

NMS Microscopy Facility
The NMS Research microscopy facility possess the scientific know-how and technical knowledge in advanced microscopy and image analysis to support and enhance scientific research. We also provide access to a wide variety of instruments covering from basic to advanced light microscopy, available to all research community (NMS and External users).
The Facility staff assists researchers in the experimental planning of light microscopy experiments, provides in-depth training for the operation of the microscopes as well as troubleshooting during microscopes operation.
Additionally, support is provided in the processing, rendering and analysis of acquired datasets. If needed, custom analysis routines will be provided as a service.
For the continued high-quality service, it is our mission to keep our resources updated and our staff on top of the latest microscopy developments and techniques.
Resources
Equipment:
- ZEISS AxioImager Z2
- ZEISS Cell Observer SD
- ZEISS LSM 980 with Airyscan 2
- ZEISS LSM 710
- ZEISS Axio Observer Z1
Software:
- Huygens
- Imaris
- ZEISS ZEN
- CellProfiler
- Fiji
- MatLab
- ilastik
Offered Technologies
- Spinning disk confocal microscopy (SDCM)
- Deconvolution Widefield Microscopy (DWM)
- Laser Scanning Confocal Microscopy (LSCM/CLSM)
- Fluorescence Recovery After Photobleaching (FRAP)
- Förster Resonance Energy Transfer (FRET)
- High Performance Workstation
Publications
- Ferreira JV, da Rosa Soares A, Pereira P. LAMP2A mediates the loading of proteins into endosomes and selects exosomal cargo. Autophagy. 2022 Jun 27;:1-3. doi: 10.1080/15548627.2022.2092315. [Epub ahead of print] PubMed PMID: 35761480.
- Ana S Brandão , Jorge Borbinha, Telmo Pereira, Patrícia H Brito, Raquel Lourenço, Anabela Bensimon-Brito, Antonio Jacinto (2022) A regeneration-triggered metabolic adaptation is necessary for cell identity transitions and cell cycle re-entry to support blastema formation and bone regeneration eLife 11:e76987. https://doi.org/10.7554/eLife.76987
Job Shadowing
- Facility Management
- Image Analysis and Processing
- User Training