FCUL Microscopy Facility

The mission of FCUL Microscopy Facility (FCUL-MF) is to provide open access to instrumentation, expert support and training to answer scientific questions using bioimaging techniques such as stereo, widefield fluorescence, confocal and electron microscopy. We support our users in assay development, acquisition of bioimaging data, image quantification and data analysis.

The Facility has a key specialization in High-Throughput Screening (HTS), supporting image-based drug discovery and functional genomics projects. A strong emphasis is placed on the efficient managing of siRNA and small molecule libraries to identify biologically relevant hit compounds through automated workflows.

By acting as a hub for bioimaging technologies, training, service provision and support, the Facility aims to foster scientific excellence and have positive societal impacts.

Visit Node Website

Team

Rui Malhó

Coordinator

Hugo Botelho

Co-coordinator

Luís Marques

Microscopy Technician

Gabriela Rodrigues

Coordination Comission

Sólveig Thorsteinsdóttir

Coordination Comission

Telmo Nunes

Microscopy Technician

Resources

Equipment:

Leica TCS SP8
Leica TCS SPE
Leica TCS SP5
Leica DMI6000B
ZEISS Axiovert 200
Olympus IX50
Olympus BX51
Olympus BX60
ZEISS SteREO LUMAR V.12

Software:

Fiji
Imaris
CellProfiler
CellProfiler Analyst
ilastik
AMIRA
LAS X
Metaphase Professional
R
shinyHTM

Offered Technologies

Deconvolution Widefield Microscopy (DWM)
High Throughput Microscopy/High Content Sreening (HTM/HCS)
Laser Scanning Confocal Microscopy (LSCM/CLSM)
Automated Point Scanning Confocal Microscopy
Cryosectioning
Fluorescence Lifetime Imaging Microscopy (FLIM)
Förster Resonance Energy Transfer (FRET)
Fluorescence Recovery After Photobleaching (FRAP)
Fluorescence Stereo Microscopes
Image Analysis Software
Imaging at Biosafety Level >1

Publications

Teixeira RT, Marchese D, Duckney PJ, Dias FV, Carapeto AP, Louro M, Silva MS, Cordeiro C, Rodrigues MS, Malhó R (2024) Functional characterization reveals the importance of Arabidopsis ECA4 and EPSIN3 in clathrin mediated endocytosis and wall structure in apical growing cells. New Phytol, 245(3):1056-1071. DOI: 1111/nph.20282
Martins SG, Ribeiro V, Melo C, Paulino-Cavaco C, Antonini D, Naidu SD, Murtinheira F, Fonseca I, Saget B, Pita M, Fernandes DR, dos Santos PG, Rodrigues G, Zilhão R, Herrera F, Dinkova-Kostova AT, Carlos AR, Thorsteinsdóttir S (2024) Laminin-α2 chain deficiency in skeletal muscle causes dysregulation of multiple cellular mechanisms. Life Sci Alliance, 7(12):e202402829. DOI: 26508/lsa.202402829
Bierlaagh MC, Ramalho AS, Silva IAL, Vonk AM, van den Bor RM, van Mourik P, Pott J, Suen SWF, Boj SF, Vries RGJ, Lammertyn E, Vermeulen F, Amaral MD, de Boeck K, van der Ent CK, Beekman JM (2024) Repeatability and reproducibility of the Forskolin-induced swelling (FIS) assay on intestinal organoids from people with Cystic Fibrosis. J Cyst Fibros, 23(4):693-702. DOI: 1016/j.jcf.2024.04.014
Guerreiro DR, Saraiva JG, Borges MJ, Sampaio JM, Peralta L (2024) Development of a plastic scintillating optical fibers array dosimeter for radiobiology. J Instrum, 19:P05006. DOI: 1088/1748-0221/19/05/P05006
Ferreira FC, Amaral MD, Bacalhau M, Pacheco ML (2024) PTI-801 (posenacaftor) shares a common mechanism with VX-445 (elexacaftor) to rescue p.Phe508del-CFTR. Eur J Pharmacol, 967:176390. DOI: 1016/j.ejphar.2024.176390
Diallo M, Pimenta C, Murtinheira F, Martins-Alves D, Pinto FR, da Costa AA, Letra-Vilela R, Martin V, Rodriguez C, Rodrigues MS, Herrera F (2024) Asymmetric post-translational modifications regulate the nuclear translocation of STAT3 homodimers in response to leukemia inhibitory factor. Cell Oncol, 47:1065-1070. DOI: 1007/s13402-023-00911-9
Caetano S, Garcia AR, Figueira I, Brito MA (2023) MEF2C and miR-194-5p: New Players in Triple Negative Breast Cancer Tumorigenesis. Int J Mol Sci, 24(18):14297. DOI: 3390/ijms241814297
Ferreira-Peralta P, França B, Murtinheira F, Rodrigues MS, Herrera F (2024) Is spastic ataxia 8 a protein misfolding disorder?. Biochim Biophys Acta Mol Basis Dis, 1870(1):166882. DOI: 1016/j.bbadis.2023.166882
Godinho-Pereira J, Vaz D, Figueira I, Aniceto-Romão J, Krizbai I, Malhó M, Rocha J, Carvalheiro MC, Simões S, Gaspar MM, Brito MA (2023) Breast Cancer Brain Metastases: Implementation and Characterization of a Mouse Model Relying on Malignant Cells Inoculation in the Carotid Artery. Cells, 12(16):2076. DOI: 3390/cells12162076
Garcia AR, Mendes A, Custódia C, Faria CC, Barata JT, Malhó R, Figueira I, Brito MA (2023) Abrogating Metastatic Properties of Triple-Negative Breast Cancer Cells by EGFR and PI3K Dual Inhibitors. Cancers, 15(15):3973. DOI: 3390/cancers15153973
Fernandes BF, Silva N, Marques JF, Da Cruz MB, Tiainen L, Gasik M, Carvalho O, Silva FS, Caramês J, Mata A (2023) Bio-Piezoelectric Ceramic Composites for Electroactive Implants-Biological Performance. Biomimetics, 8(4):338. DOI: 3390/biomimetics8040338
Leitão MIPS, Turos-Cabal M, Sanchez-Sanchez AM, Gomes C, Fernandez VM, Herrera F, Petronilho A (2023) Antitumor Activity and Reductive Stress by Platinum(II) N-Heterocyclic Carbenes based on Guanosine. Chemistry, 29(40):e202301078. DOI: 1002/chem.202301078
Railean V, Rodrigues CS, Ramalho SS, Silva IAL, Bartosch J, Farinha CM, Pankonien I, Amaral MD (2023) Personalized medicine: Function of CFTR variant p.Arg334Trp is rescued by currently available CFTR modulators. Front Mol Biosci, 10:1155705. DOI: 3389/fmolb.2023.1155705
dos Santos PG, Soares AR, Thorsteinsdóttir S, Rodrigues G (2023) Preparation of 3D Decellularized Matrices from Fetal Mouse Skeletal Muscle for Cell Culture. J Vis Exp, (193):. DOI: 3791/65069
Bacalhau M, Ferreira FC, Silva IAL, Buarque CD, Amaral MD, Lopes-Pacheco M (2023) Additive Potentiation of R334W-CFTR Function by Novel Small Molecules. J Pers Med, 13(1):102. DOI: 3390/jpm13010102
Sebastiana M, Serrazina S, Monteiro F, Wipf D, Fromentin J, Teixeira R, Malhó R, Courty P-E (2023) Nitrogen Acquisition and Transport in the Ectomycorrhizal Symbiosis—Insights from the Interaction between an Oak Tree and Pisolithus tinctorius. Plants, 12(1):10. DOI: 3390/plants12010010
Moreira GG, Gomes CM (2023) Tau liquid-liquid phase separation is modulated by the Ca2+ -switched chaperone activity of the S100B protein. J Neurochem, 166(1):76-86. DOI: 1111/jnc.15756
Bacalhau M, Ferreira FC, Kmit, A, Souza FR, da Silva VD, Pimentel AS, Amaral MD, Buarque CD, Lopes-Pacheco M (2023) Identification of novel F508del-CFTR traffic correctors among triazole derivatives. Eur J Pharmacol, 938:175396. DOI: 1016/j.ejphar.2022.175396
Teixeira A, Noronha H, Sebastiana M, Fortes AM, Gerós H (2022) A proteomic analysis shows the stimulation of light reactions and inhibition of the Calvin cycle in the skin chloroplasts of ripe red grape berries. Front Plant Sci, 13:1014532. DOI: 3389/fpls.2022.1014532
Amaro R, Diniz I, Santos H, Pimentel D, Rego C, Mithöfer A, Fortes AM (2023) Hormone Changes in Tolerant and Susceptible Grapevine Leaves Under Powdery Mildew Infection. J Plant Growth Regul, 42:3606-3614. DOI: 1007/s00344-022-10823-x
Estorninho M, Chozas S, Mendes A, Colwell F, Abrantes I, Fonseca L, Fernandes P, Costa C, Máguas C, Correia O, Antunes C (2022) Differential Impact of the Pinewood Nematode on Pinus Species Under Drought Conditions. Front Plant Sci, 13:841707. DOI: 3389/fpls.2022.841707
Rodenburg LW, Delpiano L, Railean V, Centeio R, Pinto MC, Smits SMA, van der Windt IS, van Hugten CFJ, van Beuningen SFB, Rodenburg RNP, van der Ent CK, Amaral MD, Kunzelmann K, Gray MA, Beekman JM, Amatngalim GD (2022) Drug Repurposing for Cystic Fibrosis: Identification of Drugs That Induce CFTR-Independent Fluid Secretion in Nasal Organoids. Int J Mol Sci, 23(20):12657. DOI: 3390/ijms232012657
Encarnação S, de Mello-Sampayo C, Carrapiço B, Braz BS, Jordão AP, Peleteiro C, Catarino L, da Silva IBM, Gouveia LF, Lima BS, Silva O (2022) Anacardium occidentale Bark as an Antidiabetic Agent. Plants, 11(19):2637. DOI: 3390/plants11192637
Godinho-Pereira J, Lopes MD, Garcia AR, Botelho HM, Malhó R, Figueira I, Brito MA (2022) A Drug Screening Reveals Minocycline Hydrochloride as a Therapeutic Option to Prevent Breast Cancer Cells Extravasation across the Blood–Brain Barrier. Biomedicines, 10(8):1988. DOI: 3390/biomedicines10081988
Ferreira JF, Silva IAL, Botelho HM, Amaral MD, Farinha CM (2022) Absence of EPAC1 Signaling to Stabilize CFTR in Intestinal Organoids. Cells, 11(15):2295. DOI: 10.3390/cells11152295
Franco F, Kausar S, Silva MFB, Guedes RC, Falcao AO, Brito MA (2022) Multi-Targeting Approach in Glioblastoma Using Computer-Assisted Drug Discovery Tools to Overcome the Blood–Brain Barrier and Target EGFR/PI3Kp110β Signaling. Cancers, 14(4):3506. DOI: 3390/cancers14143506
Fernandes C, Palma E, Silva F, Belchior A, Pinto CIG, Guerreiro FJ, Botelho HM, Mendes F, Raposinho P, Paulo A (2022) Searching for a Paradigm Shift in Auger-Electron Cancer Therapy with Tumor-Specific Radiopeptides Targeting the Mitochondria and/or the Cell Nucleus. Int J Mol Sci, 23(13):7238. DOI: 3390/ijms23137238
de Almeida PG, Rifes P, Martins-Jesus AP, Pinheiro GG, Andrade RP, Thorsteinsdóttir S (2022) Cell–Fibronectin Interactions and Actomyosin Contractility Regulate the Segmentation Clock and Spatio-Temporal Somite Cleft Formation during Chick Embryo Somitogenesis. Cells, 11(13):2003. DOI: 3390/cells11132003
Quaresma MC, Botelho HM, Pankonien I, Rodrigues CS, Pinto MC, Costa PR, Duarte A, Amaral MD (2022) Exploring YAP1-centered networks linking dysfunctional CFTR to epithelial–mesenchymal transition. Life Sci Alliance, 5(9):e202101326. DOI: 26508/lsa.202101326
Mira AF, Marques L, Magalhães S, Rodrigues LR (2022) A Method to Measure the Damage Caused by Cell-Sucking Herbivores. In: Duque, P., Szakonyi, D. (eds) Environmental Responses in Plants. Methods in Molecular Biology, 2494:299-312. DOI: 1007/978-1-0716-2297-1_21
Tomás A, da Fonseca IP, Valkenburg T, Rebelo MT (2022) Parasite island syndromes in the context of nidicolous ectoparasites: Fleas (Insecta: Siphonaptera) in wild passerine birds from Azores Archipelago. Parasitol Int, 89:102564. DOI: 1016/j.parint.2022.102564
Lim SH, Snider J, Birimberg-Schwartz L, Ip W, Serralha JC, Botelho HM, Lopes-Pacheco M, Pinto MC, Moutaoufik MT, Zilocchi M, Laselva O, Esmaeili M, Kotlyar M, Lyakisheva A, Tang P, Vázquez LL, Akula I, Aboualizadeh F, Wong V, Grozavu I, Opacak-Bernardi T, Yao Z, Mendoza M, Babu M, Jurisica I, Gonska T, Bear CE, Amaral MD, Stagljar I (2022) CFTR interactome mapping using the mammalian membrane two-hybrid high-throughput screening system. Mol Syst Biol, 18(2):e10629. DOI: 15252/msb.202110629
Murtinheira F, Migueis M, Letra-Vilela R, Diallo M, Quezada A, Valente CA, Oliva A, Rodriguez C, Martin V, Herrera F (2022) Sacsin Deletion Induces Aggregation of Glial Intermediate Filaments. Cells, 11(2):299. DOI: 3390/cells11020299
Rodrigues AV, Valério-Bolas A, Alexandre-Pires G, Pereira MA, Nunes T, Ligeiro D, da Fonseca IP, Santos-Gomes G (2022) Zoonotic Visceral Leishmaniasis: New Insights on Innate Immune Response by Blood Macrophages and Liver Kupffer Cells to Leishmania infantum Parasites. Biology, 11(1):100. DOI: 3390/biology11010100
da Silva ACN, Costa AL, Teixeira A, Alpoim-Moreira J, Fernandes C, Fradinho MJ, Rebordão MR, Silva E, da Silva JF, Bliebernicht M, Alexandre-Pires G, Ferreira-Dias G (2022) Collagen and Microvascularization in Placentas From Young and Older Mares. Front Vet Sci, 8:772658. DOI: 3389/fvets.2021.772658
Pinto MC, Botelho HM, Silva IAL, Railean V, Neumann B, Pepperkok R, Schreiber R, Kunzelmann K, Amaral MD (2022) Systems Approaches to Unravel Molecular Function: High-content siRNA Screen Identifies TMEM16A Traffic Regulators as Potential Drug Targets for Cystic Fibrosis. J Mol Biol, 434(5):167436. DOI: 1016/j.jmb.2021.167436
Lopes-Pacheco M, Bacalhau M, Ramalho SS, Silva IAL, Ferreira FC, Carlile GW, Thomas DY, Farinha CM, Hanrahan JW, Amaral MD (2022) Rescue of Mutant CFTR Trafficking Defect by the Investigational Compound MCG1516A. Cells, 11(1):136. DOI: 3390/cells11010136
Ramalho SS, Silva IAL, Amaral MD, Farinha CM (2022) Rare Trafficking CFTR Mutations Involve Distinct Cellular Retention Machineries and Require Different Rescuing Strategies. Int J Mol Sci, 23(1):24. DOI: 3390/ijms23010024
Simões FB, Kmit A, Amaral MD (2021) Cross-talk of inflammatory mediators and airway epithelium reveals the cystic fibrosis transmembrane conductance regulator as a major target. ERJ Open Res, 7(4):00247-2021. DOI: 1183/23120541.00247-2021
Pinto MC, Quaresma MC, Silva IAL, Railean V, Ramalho SS, Amaral MD (2021) Synergy in Cystic Fibrosis Therapies: Targeting SLC26A9. Int J Mol Sci, 22(33):13064. DOI: 3390/ijms222313064
Moreira GG, Cantrelle F-X, Quezada A, Carvalho FS, Cristóvão JS, Sengupta U, Puangmalai N, Carapeto AP, Rodrigues MS, Cardoso I, Fritz G, Herrera F, Kayed R, Landrieu I, Gomes CM (2021) Dynamic interactions and Ca²⁺-binding modulate the holdase-type chaperone activity of S100B preventing tau aggregation and seeding. Nat Commun, 12(1):6292. DOI: 1038/s41467-021-26584-2
Casimiro MH, Pereira A, Leal JP, Rodrigues G, Ferreira LM (2021) Chitosan/PVA Based Membranes Processed by Gamma Radiation as Scaffolding Materials for Skin Regeneration. Membranes, 11(8):561. DOI: 3390/membranes11080561
Godinho-Pereira J, Garcia AR, Figueira I, Malhó R, Brito MA (2021) Behind Brain Metastases Formation: Cellular and Molecular Alterations and Blood-Brain Barrier Disruption. Int J Mol Sci, 22(13):7057. DOI: 3390/ijms22137057
Santos L, Mention K, Cavusoglu-Doran K, Sanz DJ, Bacalhau M, Lopes-Pacheco M, Harrison PT, Farinha CM (2021) Comparison of Cas9 and Cas12a CRISPR editing methods to correct the W1282X-CFTR mutation. J Cyst Fibros, in press. DOI: 1016/j.jcf.2021.05.014
Seabra SG, Rodrigues ASB, Silva SE, Neto AC, Pina-Martins F, Marabuto E, Thompson V, Wilson MR, Yurtsever S, Halkka A, Rebelo MT, Borges PAV, Quartau JA, Jiggins CD, Paulo OS (2021) Population structure, adaptation and divergence of the meadow spittlebug, Philaenus spumarius (Hemiptera, Aphrophoridae), revealed by genomic and morphological data. PeerJ, 9:e11425. DOI: 7717/peerj.11425
Silva IAL, Railean V, Duarte A, Amaral MD (2021) Personalized Medicine Based on Nasal Epithelial Cells: Comparative Studies with Rectal Biopsies and Intestinal Organoids. J Pers Med, 11(5):421. DOI: 3390/jpm11050421
Figueira I, Godinho-Pereira J, Galego S, Maia J, Haskó J, Molnár K, Malhó R, Costa-Silva B, Wilhelm I, Krizbai IA, Brito MA (2021) MicroRNAs and Extracellular Vesicles as Distinctive Biomarkers of Precocious and Advanced Stages of Breast Cancer Brain Metastases Development. Int J Mol Sci. 22(10):5214. DOI: 3390/ijms22105214
Ferreira M, Pinto SN, Aires-da-Silva F, Bettencourt A, Aguiar SI, Gaspar MM (2021) Liposomes as a Nanoplatform to Improve the Delivery of Antibiotics into Staphylococcus aureus Pharmaceutics, 13(3):321. DOI: 10.3390/pharmaceutics13030321
Figueira I, Galego S, Custódio-Santos T, Vicente R, Molnár K, Haskó J, Malhó R, Videira M, Wilhelm I, Krizbai I, Brito MA (2021) Picturing Breast Cancer Brain Metastasis Development to Unravel Molecular Players and Cellular Crosstalk. Cancers, 13(4):910. DOI: 3390/cancers13040910
Serrazina S, Machado H, Costa RL, Duque P, Malhó R (2021) Expression of Castanea crenata allene oxide synthase in Arabidopsis improves the defense to Phytophthora cinnamomi. Front Plant Sci, 12:628697. DOI: 3389/fpls.2021.628697
Galego S, Kauppila LA, Malhó R, Pimentel J, Brito MA (2021) Myocyte Enhancer Factor 2C as a New Player in Human Breast Cancer Brain Metastases. Cells, 10(2):378. DOI: 3390/cells10020378
Hagemeijer MC, Vonk AM, Awatade NT, Silva IAL, Tischer C, Hilsenstein V, Beekman JM, Amaral MD, Botelho HM (2020) An open-source high-content analysis workflow for CFTR function measurements using the forskolin-induced swelling assay. Bioinformatics, btaa1073. DOI: 1093/bioinformatics/btaa1073
Silva IAL, Duarte A, Marson FAL, Centeio R, Doušová T, Kunzelmann K, Amaral MD (2020) Assessment of distinct electrophysiological parameters in rectal biopsies for the choice of the best diagnosis/prognosis biomarkers for cystic fibrosis. Front Physiol, 11:604580. DOI: 3389/fphys.2020.604580
Tomás A, da Fonseca IP, Valkenburg T, Rebelo MT (2021) Louse flies in Azorean and mainland populations of four Passeriformes species: A new perspective to parasite Island syndromes. Int J Parasitol Parasites Wildl, 14:33-40. DOI: 1016/j.ijppaw.2020.12.004
Quaresma MC, Pankonien I, Clarke LA, Sousa LS, Silva IAL, Railean V, Doušová T, Fuxe J, Amaral MD (2020) Mutant CFTR drives TWIST1 mediated epithelial-mesenchymal transition. Cell Death Dis, 11(10):920. DOI: 1038/s41419-020-03119-z
Sousa L, Pankonien I, Simões FB, Chanson M, Amaral MD (2020) Impact of KLF4 on Cell Proliferation and Epithelial Differentiation in the Context of Cystic Fibrosis. Int J Mol Sci, 21(18):6717. DOI: 3390/ijms21186717
Caperta AD, Róis AS, Teixeira G, Garcia‐Caparros P, Flowers TJ (2020) Secretory structures in plants: Lessons from the Plumbaginaceae on their origin, evolution and roles in stress tolerance. Plant Cell Environ, 43(12), 2912–2931. DOI: 1111/pce.13825
Silva IAL, Doušová T, Ramalho S, Centeio R, Clarke LA, Railean V, Botelho HM, Holubová A, Valášková I, Yeh J-T, Hwang T-C, Farinha CM, Kunzelmann K, Amaral MD (2020) Organoids as a personalized medicine tool for ultra-rare mutations in cystic fibrosis: The case of S955P and 1717-2A>G. Biochim Biophys Acta Mol Basis Dis, 1866(11):165905. DOI: 1016/j.bbadis.2020.165905
Santos JD, Pinto FR, Ferreira JF, Amaral MD, Zaccolo M, Farinha CM (2020) Cytoskeleton regulators CAPZA2 and INF2 associate with CFTR to control its plasma membrane levels under EPAC1 activation. Biochem J, 477(13):2561–2580. DOI: 1042/BCJ20200287
Lopes-Pacheco M, Silva IAL, Turner MJ, Carlile GW, Sondo E, Thomas DY, Pedemonte N, Hanrahan JW, Amaral MD (2020) Characterization of the mechanism of action of RDR01752, a novel corrector of F508del-CFTR. Biochem Pharmacol, 180, 114133. DOI: 1016/j.bcp.2020.114133
Sousa L, Pankonien I, Clarke LA, Silva I, Kunzelmann K, Amaral MD (2020) KLF4 acts as a wt-CFTR suppressor through an AKT-mediated pathway. Cells, 9(7). DOI: 3390/cells9071607
Vonk AM, van Mourik P, Ramalho AS, Silva IAL, Statia M, Kruisselbrink E, Suen SWF, Dekkers JF, Vleggaar FP, Houwen RHJ, Mullenders J, Boj SF, Vries R, Amaral MD, de Boeck K, van der Ent CK, Beekman JM (2020). Protocol for application, standardization and validation of the forskolin-induced swelling assay in cystic fibrosis human colon organoids. STAR Protoc, 1(1):100019. DOI: 1016/j.xpro.2020.100019
Khmelinskaia A, Marquês JMT, Bastos AEP, Antunes CAC, Bento-Oliveira A, Scolari S, Lobo GMS, Malhó R, Herrmann A, Marinho HS, de Almeida RFM (2020) Liquid-ordered phase formation by mammalian and yeast sterols: a common feature with organizational differences. Front Cell Dev Biol, 8, 337. DOI: 3389/fcell.2020.00337
Figueiredo AM, Villacampa P, Diéguez-Hurtado R, Lozano JJ, Kobialka P, Cortazar AR, Martinez-Romero A, Angulo-Urarte A, Franco CA, Claret M, Aransay AM, Adams RH, Carracedo A, Graupera M (2020) Phosphoinositide 3-Kinase–Regulated Pericyte Maturation Governs Vascular Remodeling. Circulation, 142:688–704. DOI: 1161/CIRCULATIONAHA.119.042354
Pinto MC, Schreiber R, Lérias J, Ousingsawat J, Duarte A, Amaral M, Kunzelmann K (2020) Regulation of TMEM16A by CK2 and its role in cellular proliferation. Cells, 9(5). DOI: 3390/cells9051138
Cortinhas A, Caperta AD, Teixeira G, Carvalho L, Abreu MM (2020) Harnessing sediments of coastal aquaculture ponds through technosols construction for halophyte cultivation using saline water irrigation. J Environ Manage, 261, 109907. DOI: 1016/j.jenvman.2019.109907
Correia S, Santos M, Glińska S, Gapińska M, Matos M, Carnide V, Schouten R, Silva AP, Gonçalves B (2020) Effects of exogenous compound sprays on cherry cracking: skin properties and gene expression. J Sci Food Agric, 100(7), 2911–2921. DOI: 1002/jsfa.10318
Marques NT, Filipe A, Pinto P, Barroso J, Trindade H, Power DM, Figueiredo AC (2020) Trichome density in relation to volatiles emission and 1,8-cineole synthase gene expression in Thymus albicans vegetative and reproductive organs. Chemistry & Biodiversity, 17(3), e1900669. DOI: 1002/cbdv.201900669
Sereno M, Haskó J, Molnár K, Medina SJ, Reisz Z, Malhó R, Videira M, Tiszlavicz L, Booth SA, Wilhelm I, Krizbai IA, Brito MA (2020) Downregulation of circulating miR 802-5p and miR 194-5p and upregulation of brain MEF2C along breast cancer brain metastasization. Mol Oncol, 14(3):520–538. DOI: 1002/1878-0261.12632
Amaral MD, Beekman JM (2020) Activating alternative chloride channels to treat CF: Friends or Foes?: Report on the Meeting of the Basic Science Working Group in Dubrovnik, Croatia. J Cyst Fibros, 19(1):11–15. DOI: 1016/j.jcf.2019.10.005
Amaral MD, Hutt DM, Tomati V, Botelho HM, Pedemonte N (2020) CFTR processing, trafficking and interactions. J Cyst Fibros, 19 Suppl 1, S33–S36. DOI: 1016/j.jcf.2019.10.017
Simão M, Moço M, Marques L, Santos R (2020) Characterization of the glycans involved in sea urchin Paracentrotus lividus reversible adhesion. Marine Biology, 167(9), 125. DOI: 1007/s00227-020-03707-9
Garcia AR, Godinho-Pereira J, Figueira I, Malhó R, Brito MA (2019) Replicating the blood- brain barrier properties in an in vitro model: effects of hydrocortisone and/or shear stress. Archives of Anatomy, 8 (2):1-20.
Vaz Dias F, Serrazina S, Vitorino M, Marchese D, Heilmann I, Godinho M, Rodrigues M, Malhó R (2019) A role for diacylglycerol kinase 4 in signalling crosstalk during Arabidopsis pollen tube growth. New Phytol 222(3), 1434–1446. DOI: 1111/nph.15674
Palma E, Botelho HM, Morais GR, Rodrigues I, Santos IC, Campello MPC, Raposinho P, Belchior A, Gomes SS, Araújo MF, Correia I, Ribeiro N, Gama S, Mendes F, Paulo A (2019) Unravelling the antitumoral potential of novel bis(thiosemicarbazonato) Zn(II) complexes: structural and cellular studies. J Biol Inorg Chem, 24(1):71–89. DOI: 1007/s00775-018-1629-6
Awatade NT, Ramalho S, Silva IAL, Felício V, Botelho HM, de Poel E, Vonk A, Beekman JM, Farinha CM, Amaral MD (2019) R560S: A class II CFTR mutation that is not rescued by current modulators. J Cyst Fibros, 18(2):182–189. DOI: 1016/j.jcf.2018.07.001
Santos JD, Canato S, Carvalho AS, Botelho HM, Aloria K, Amaral MD, Matthiesen R, Falcão AO, Farinha CM (2019) Folding status is determinant over traffic-competence in defining CFTR interactors in the endoplasmic reticulum. Cells, 8(4):353. DOI: 3390/cells8040353
Loureiro CA, Santos JD, Matos AM, Jordan P, Matos P, Farinha CM, Pinto FR (2019) Network biology identifies novel regulators of CFTR trafficking and membrane stability. Front Pharmacol, 10, 619. DOI: 3389/fphar.2019.00619
Patel W, Moore PJ, Sassano MF, Lopes-Pacheco M, Aleksandrov AA, Amaral MD, Tarran R, Gray MA (2019) Increases in cytosolic Ca²⁺ induce dynamin- and calcineurin-dependent internalisation of CFTR. Cell Mol Life Sci, 76(5):977–994. DOI: 1007/s00018-018-2989-3
Simões FB, Quaresma MC, Clarke LA, Silva IAL, Pankonien I, Railean V, Kmit A, Amaral MD (2019) TMEM16A chloride channel does not drive mucus production. Life Sci Alliance, 2(6). DOI: 26508/lsa.201900462
Dias JNR, Aguiar SI, Pereira DM, André AS, Gano L, Correia JDG, Carrapiço B, Rütgen B, Malhó R, Peleteiro C, Goncalves J, Rodrigues CMP, Gil S, Tavares L, Aires-da-Silva F (2018) The histone deacetylase inhibitor panobinostat is a potent antitumor agent in canine diffuse large B-cell lymphoma. Oncotarget, 9(47):28586-28598. DOI: 18632/oncotarget.25580
Olivença DV, Uliyakina I, Fonseca LL, Amaral MD, Voit EO, Pinto FR (2018) A mathematical model of the phosphoinositide pathway. Sci Rep, 8(1):3904. DOI: 1038/s41598-018-22226-8
Sampaio-Pinto V, Rodrigues SC, Laundos TL, Silva ED, Vasques-Nóvoa F, Silva AC, Cerqueira RJ, Resende TP, Pianca N, Leite-Moreira A, D’Uva G, Thorsteinsdóttir S, Pinto-do-Ó P, Nascimento DS (2018) Neonatal apex resection triggers cardiomyocyte proliferation, neovascularization and functional recovery despite local fibrosis. Stem Cell Reports, 10(3):860–874. DOI: 1016/j.stemcr.2018.01.042
Casimiro MH, Gomes SR, Rodrigues G, Leal JP, Ferreira LM (2018) Chitosan/poly(vinylpyrrolidone) matrices obtained by gamma-irradiation for skin scaffolds: characterization and preliminary cell response studies. Materials (Basel), 11(12). DOI: 3390/ma11122535
Lérias JR, Pinto MC, Botelho HM, Awatade NT, Quaresma MC, Silva IAL, Wanitchakool P, Schreiber R, Pepperkok R, Kunzelmann K, Amaral MD (2018) A novel microscopy-based assay identifies extended synaptotagmin-1 (ESYT1) as a positive regulator of anoctamin 1 traffic. Biochim Biophys Acta Mol Cell Res, 1865(2):421–431. DOI: 1016/j.bbamcr.2017.11.009
Sebastiana M, da Silva AB, Matos AR, Alcântara A, Silvestre S, Malhó R (2018) Ectomycorrhizal inoculation with Pisolithus tinctorius reduces stress induced by drought in cork oak. Mycorrhiza, 28(3):247–258. DOI: 1007/s00572-018-0823-2
Camelo C, Vilas-Boas F, Cepeda AP, Real C, Barros-Martins J, Pinto F, Soares H, Marinho HS, Cyrne, L (2017) Opi1p translocation to the nucleus is regulated by hydrogen peroxide in Saccharomyces cerevisiae. Yeast, 34(9):383–395. DOI: 1002/yea.3240
Gomes S, Rodrigues G, Martins G, Henriques C, Silva JC (2017) Evaluation of nanofibrous scaffolds obtained from blends of chitosan, gelatin and polycaprolactone for skin tissue engineering. Int J Biol Macromol, 102, 1174-1185. DOI: 1016/j.ijbiomac.2017.05.004
Nunes AM, Wuebbles RD, Sarathy A, Fontelonga TM, Deries M, Burkin DJ, Thorsteinsdóttir S (2017) Impaired fetal muscle development and JAK-STAT activation mark disease onset and progression in a mouse model for merosin-deficient congenital muscular dystrophy. Hum Mol Genet, 26(11):2018–2033. DOI: 1093/hmg/ddx083
Igreja S, Clarke LA, Botelho HM, Marques L, Amaral MD (2016) Correction of a cystic fibrosis splicing mutation by antisense oligonucleotides. Human Mutat, 37(2):209–215. DOI: 1002/humu.22931
Gonçalves AB, Thorsteinsdóttir S, Deries M (2016) Rapid and simple method for in vivo ex utero development of mouse embryo explants. Differentiation, 91(4–5):57–67. DOI: 1016/j.diff.2015.12.002
Amaral MD, Farinha CM, Matos P, Botelho HM (2016) Investigating alternative transport of integral plasma membrane proteins from the ER to the Golgi: lessons from the cystic fibrosis transmembrane conductance regulator (CFTR). Methods Mol Biol, 1459, 105–126. DOI: 1007/978-1-4939-3804-9_7
Lebesgue N, da Costa G, Ribeiro RM, Ribeiro-Silva C, Martins GG, Matranga V, Scholten A, Cordeiro C, Heck AJR, Santos R (2016) Deciphering the molecular mechanisms underlying sea urchin reversible adhesion: A quantitative proteomics approach. J Proteomics, 138, 61-71. DOI: 1016/j.jprot.2016.02.026
Silva AC, Rodrigues SC, Caldeira J, Nunes AM, Sampaio-Pinto V, Resende TP, Oliveira MJ, Barbosa MA, Thorsteindóttir S, Pinto-do-Ó P (2016) Three-dimensional scaffolds of fetal decellularized hearts exhibit enhanced potential to support cardiac cells in comparison to the adult. Biomaterials, 104, 52–64. DOI: 1016/j.biomaterials.2016.06.062
Luzio A, Matos M, Santos D, Fontaínhas-Fernandes AA, Monteiro SM, Coimbra AM (2016) Disruption of apoptosis pathways involved in zebrafish gonad differentiation by 17α-ethinylestradiol and fadrozole exposures. Aquat Toxicol, 177, 269–284. DOI: 1016/j.aquatox.2016.05.029
de Almeida PG, Pinheiro GG, Nunes AM, Gonçalves AB, Thorsteinsdóttir S (2016) Fibronectin assembly during early embryo development: A versatile communication system between cells and tissues. Dev Dyn, 245(4):520-535. DOI: 1002/dvdy.24391
Ramalho AS, Clarke LA, Sousa M, Felício V, Barreto C, Lopes C, Amaral MD (2016) Comparative ex vivo, in vitro and in silico analyses of a CFTR splicing mutation: Importance of functional studies to establish disease liability of mutations. J Cyst Fibros, 15(1):21–33. DOI: 1016/j.jcf.2015.02.002
Saavedra L, Catarino R, Heinz T, Heilmann I, Bezanilla M, Malhó RM (2015) Phosphatase and tensin homolog (PTEN) is a growth repressor of both rhizoid and gametophore development in the moss Physcomitrella patens. Plant Physiol, 169(4):2572-2586. DOI: 1104/pp.15.01197
Botelho HM, Uliyakina I, Awatade NT, Proença MC, Tischer C, Sirianant L, Kunzelmann K, Pepperkok R, Amaral MD (2015) Protein traffic disorders: an effective high-throughput fluorescence microscopy pipeline for drug discovery. Sci Rep, 5(1):9038. DOI: 1038/srep09038
Clarke LA, Botelho HM, Sousa L, Falcão AO, Amaral MD (2015) Transcriptome meta-analysis reveals common differential and global gene expression profiles in cystic fibrosis and other respiratory disorders and identifies CFTR regulators. Genomics, 106(5):268–277. DOI: 1016/j.ygeno.2015.07.005
Loureiro CA, Matos AM, Dias-Alves Â, Pereira JF, Uliyakina I, Barros P, Amaral MD, Matos P (2015) A molecular switch in the scaffold NHERF1 enables misfolded CFTR to evade the peripheral quality control checkpoint. Sci Signal, 8(377):ra48. DOI: 1126/scisignal.aaa1580
Verkman AS, Edelman A, Amaral M, Mall MA, Beekman JM, Meiners T, Galietta LJV, Bear CE (2015) Finding new drugs to enhance anion secretion in cystic fibrosis: Toward suitable systems for better drug screening. Report on the pre-conference meeting to the 12th ECFS Basic Science Conference, Albufeira, 25-28 March 2015. J Cyst Fibros, 14(6):700–705. DOI: 1016/j.jcf.2015.10.001
Amaral MD, Balch WE (2015) Hallmarks of therapeutic management of the cystic fibrosis functional landscape. J Cyst Fibros, 14(6):687–699. DOI: 1016/j.jcf.2015.09.006

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