IGC Imaging Platforms
Scientific support units at the Instituto Gulbenkian De Ciência provide technology driven expertise and services. Two units are available to support driven research with state-of-the-art technologies and up to date trained personnel.
Implementing new applications and protocols. Accelerating research.
Advanced Imaging Facility
Electron Microscopy Facility
Advanced Imaging Facility – IGC
The IGC’s Advanced Imaging Unit offers state-of-the-art light microscopy and bioimaging services, housing 16 high-end systems with confocal/2photon, high-throughput, super-res & mesoscopy (light-sheet & OPT).
Services include sample prep., and training with instrumentation & bioimage analysis (high-end workstations available with commercial & free software). The AIU is open-access and serves the IGC, academia and companies.
Team
Gabriel G. Martins
Head
Alexandre Lopes
Staff
Manuel Tanqueiro
Staff
Mariana Ferreira
part-time Staff
Patrícia Rodrigues
Staff
Resources
- Confocal Systems
- Zeiss LSM980/Airyscan2
- Leica Stellaris w/Lightning
- 3i Marianas Spinning Disk
- Leica High-speed Spinning Disk
- 3i Marianas-2BSL Spinning Disk + TIRF
- Andor Dragonfly Spinning Disk
- Andr W1 Spinning Disk
- Widefield Systems
- Zeiss Imager2/Apotome2
- Nikon HTM-HCS
- ZeissLUMAR + Aequoria
- Super Resolution Microscopes
- Deltavision OMX SIM-SR
- ONI Nanoimager
- Light-sheet Fluorescence Microscopes
- Zeiss Z.1 Lightsheet
- Mesoscope Prototype
- Image analysis software
- Imaris
- ARIVIS
- AMIRA
- Huygens
- Matlab
Offered Technologies
- Deconvolution Widefield Microscopy (DWM)
- Laser Scanning Confocal Microscopy (LSCM/CLSM)
- Spinning disk confocal microscopy (SDCM)
- Structured illumination microscopy (SIM)
- Two-photon microscopy (2P)
- Total internal reflection fluorescence microscopy (TIRF)
- Single Molecule localization microscopy (SMLM)
- Light-Sheet Mesoscopic Imaging (SPIM or dSLSM)
- Optical projection tomography (OPT)
- Second/Third Harmonics Generation (SHG/THG)
- High Throughput Microscopy/High Content Sreening (HTM/HCS)
- Fluorescence Resonance Energy Transfer (FRET)
- Fluorescence Recovery After Photobleaching (FRAP)
- Intravital Microscopy (IVM)
- Microdissection
- Imaging at Biosafety Level >1
- Photomanipulation
- Expansion Microscopy
- Tissue Clearing (TC)
- High Performance Workstation
- High Performance Workstation with High-end GPU
Publications
- Bota C, Martins GG, Lopes SS. Dand5 is involved in zebrafish tailbud cell movement. Front Cell Dev Biol. 2023 Jan 9;10:989615. doi: 10.3389/fcell.2022.989615. PMID: 36699016; PMCID: PMC9869157.
- Schmied C, Nelson MS, Avilov S, Bakker GJ, Bertocchi C, Bischof J, Boehm U, Brocher J, Carvalho M, Chiritescu C, Christopher J, Cimini BA, Conde-Sousa E, Ebner M, Ecker R, Eliceiri K, Fernandez-Rodriguez J, Gaudreault N, Gelman L, Grunwald D, Gu T, Halidi N, Hammer M, Hartley M, Held M, Jug F, Kapoor V, Koksoy AA, Lacoste J, Dévédec SL, Guyader SL, Liu P, Martins GG, Mathur A, Miura K, Montero Llopis P, Nitschke R, North A, Parslow AC, Payne-Dwyer A, Plantard L, Ali R, Schroth-Diez B, Schütz L, Scott RT, Seitz A, Selchow O, Sharma VP, Spitaler M, Srinivasan S, Strambio-De-Castillia C, Taatjes D, Tischer C, Jambor HK. Community-developed checklists for publishing images and image analyses. ArXiv [Preprint]. 2023 Sep 14:arXiv:2302.07005v2. Update in: Nat Methods. 2023 Sep 14;: PMID: 36824427; PMCID: PMC9949169.
- Gaudreault Nathalie, Ali Rizwan, Avilov Sergiy V, Bagley Steve, Bammann Rodrigo R., Barachati Fabio, Martins Gabriel G et al. Illumination Power, Stability, and Linearity Measurements for Confocal and Widefield Microscopes V.2. protocols.io. 2023 mar.
- Anastasiia Lozovska, Artemis G. Korovesi, André Dias, Alexandre Lopes, Donald A. Fowler, Gabriel G. Martins, Ana Nóvoa, Moisés Mallo. Tgfbr1 controls developmental plasticity between the hindlimb and external genitalia by remodeling their regulatory landscape. BioArXiv [Preprint]. 2022 Nov.
- Anastasiia Lozovska, Ana Nóvoa, Ying-Yi Kuo, Arnon D. Jurberg, Gabriel G. Martins, Anna-Katerina Hadjantonakis, Moises Mallo. Tgfbr1 regulates lateral plate mesoderm and endoderm reorganization during the trunk to tail transition. BioArXiv [Preprint]. 2022 Nov.
- Vale-Costa S, Etibor TA, Brás D, Sousa AL, Ferreira M, Martins GG, Mello VH, Amorim MJ. ATG9A regulates the dissociation of recycling endosomes from microtubules to form liquid influenza A virus inclusions. PLoS Biol. 2023 Nov 20;21(11):e3002290. doi: 10.1371/journal.pbio.3002290. PMID: 37983294; PMCID: PMC10695400.
- Hidalgo-Cenalmor I, Pylvänäinen JW, G Ferreira M, Russell CT, Saguy A, Arganda-Carreras I, Shechtman Y; AI4Life Horizon Europe Program Consortium; Jacquemet G, Henriques R, Gómez-de-Mariscal E. DL4MicEverywhere: deep learning for microscopy made flexible, shareable and reproducible. Nat Methods. 2024 May 17. doi: 10.1038/s41592-024-02295-6. Epub ahead of print. PMID: 38760611.
Education
Our staff organizes and participates in different courses and hands-on workshops related to the technologies available at our Platform.
FUTURE EVENTS:
- QuPath PPBI course (broadcast from IGC). May 27-29th.
- BAM – Basics on Advanced Microscopy course
Job Shadowing
- 3D imaging
- Mesoscopy
- Tissue Clearing
- Expansion Microscopy
- 3D image analyis
- OPT
- Correlated Imaging
- Training in Quality Control (QC)
- Guidance in Management
Electron Microscopy – IGC
The EM Unit is specialized in transmission electron microscopy for biological samples but we are open to all scientific disciplines. Our aim is to provide high-quality EM services, training, and access to specialized equipment for all IGC, external academic or corporate users.
We tailor, optimize, and develop methods adapted to your scientific questions. Please consult the equipment and techniques listed under the tabs “Equipment” and “Services & Techniques”. If you have questions and/or want to start a project please contact the unit.
Team
Erin Tranfield
Head
Ana Sousa
Staff
Beatriz Tomaz
Staff
Resources
- Transmission Electron Microscopes
- FEI Tecnai G2 Spirit BioTWIN
- Hitachi H-7650
- Widefield Systems
- Olympus IX81 inverted fluorescence microscope
- Diverse Stereomicroscopes
- Other REsources
- Leica UC7 / FC7 Ultramicrotome
- Wholwend Compact 2 High Pressure Freezer
- Leica AFS2 with the Leica EM FSP Robot
- PELCO Biowave Pro+ Sample Processing System With SteadyTemp Pro
- Q150T ES High Vacuum Carbon/Metal Coater
- PELCO easiGlow Glow Discharge Cleaning System
- Image analysis software
Offered Technologies
- High Performance Workstation
- High Performance Workstation with High-end GPU
Publications
- Sónia Gomes Pereira, Ana Laura Sousa, Catarina Nabais, Tiago Paixão, Alexander J. Holmes, Martin Schorb, Gohta Goshima, Erin M. Tranfield, Jörg D. Becker, Mónica Bettencourt-Dias (2021) The 3D architecture and molecular foundations of de novo centriole assembly via bicentrioles. Science Direct 31 (19):4340-4353.e7
- Ana Laura Sousa, Joana Rodrigues Lóios, Pedro Faísca, Erin M. Tranfield (2021) Chapter 2 – The Histo-CLEM Workflow for tissues of model organisms. Science Direct 162:13-37
- Carvalho, A.S.; Moraes, M.C.S.; Hyun Na, C.; Fierro-Monti, I.; Henriques, A.; Zahedi, S.; Bodo, C.; Tranfield, E.M.; Sousa, A.L.; Farinho, A.; Rodrigues, L.V.; Pinto, P.; Bárbara, C.; Mota, L.; Abreu, T.T.d.; Semedo, J.; Seixas, S.; Kumar, P.; Costa-Silva, B.; Pandey, A.; Matthiesen, R. (2020) Is the Proteome of Bronchoalveolar Lavage Extracellular Vesicles a Marker of Advanced Lung Cancer?. cancers 12 (11)
- Costa, Júlia; Pronto-Laborinho, Ana; Pinto, Susana Gromicho, Marta; Bonucci, Sara; Tranfield, Erin; Correia, Catarina; Alexandre, Bruno; M. de Carvalho, Mamede (2020) Investigating LGALS3BP/90 K glycoprotein in the cerebrospinal fluid of patients with neurological diseases. Scientific Reports 10 (1)
- Reichmann, Nathalie T. Tavares, Andreia C. Saraiva, Bruno M. Jousselin, Ambre Reed, Patricia Pereira, Ana R. Monteiro, João M. Sobral, Rita G. VanNieuwenhze, Michael S. Fernandes, Fábio Pinho, Mariana G. (2019) SEDS–bPBP pairs direct lateral and septal peptidoglycan synthesis in Staphylococcus aureus. Nature Microbiology 4 (8)
- Alenquer, Marta Vale-Costa, Sílvia Etibor, Temitope Akhigbe Ferreira, Filipe Sousa, Ana Laura Amorim, Maria João (2019) Influenza A virus ribonucleoproteins form liquid organelles at endoplasmic reticulum exit sites. Nature Communications 10 (1)
- Fernandes CG, Martins D, Hernandez G, Sousa AL, Freitas C, Tranfield EM (2019) Temporal and spatial regulation of protein cross-linking by the pre-assembled substrates of a Bacillus subtilis spore coat transglutaminase. PLoS Genet 15 (4):e1007912
- Fleck, R.A., Humbel, B.M., Humbel, B.M., Schwarz, H., Tranfield, E.M. and Fleck, R.A. (2019) Chemical Fixation. Wiley Online Library 191-221
- Erin M Tranfield, Leandro Lemgruber Transmission electron microscopy. IOP Publishing 1 (24):I.5.a-1 to I.5.a-16
Education
Our staff organizes and participates in different courses and hands-on workshops related to the technologies available at our Platform.
FUTURE EVENTS: