INL Imaging Platforms
The Research Core Facilities of INL are a centralised infrastructure that provides access to advanced equipment, techniques, and expertise.
It operates as an open-access facility and offers researchers from academia and the industrial sector a portfolio of services ranging from design to fabrication and characterisation.
Nanophotonics & Bioimaging
Advanced Electron Microscopy
Nanophotonics & Bioimaging
The Nanophotonics & Bioimaging (NBI) facility features cutting-edge optical instrumentation and the know-how required for the characterization of material and life sciences samples.
The INL expert team provides knowledge in the analysis of light-matter interactions at various scales: reaching from the detection of individual fluorescent molecules or nanoparticles to the high-resolution label-free and fluorescence-based imaging of fixed and live cells, e.g. in 2D cell models or tissues.
Furthermore, we are experienced in designing optically active nanomaterials and characterising e.g. plasmonic interaction effects or surface-enhanced Raman effects (SERS) and studying optical properties of metamaterials.
Operating as an open access facility, we provide support in training and service measurements on spectroscopy and imaging equipment, as well as complementary services, such as specimen/sample preparation, flow cytometry, and spectral or image analysis. Since 2020, the NBI facility is integrated in the Portuguese Platform of Bioimaging (PPBI).
Resources
Equipment:
- ZEISS LSM 880 with Airyscan
Software:
Offered Technologies
- Laser Scanning Confocal Microscopy (LSCM/CLSM)
- Widefield Microscopy
- Image Analysis Software
Publications
Job Shadowing
Advanced Electron Microscopy Facility
This facility focuses on materials and biological research, the development of novel techniques and instrumentation, as well as providing training, technical support and consultation in the areas of electron microscopy and spectroscopy. The centre facility houses several electron microscopes that can probe the physical, electronic, biological, and chemical structure of matter down to the atomic scale.
These instruments are coupled with advanced in-situ holders, in which the environment is controlled to match near-realistic conditions of operation, and the sample’s behaviour is recorded dynamically in real time. State-of-the-art support facilities also are available, including standard specimen preparation equipment and an image analysis laboratory.
Resources
Equipment:
- FEI Helios NanoLab 450S Dual Beam – FIB with UHREM FEG-SEM
- JEOL JEM 2100 80-200kV
- Probe-Corrected FEI Titan G2 80-200 kV ChemiSTEM
- Double-Corrected FEI Titan G3 Cubed Themis 60-300 kV
- Automated Critical Point Dryer Leica EM CPD300
Software:
Offered Technologies
- Scanning Electron Microscopy (SEM)
- Image Analysis Software
- Transmission Electron Microscopy (TEM)
- Sample preparation
Publications
Job Shadowing