster
Publications
- Susanne Bour, Yanina Dening, Melanie Balbach, Ina Poser, Inés Ramírez Álvarez, Helena den Haan, Christoph Kluge, Ronald Naumann, Reinhard Oertel, Irene Alba-Alejandre, Davide Accardi, Christian G. Stief, Marianne Dieterich, Peter Falkai, Rainer A. Böckmann, Horacio Pérez-Sánchez, Anthony A. Hyman, Matthias Trottmann, Francisco Pan-Montojo. A conserved role of Parkinson-associated DJ-1 metabolites in sperm motility, mitosis, and embryonic development. bioRxiv 2021.01.16.426934.
- Madan E, Pelham CJ, Nagane M, Parker TM, Canas-Marques R, Fazio K, Shaik K, Yuan Y, Henriques V, Galzerano A, Yamashita T, Pinto MAF, Palma AM, Camacho D, Vieira A, Soldini D, Nakshatri H, Post SR, Rhiner C, Yamashita H, Accardi D, Hansen LA, Carvalho C, Beltran AL, Kuppusamy P, Gogna R, Moreno E. Flower isoforms promote competitive growth in cancer. Nature. 2019 Aug;572(7768):260-264. doi: 10.1038/s41586-019-1429-3. Epub 2019 Jul 24. PMID: 31341286.
- Darmohray DM, Jacobs JR, Marques HG, Carey MR. Spatial and Temporal Locomotor Learning in Mouse Cerebellum. Neuron. 2019 Apr 3;102(1):217-231.e4. doi: 10.1016/j.neuron.2019.01.038. Epub 2019 Feb 19. PMID: 30795901.
- Tomás Cruz, Terufumi Fujiwara, Nélia Varela, Farhan Mohammad, Adam Claridge-Chang, M Eugenia Chiappe. Motor context coordinates visually guided walking in Drosophila. bioRxiv 572792
- F.F. Esteves, D. Matias, A.R. Mendes, B. Lacoste, S.Q. Lima. Sexually dimorphic neuronal inputs to the neuroendocrine dopaminergic system governing prolactin release. J. Neuroendocrinol., 31 (2019), Article e12781.
- Matias S, Lottem E, Dugué GP, Mainen ZF. Activity patterns of serotonin neurons underlying cognitive flexibility. Elife. 2017 Mar 21;6:e20552. doi: 10.7554/eLife.20552. PMID: 28322190; PMCID: PMC5360447.
- A new thalamo-cortical-amygdala circuit is involved in processing a natural auditory alarm cue. A new thalamo-cortical-amygdala circuit is involved in processing a natural auditory alarm cue. PLOS Biology, 18(5), e3000674 – May 2020
- Steck K, Walker SJ, Itskov PM, Baltazar C, Moreira JM, Ribeiro C. Internal amino acid state modulates yeast taste neurons to support protein homeostasis in Drosophila. Elife. 2018 Feb 2;7:e31625. doi: 10.7554/eLife.31625. PMID: 29393045; PMCID: PMC5812714.
Job Shadowing
- Specialised training in light-sheet microscopy
- Spectral Unmixing
- Airyscan I and II + multiplex
- Training for quality control in terms of beads preparation for PSF measurements and how to standardize LPM