Total Internal Reflection Fluorescence (TIRF) microscopy is a powerful tool for studying dynamic biological processes that occur at or near the plasma membrane or during in vitro reconstitutions. An evanescent field excites fluorophores in a thin slice of 100 to 200 nm above the glass surface, achieving a sectioning 5 to 10 times sharper than confocal microscopy. By minimizing background and achieving a high signal-to-noise ratio, dynamic processes can be monitored precisely and with low cytotoxicity. In the past, TIRF was generally conducted one color at a time, limiting the extent of interpretation when following complex mechanisms involving multiple molecular partners.

In this workshop, we demonstrate Abbelight simultaneous multicolor TIRF imaging, enabled by the SAFe platform workflow, designed to combine versatility, automation, and precision. Our system enables up to 4-colors imaging, both simultaneous and sequential, giving researchers the flexibility to study complex phenomenon involving multiple molecular partners or structures within the same field of view.

Based on automatic calibration, Abbelight NEO LiveImagingTM software predicts the penetration depth based on the illumination angle chosen by the user. This gives users precise control over the excitation depth and ensures reproducible imaging and quantifications across experimental sessions. Overall, Abbelight SAFe simultaneous multicolor TIRF imaging enables the investigation of protein colocalization, receptor clustering, membrane trafficking, phase separation, biocondensates dynamics, signal transduction, and many other dynamic membrane processes.

Modern, high throughput 3D microscopy not only requires solutions for rapid image acquisition, but also for scalable, on-the-fly image data transfer, image processing and content analysis. This workshop presents an integrated imaging and computing workflow combining the Bruker Luxendo light-sheet microscope with the Acquifer HIVE data management, a multi-user, high performance computing platform.

Multi-camera, multi-view light sheet microscopes enable fast volumetric imaging of complex 3D biological samples such as spheroids, organoids, and developing organisms, delivering high optical efficiency and gentle illumination across multiple imaging dimensions. To match this acquisition speed, imaging data is directly streamed to Acquifer HIVE, where it is centrally stored, processed, and made immediately available for analysis and word-wide collaboration, all in an integrated workflow made available by smart Hardware and Software implementation. The workshop demonstrates how end to end optimization—from image acquisition to compute accelerated processing—removes data bottlenecks and enables efficient, reproducible 3D microscopy workflows for microscopists, imaging facilities and translational research.

Biological processes occur across spatial and temporal scales that increasingly challenge conventional three dimensional imaging approaches. Capturing fast physiological dynamics in living systems requires volumetric imaging methods that provide high temporal resolution without compromising spatial context. The recently introduced Lightfield 4D technology, integrated into the ZEISS LSM 910 and LSM 990 platforms, addresses this challenge by enabling instant volumetric acquisition within a single camera exposure.

Implemented as a modular component of the LSM 910 and LSM 990, ZEISS Lightfield 4D allows for mixed modality workflows where images of the sample specimen can be captured at high temporal resolution with the Lightfield and then LSM for higher spatial resolution, spectral multiplex imaging, molecular dynamics measurements, and photomanipulation. This flexibility supports experiments across a wide range of sample types, including cultured cells, organoids, tissue sections, and small model organisms.

In this presentation, we will introduce the principles underlying Lightfield 4D imaging on the ZEISS LSM 910 and LSM 990 platforms and discuss how instant volumetric acquisition reshapes experimental design for dynamic biological systems. Application examples will be shown to illustrate how high speed 4D imaging can reveal biological processes that are difficult or impossible to capture using sequential z stack approaches, as well as highlighting new opportunities for physiological and functional imaging.

Imaging thick biological specimens, such as tissues, small animal models or 3D organoids, often forces researchers to compromise between achieving high subcellular resolution and capturing a large field of view (FOV). In this workshop we present the CrestOptics X-Light V3 Spinning Disk and DeepSIM Super-Resolution system, a correlative imaging platform that combines large-field imaging in confocal mode with a seamless transition to SIM super-resolution, all using shared optics and standard sample preparation. The X-Light V3 enables fast confocal acquisition of large 3D datasets with rapid deep Z-stacks and vignetting-free tile scans thanks to its extra-large FOV coupled with superior illumination flatness and enhanced sensitivity. The DeepSIM super-resolves intricate structures across samples of increasing morphological complexity and thickness, imaging at the same Z-depth of the Spinning Disk Confocal microscope. CrestOptics DeepSIM, indeed, offers full compatibility with the Low-to-Mid Magnification range, down to the 20x dry lens, where wider FOV, longer Working Distance and higher brightness of the Low-to-Mid Mag objectives are essential features for approaching thick biological samples. We will demonstrate the capabilities of this integrated workflow for high-content analysis of small animal models, organoids and tissues, with real case examples on a number of thick samples commonly imaged in any imaging facility.

Join us for the global launch of Cell Xtreme, the first microscope-integrated, label-free imaging system combining next-generation Structured Illumination Microscopy (SIM) with advanced Optical Diffraction Tomography (ODT).

In this workshop, we will introduce our innovative dual-modality platform, featuring the seamless integration of our leading technology MI-SIM and label-free MI-ODT for live-cell imaging. We will demonstrate how ODT provides comprehensive morphological context, enabling the identification of multiple subcellular structures without the need for labeling. Complementing this, SIM validates these structures and delivers super-resolution in live cell samples. Both imaging modalities are fully integrated and controlled through our advanced acquisition software IMAGER and supported by our dedicated data processing platform FINER, enabling streamlined workflows and high-quality quantitative analysis.

Live demonstrations of Cell Xtreme will showcase its unique capabilities in real-time imaging of living cells, highlighting the power of truly integrated, label-free multimodal microscopy.

This EVIDENT workshop presents the FLUOVIEW FV5000 confocal system on the IX85 platform, with a focus on workflow automation, reproducibility, and quantitative imaging enabled by the FLUOVIEW SMART AI guided software interface and integrated performance monitoring tools.

The session introduces how FLUOVIEW SMART streamlines the imaging process by automating key setup steps, including sample detection, focus alignment, and laser power optimization. By removing manual adjustment layers, the system reduces user-dependent variability and enables consistent imaging outcomes across users, experiments, and sites.

A dedicated part of the workshop covers the Microscope Performance Monitor, demonstrating how system performance can be tracked, validated, and documented over time especially in a muti-user environment In addition to our Laser Power Monitor and and SilVIR detector, this enables reproducible imaging conditions and supports long-term data comparability and truly quantitative imaging experiments

The workshop further highlights how the FV5000 supports reliable, quantitative fluorescence imaging through controlled photon detection and a wide dynamic range. This allows users to move from relative intensity measurements toward more robust and reviewable data, addressing increasing demands for reproducibility and data integrity in advanced imaging workflows.

PlantScope is a turnkey live-cell imaging platform designed for long-term observation of growing plant roots with automated tracking. The system enables researchers to follow root tip dynamics over hours to days while maintaining plants in a physiologically relevant upright growth orientation using a unique vertical stage design.

A key capability of PlantScope is automated live root tip tracking. As roots grow and change direction, the system continuously follows the advancing tip and acquires high-resolution image montages that capture the entire developmental cycle, including the meristematic, elongation, and differentiation zones at every time point. This enables comprehensive visualization of root growth dynamics, cell division patterns, tropic responses, and developmental trajectories across extended time-lapse experiments.

At the core of PlantScope, SlideBook and the Yokogawa CSU-W1 spinning disk confocal enable robust capture of dynamic biological processes in realtime. The CSU-W1 delivers rapid acquisition speeds, high sensitivity and multi-color sub-cellular imaging optimized for live samples. SlideBook software seamlessly coordinates all hardware – including microscope, camera, stage, spinning disk, laser launch, NIR lamp – in combination with automated tracking and adaptive imaging workflows in a familiar, easy-to-use interface.

In this workshop, we will demonstrate the PlantScope Marianas multimodal microscopy system with root tip tracking capabilities, showcasing automated long-term imaging workflows and high-resolution visualization of dynamic root development.

S. Untucht, P. Rodriguez, M. Beljan, K. Ritschel, A. Wede, A. Schmitz, B. Deissler, Z. Jiang, V. Augustin, J. Pearson, S. Schicktanz, F. Fahrbach, J. Reddington

Leica Microsystems CMS GmbH, Mannheim, Germany.

Here we present the new Leica Viventis SCAPE, based on oblique plane microscopy (OPM)¹ and swept confocally aligned planar excitation (SCAPE)²,³ technologies.

This system is designed for high-speed, high-throughput volumetric light sheet imaging and is compatible with commonly used carriers, including glass slides, Petri dishes, and well plates. Viventis SCAPE enables fast 3D imaging without the need for specialized holders or deviations from common sample mounting protocols.

We demonstrate the intuitively designed Viventis SCAPE workflow, from straightforward sample mounting in standard carriers and streamlined software integration to fast volumetric acquisition. Practical examples will illustrate how Viventis SCAPE enables high-speed 3D imaging of a wide range of specimens, including 2D cell culture, 3D tissues, organoids, and small organisms, providing new opportunities for observing biological dynamics in real time.

Join this session to discover how Viventis SCAPE delivers fast, gentle, and accessible light sheet imaging—bringing high-speed 3D microscopy into standard laboratory workflows.

1. Dunsby, C. et al. Optically sectioned imaging by oblique plane microscopy. Opt. Express 16, 20306–20316 (2008).

2. Bouchard, M. B. et al. Swept confocally-aligned planar excitation (SCAPE) microscopy for high-speed volumetric imaging of behaving organisms. Nat. Photonics 9, 113–119 (2015).

3. Voleti, V. et al. Real-time volumetric microscopy of in vivo dynamics and large-scale samples with SCAPE 2.0. Nat. Methods 16, 1054–1062 (2019).

Visualizing the three-dimensional architecture of complex biological specimens has traditionally required labor-intensive workflows and imaging times ranging from hours to days. To overcome these limitations, Miltenyi Biotec offers an integrated 3D imaging ecosystem that streamlines sample processing, acquisition, visualization, and analysis. In this workshop, we will present live demonstrations of the UltraMicroscope Blaze™ and its latest innovations for high-speed, scalable light sheet imaging. Attendees will experience the new LightSpeed mode, enabling up to 60-fold faster image acquisition for large-volume specimens, alongside the MACS iQ View – 3D Large Volume package for efficient visualization and handling of large multidimensional datasets with OCTAGON PRIME. We will further highlight an integrated 3D/2D workflow combining volumetric imaging with high-plex spatial analysis, providing a comprehensive solution for accelerated, data-rich biological discovery.

Steep learning curves, inconsistent image quality, and long scanning times are common challenges in slide scanning workflows. The NIS-Elements Slide Scanning solution overcomes them with an intuitive, guided workflow and pre optimized settings that eliminate the need for extensive training. High quality optics and AI powered focusing ensure sharp, reliable images while automatically identifying and capturing all regions of interest at optimal settings. High speed scanning and precise tiling reduce whole tissue acquisition to under a minute, and a streamlined gallery view enables easy visualization and downstream analysis directly within NIS Elements.

The NIS Elements Slide Scanning module is a powerful software solution in combination with the reliable Ni E upright microscope offers:

• Scanning of up to 8 standard or 4 double slides simultaneously

• Full automation combined with premium grade optics

• High resolution, high speed imaging in both brightfield and fluorescence

• An intuitive, user friendly interface

During the workshop, the complete workflow of the scanner will be demonstrated alongside practical examples showing how acquired images can be further enhanced using the powerful post processing and analysis tools available within the NIS Elements software.

Explore the future of slide scanning by registering for the upcoming workshop and discover how this innovative solution can enhance research workflows and laboratory efficiency.

Reserve a spot today and take the next step in advancing microscopy capabilities with Nikon.

The BC43 Benchtop Spinning Disk Confocal System, developed by Oxford Instruments, is designed to address the critical challenges of high-containment Biosafety BSL-2+/3/4 laboratories. This compact, high-performance system is validated for extreme fumigation protocols, ensuring safe decontamination and uninterrupted operation without voiding warranty. Its lightweight, light-tight benchtop design eliminates the need for dedicated darkrooms or additional infrastructure, making it ideal for restricted laboratory spaces.

Key Innovations

A key innovation of the BC43 is its comprehensive service support package, specifically tailored for BSL-2+/3/4 environments where external service visits are often impractical due to containment constraints. The system includes a dedicated BSL service solution that provides:

• Spare parts to enable rapid on-site repairs, minimizing downtime

• Remote support tools to troubleshoot issues in real-time, reducing the need for physical intervention and ensuring compliance with containment protocols.

• Certified training programs for customers, empowering facility staff to perform routine maintenance and servicing under PPE constraints.

• By integrating performance, containment adaptability, and proactive service support, the BC43 delivers a fully autonomous imaging solution for high-containment research, eliminating service gaps and maintaining workflow integrity.

A key innovation of the BC43 is its support for defined fumigation workflows, enabling routine full system decontamination while maintaining system integrity.

The Fusion software, equipped with guided protocols, facilitates fast and reliable data acquisition even under PPE constraints.

By combining performance, containment adaptability, and ease of use, the BC43 provides a robust solution for advanced bio-imaging in high-containment research facilities.

Using ONI’s Nanoimager with the Discovery KitTM: dSTORM in cells, the ultimate kit to prepare your samples for super-resolution imaging.

The Nanoimager is a compact and state-of-the-art microscope, offering quantitative analysis for localisation-based imaging (dSTORM, PALM, and DNA-PAINT), single-particle tracking, and single-molecule FRET. Designed to operate on any lab bench and with a footprint smaller than a piece of A4 paper, it simplifies super-resolution imaging.

The ONI Discovery Kit™: dSTORM in cells 2 provides a modular workflow for immunofluorescent labelling in cultured cells, which allows you to confidently detect extra and intracellular proteins in two channels with 20 nm resolution and high sensitivity in your own samples. You provide the cells and custom antibodies; we provide the rest!

This workshop is intended for scientists who are looking to brush up on their knowledge of dSTORM and to apply super-resolution to further their research at the molecular level.

Light-sheet microscopy is often approached from a system perspective, while in practice imaging results are primarily determined by the sample itself.

This workshop presents a sample-first approach, focusing on how sample geometry, mounting, and imaging conditions influence the final image quality. Through simple and practical demonstrations, we illustrate how light-sheet imaging can be performed using a minimal and robust setup, integrated with an existing microscope.

Participants will see how different sample configurations, including mounting in air or in medium, affect image quality and reproducibility. The workshop highlights how straightforward workflows can be used to validate samples, optimize preparation, and obtain reliable imaging results without introducing unnecessary system complexity.

This approach is particularly relevant for laboratories exploring light-sheet microscopy for the first time, as well as for facilities evaluating whether this technique is suitable for their applications. Emphasis is placed on accessibility, reproducibility, and practical decision-making in real experimental conditions.

The session concludes with an open discussion, where participants are encouraged to present their own samples and challenges for further exchange.

Quantitative time-resolved fluorescence techniques like Fluorescence Lifetime Imaging (FLIM) have become more attractive recently to study mechanisms driven by phase separation or to sense the cellular environment, for example.

PicoQuant`s innovative confocal microscope Luminosa combines state-of-the-art hardware with cutting edge software to deliver high quality data while simplifying daily operation. The software includes several features which improve the ease of use and reproducibility of experiments, including context-based workflows, sample-free auto-alignment and excitation laser power calibration. Still, if required for new method development every optomechanical component can be fully accessible.

We will show how FLIM is streamlined with Luminosa.. In combination with GPU-accelerated algorithms, this enables high-speed automated analysis of FLIM images. The InstaFLIM analysis workflow suggests the best fitting model based on statistical arguments, requiring minimal user interaction. The additional NovaFLIM software (also available for FLIM images acquired with LSM Upgrade kits) enables more extensive and advanced image analysis offering an holistic FLIM analysis process including exponential decay analysis, phasor plots and pattern matching.

The design of Luminosa`s software makes all data easily accessible. It works with the open, well documented PTU data format, enabling custom analysis while in includes enhanced, well structured metadata and ome-tiff exports.

Holotomography (HT) is a powerful tool for label-free, quantitative 3D imaging of living cells. By reconstructing the three-dimensional refractive index (RI) distribution of biological specimens, HT reveals subcellular structures—including nuclei, mitochondria, and lipid droplets—without the need for exogenous labels or staining, preserving the native physiological state of cells throughout the experiment.

In this workshop, we introduce the HT-X1 mini, the latest addition to Tomocube’s HT-X1 series. The HT-X1 mini brings full HT performance to an affordable, everyday-ready platform designed to lower the barrier to high-quality label-free imaging. As a table-top system, it fits seamlessly into culture rooms and space-limited environments, and operates via a laptop-based workflow with minimal setup requirements.

Despite its compact form factor, the HT-X1 mini delivers comparable performance to other HT-X1 series systems, supporting high-resolution 3D structural visualization and quantitative RI-based analysis through TomoAnalysis™ workflows. The system is expandable with optional modules—including a stage-top incubator for long-term live-cell imaging, a fluorescence light engine for target-specific signals, additional HT wavelengths, and a laser-based autofocus sensor—making it adaptable as research needs evolve.

This workshop will present representative applications enabled by the HT-X1 mini, including cell morphology observation, quantitative phenotyping, and routine live-cell culture monitoring. We will demonstrate how RI-based imaging provides immediate biological insight—from single-cell morphodynamics to subcellular organelle tracking—enabling rich structural insight from label-free RI data. Overall, we aim to show that HT is no longer a specialized capability reserved for a few labs, but an accessible, everyday imaging modality available to any cell biology researcher.

Super-resolution microscopy has made possible the exploration of biological systems at the nanoscale. One historical technique, STORM, uses a redox buffer to intermittently switch the fluorophores into a dark state to illuminate only a subset of molecules at a time and allows Single-Molecule Localization Microscopy (SMLM). Each blinking event is fitted to localize the position of the emitter with a 10 – 20 nm spatial resolution, enabling the visualization of cellular structures and molecular organization at the nanoscale. In the past, STORM was generally conducted on a single target because the use of different fluorophores introduced chromatic aberrations and complexity in the design of imaging buffer composition.

In this workshop, we demonstrate Abbelight multiplexed STORM imaging, enabled by the SAFe platform workflow, which features Spectral Demixing, an advanced multi-color detection design. By imaging far-red dyes with small differences in emission spectra, Spectral Demixing allows the simultaneous imaging of up to 3 targets with a single imaging buffer and produces aberration-free colocalization analysis. In Abbelight NEO LiveImagingTM software, users have access to real time demixing of the targets, allowing them to decide early on if a FOV is worth the full acquisition and saving precious time on the instrument. Abbelight NEO AnalysisTM software integrates a bias-free demixing algorithm, giving the option to the user to choose between high data recovery, optimal demixing and minimal crosstalk. The result: a streamlined and accelerated imaging strategy that reduces acquisition time, avoids chromatic abberrations and maintains the highest colocalization accuracy with minimal crosstalk.

Modern fluorescence microscopy applications need to be performed in natural environments of the structures of interests. This requires using large samples, like tissue slices, organoids/spheroids or even whole model organisms. One of the main limitations of Single Molecule Localization Microscopy microscopes is their weak ability to image structures deeper than few nm of the coverslip.

Engineered with simplicity and functionality in mind, the Bruker Vutara VXL system is different, as it uses robust widefield illumination in combination with the patented Biplane detection for 3D imaging. With this combination entire volumes in up to 50 µm depth inside the sample can be resolved in 3D with 20 nm resolution. The comprehensive processing and analysis pipeline in the SRX software package provides insights into the 3D-distribution of the detected molecules within cells in their native environment.

For decades, the pursuit of sub-diffraction resolution has forced researchers into uncomfortable trade-offs: resolution gained at the cost of phototoxicity, imaging speed sacrificed for optical sectioning quality, or biological relevance compromised by sample preparation demands. ZEISS Lattice SIM 5 was designed from the ground up to resolve these conflicts.

At the core of the system lies a fundamentally different approach to structured illumination. Rather than projecting conventional grid patterns across a single plane, Lattice SIM generates an optimized three-dimensional lattice of light spots that structures illumination throughout the full depth of the sample. This produces sharper interference patterns, dramatically higher contrast, and half the light dose of classic SIM for the same information content. The SIM² image reconstruction algorithm then transforms the acquired data into strikingly crisp, super-resolved images — in both fixed and living specimens.

In this hands-on workshop session, participants will explore the full versatility of the platform — from resolving subcellular architecture at high resolution to imaging dynamics in living cells and optically sectioning thick 3D models. We will also discuss how to match imaging mode to biological question, and demonstrate how the ZEN software environment integrates acquisition, reconstruction analysis into a single streamlined workflow.

Mechanobiology is an emerging field in life science, and the role of mechanical cues has gained relevance in the understanding of the physical state and function of biological systems. However, current gold standard techniques to measure biomechanical properties are invasive, mainly contact-based and limited to surface probing, they lack 3D sub-cellular resolution capabilities or they are not always suitable for multicellular environments.

Confocal Brillouin microscopy has recently emerged as an all-optical, contact- and label-free technique for in vivo 3D subcellular mapping of elasticity and viscosity distribution within cells and tissues, even in settings where physical contact is not possible, providing sufficient sensitivity to detect and monitor relevant biomechanical changes. In this talk, we will walk you through the technological challenges and advancements of the technique, showing representative examples of real biological experiments that highlight the potential of this technique, especially when integrated in an advanced spinning disk correlative confocal imaging platform

Modern microscopy is no longer defined by a single technique. Each modality brings unique strengths and limitations, and it is the combination of these approaches that unlocks the full potential of biological imaging. To truly maximize information from complex samples, multi-modal integration has become essential especially in super-resolution imaging. However, current workflows are often limited by fragmented software, inefficient modality switching, and challenging cross-modality data integration.

CSR Biotech’s multi-modal super-resolution solutions overcome these challenges, enabling seamless integration of Structured Illumination Microscopy (SIM), Spinning Disk and label-free Optical Diffraction Tomography (ODT) imaging modalities. In this workshop, we will focus on dual-modality acquisitions with hands-on SIM and ODT, unified and automated workflow tools, intelligent image registration, and event-based triggering for smart microscopy to simplify complex experiments in multi-modal imaging.

Finally, we will introduce the FINER multi-modal image processing suite, which includes tools for resolution enhancement such as SIM and SACD, AI-assisted denoising and segmentation.

This EVIDENT workshop introduces the FLUOVIEW FV5000MPE platform on the IX85 inverted microscope, focusing on compact and convenient multiphoton integration for deep tissue imaging applications. We will present to you the easy integration of pig tailed fibre lasers on top of a confocal imaging platform to achieve a multiphoton ready combo system. The session highlights how this design reduces system complexity and footprint while maintaining high-performance imaging capabilities. Attendees will understand how fiber-based laser delivery supports more robust alignment, easier maintenance, and improved day-to-day usability, making multiphoton imaging more accessible in shared environments.

A dedicated part of the workshop covers SilVIR non-descanned detectors (NDD), designed for high-sensitivity photon detection in scattering specimens. Optimized detection geometry combined with photon-counting capabilities improves signal collection efficiency at depth while maintaining stable and quantitative imaging conditions.

Together, these elements position the FV5000MPE as a solution that brings multiphoton imaging closer to routine use, balancing deep imaging performance with operational simplicity and system stability within the EVIDENT platform.

PlantScope is a turnkey live-cell imaging platform designed for long-term observation of growing plant roots with automated tracking. The system enables researchers to follow root tip dynamics over hours to days while maintaining plants in a physiologically relevant upright growth orientation using a unique vertical stage design.

A key capability of PlantScope is automated live root tip tracking. As roots grow and change direction, the system continuously follows the advancing tip and acquires high-resolution image montages that capture the entire developmental cycle, including the meristematic, elongation, and differentiation zones at every time point. This enables comprehensive visualization of root growth dynamics, cell division patterns, tropic responses, and developmental trajectories across extended time-lapse experiments.

At the core of PlantScope, SlideBook and the Yokogawa CSU-W1 spinning disk confocal enable robust capture of dynamic biological processes in realtime. The CSU-W1 delivers rapid acquisition speeds, high sensitivity and multi-color sub-cellular imaging optimized for live samples. SlideBook software seamlessly coordinates all hardware – including microscope, camera, stage, spinning disk, laser launch, NIR lamp – in combination with automated tracking and adaptive imaging workflows in a familiar, easy-to-use interface.

In this workshop, we will demonstrate the PlantScope Marianas multimodal microscopy system with root tip tracking capabilities, showcasing automated long-term imaging workflows and high-resolution visualization of dynamic root development.

S. Untucht, P. Rodriguez, M. Beljan, K. Ritschel, A. Wede, A. Schmitz, B. Deissler, Z. Jiang, V. Augustin, J. Pearson, S. Schicktanz, F. Fahrbach, J. Reddington

Leica Microsystems CMS GmbH, Mannheim, Germany.

Here we present the new Leica Viventis SCAPE, based on oblique plane microscopy (OPM)¹ and swept confocally aligned planar excitation (SCAPE)²,³ technologies.

This system is designed for high-speed, high-throughput volumetric light sheet imaging and is compatible with commonly used carriers, including glass slides, Petri dishes, and well plates. Viventis SCAPE enables fast 3D imaging without the need for specialized holders or deviations from common sample mounting protocols.

We demonstrate the intuitively designed Viventis SCAPE workflow, from straightforward sample mounting in standard carriers and streamlined software integration to fast volumetric acquisition. Practical examples will illustrate how Viventis SCAPE enables high-speed 3D imaging of a wide range of specimens, including 2D cell culture, 3D tissues, organoids, and small organisms, providing new opportunities for observing biological dynamics in real time.

Join this session to discover how Viventis SCAPE delivers fast, gentle, and accessible light sheet imaging—bringing high-speed 3D microscopy into standard laboratory workflows.

1. Dunsby, C. et al. Optically sectioned imaging by oblique plane microscopy. Opt. Express 16, 20306–20316 (2008).

2. Bouchard, M. B. et al. Swept confocally-aligned planar excitation (SCAPE) microscopy for high-speed volumetric imaging of behaving organisms. Nat. Photonics 9, 113–119 (2015).

3. Voleti, V. et al. Real-time volumetric microscopy of in vivo dynamics and large-scale samples with SCAPE 2.0. Nat. Methods 16, 1054–1062 (2019).

Visualizing the three-dimensional architecture of complex biological specimens has traditionally required labor-intensive workflows and imaging times ranging from hours to days. To overcome these limitations, Miltenyi Biotec offers an integrated 3D imaging ecosystem that streamlines sample processing, acquisition, visualization, and analysis. In this workshop, we will present live demonstrations of the UltraMicroscope Blaze™ and its latest innovations for high-speed, scalable light sheet imaging. Attendees will experience the new LightSpeed mode, enabling up to 60-fold faster image acquisition for large-volume specimens, alongside the MACS iQ View – 3D Large Volume package for efficient visualization and handling of large multidimensional datasets with OCTAGON PRIME. We will further highlight an integrated 3D/2D workflow combining volumetric imaging with high-plex spatial analysis, providing a comprehensive solution for accelerated, data-rich biological discovery.

3D microscopy has advanced rapidly with spheroid and organoid models, which closely replicate native tissue. While acquiring quality 3D images is challenging, developing robust and reproducible analysis workflows can be even more difficult, often requiring Python expertise and limiting accessibility. NIS Elements addresses this with an intuitive, visual programming interface, allowing users to drag and drop functions for 2D/3D processing, measurement, plotting, and data management—eliminating the need for scripting while maintaining flexibility.

For specialized analyses, users can incorporate open‑source algorithms via integrated Python scripting and LLM prompts, enabling easy access to tools like Cellpose3, Cellpose SAM, Stardist, and others. If these pretrained models lack desired performance, NIS Elements supports custom 3D deep learning training for segmentation, signal enhancement, and channel prediction, using user data for higher accuracy and speed. To handle computational demands from large datasets, Cluster Computing software distributes tasks across idle lab computers.

In this workshop, we will show how to:

• Use visual programming for 3D image analysis workflows

• Make use of pretrained open-source segmentation models inside NIS-Elements

• Create 3D binaries with the help of detect.ai and train custom 3D segmentation model

• Use LLM to make a Python script to be used inside NIS-Elements

• Run Batch Analysis using Cluster Computing on Octagon PRIME – high performance processing unit from Octagon Data GmbH

Finally, you can use your 3D analysis pipeline as part of acquisition to enable feedback driven microscopy workflows, removing the need for users to remain physically present at the microscope.

Modern microscopy produces increasingly large and complex image datasets, yet image analysis often remains repetitive, time-consuming, and difficult to reproduce. Researchers frequently apply identical analysis steps across multiple images while manually documenting parameters and processing decisions, creating challenges for efficiency, reproducibility, and collaboration.

This workshop introduces the Image Analysis Workflows available in Imaris 11 and demonstrates how they can automate repetitive tasks, standardize analysis pipelines, and capture the full analytical process in a transparent, shareable format. Participants will learn how workflows record preprocessing, object creation, filtering, masking, quantification, visualization, and other analysis steps while generating reusable workflow files that can be exported and shared with collaborators.

The session will also highlight the new workflow resources introduced in Imaris 11.1, including application-specific workflows developed by the Imaris team to simplify common analysis tasks and accelerate user training. Attendees will explore practical examples designed to reduce the learning curve and improve analytical consistency.

In addition, the workshop will cover AI-driven segmentation and object classification tools integrated into Imaris workflows. Participants will learn how machine learning–based segmentation can be combined with preprocessing methods such as deconvolution, as well as advanced analysis modules including cell detection, object tracking, and filament or blood vessel tracing.

Finally, attendees will discover how validated workflows can be applied to many similar datasets using batch processing, enabling efficient, reproducible analysis while still allowing expert-driven manual input when required.

A hands-on introduction to DNA-PAINT imaging, from experimental design to structures redefined.

DNA-PAINT is a Single-Molecule Localization Microscopy (SMLM) technique that enables the precise visualization of structures with high-resolution through transient DNA binding events.

This workshop will cover the full ONI DNA-PAINT workflow: from experimental design and sample preparation to image acquisition and quantitative analysis using CODI. The focus is on building a practical understanding of how to design robust experiments, acquire high-quality datasets, and extract quantitative information from nanoscale biological structures.

This hands-on session is ideal for researchers looking to get started with DNA-PAINT and develop confidence in end-to-end super-resolution imaging workflows.

Light-sheet microscopy is often approached from a system perspective, while in practice imaging results are primarily determined by the sample itself.

This workshop presents a sample-first approach, focusing on how sample geometry, mounting, and imaging conditions influence the final image quality. Through simple and practical demonstrations, we illustrate how light-sheet imaging can be performed using a minimal and robust setup, integrated with an existing microscope.

Participants will see how different sample configurations, including mounting in air or in medium, affect image quality and reproducibility. The workshop highlights how straightforward workflows can be used to validate samples, optimize preparation, and obtain reliable imaging results without introducing unnecessary system complexity.

This approach is particularly relevant for laboratories exploring light-sheet microscopy for the first time, as well as for facilities evaluating whether this technique is suitable for their applications. Emphasis is placed on accessibility, reproducibility, and practical decision-making in real experimental conditions.

The session concludes with an open discussion, where participants are encouraged to present their own samples and challenges for further exchange.

Recently, high-performance SPAD-arrays featuring few tens of pixels have become available. Combining these with suitable multi-channel TCSPC-devices enables time-resolved Image Scanning Microscopy (ISM). ISM enhances the spatial resolution and increases image contrast compared to standard confocal imaging. FLIM can provide additional functional information as well as extended marker multiplexing using lifetime contrast. So both technologies complement each other.

In this workshop we will show how the PDA23 SPAD Array is implemented in Luminosa. The newly released software NovaISM enables the analysis of ISM-FLIM images acquired with the PDA-23 Add-On of the Luminosa microscope. Image scanning microscopy (ISM) with a SPAD array detector achieves resolution enhancements of about 1.5 to 1.7 times in comparison to standard confocal images, in combination with deconvolution. Even for 2d-recordings/data the contrast of the ISM-FLIM images is enhanced significantly by rejecting the out-of focus light. Such rejection enhances not only the signal-to-noise-ratio, but also the lifetime contrast of the FLIM images. These benefits enable either faster image acquisition or gentler imaging of live samples.

Holotomography (HT) is a powerful tool for label-free, quantitative 3D imaging of living cells. By reconstructing the three-dimensional refractive index (RI) distribution of biological specimens, HT reveals subcellular structures—including nuclei, mitochondria, and lipid droplets—without the need for exogenous labels or staining, preserving the native physiological state of cells throughout the experiment.

In this workshop, we introduce the HT-X1 mini, the latest addition to Tomocube’s HT-X1 series. The HT-X1 mini brings full HT performance to an affordable, everyday-ready platform designed to lower the barrier to high-quality label-free imaging. As a table-top system, it fits seamlessly into culture rooms and space-limited environments, and operates via a laptop-based workflow with minimal setup requirements.

Despite its compact form factor, the HT-X1 mini delivers comparable performance to other HT-X1 series systems, supporting high-resolution 3D structural visualization and quantitative RI-based analysis through TomoAnalysis™ workflows. The system is expandable with optional modules—including a stage-top incubator for long-term live-cell imaging, a fluorescence light engine for target-specific signals, additional HT wavelengths, and a laser-based autofocus sensor—making it adaptable as research needs evolve.

This workshop will present representative applications enabled by the HT-X1 mini, including cell morphology observation, quantitative phenotyping, and routine live-cell culture monitoring. We will demonstrate how RI-based imaging provides immediate biological insight—from single-cell morphodynamics to subcellular organelle tracking—enabling rich structural insight from label-free RI data. Overall, we aim to show that HT is no longer a specialized capability reserved for a few labs, but an accessible, everyday imaging modality available to any cell biology researcher.

Super-resolution microscopy has transformed the way we explore biological systems. One popular technique, DNA-PAINT, exploits the intermittent binding of fluorescent DNA to their targets to illuminate only a subset of molecules at a time and allows Single-Molecule Localization Microscopy (SMLM). Each blinking event is fitted to localize the position of the emitter with a 10 – 20 nm spatial resolution, enabling the visualization of cellular structures and molecular organization at the nanoscale. In the past, DNA-PAINT generally suffered from small fields of view (FOV) and lengthy acquisitions, limiting the overall throughput of data collection.

In this workshop, we demonstrate Abbelight high-throughput DNA-PAINT imaging, enabled by the SAFe platform workflow, which features ASTER illumination, a proprietary flat field excitation technology. Our system enables an ultra-wide 230 x 230 µm fields-of-view and the illumination power is 95% homogeneous across the whole FOV ensuring consistent fluorophore excitation from center to edge and reliable quantifications. In Abbelight NEO LiveImagingTM software, users have access to real-time processing of the data, allowing them to decide early on if a FOV is worth the full acquisition and saving precious time on the instrument. By programming automated acquisitions, the Multi-Dimensional Acquisition (MDA) enables stitching of millimeter-sized regions, plate screening and target multiplexing using the Smartflow microfluidic system to conduct exchange-PAINT. In Abbelight NEO AnalysisTM software, the large datasets can be batched-processed overnight and favor time optimization. The result: higher statistical power and data-rich acquisitions that open the door to population level insights and rare event detection.

Modern, high throughput 3D microscopy not only requires solutions for rapid image acquisition, but also for scalable, on-the-fly image data transfer, image processing and content analysis. This workshop presents an integrated imaging and computing workflow combining the Bruker Luxendo light-sheet microscope with the Acquifer HIVE data management, a multi-user, high performance computing platform.

Multi-camera, multi-view light sheet microscopes enable fast volumetric imaging of complex 3D biological samples such as spheroids, organoids, and developing organisms, delivering high optical efficiency and gentle illumination across multiple imaging dimensions. To match this acquisition speed, imaging data is directly streamed to Acquifer HIVE, where it is centrally stored, processed, and made immediately available for analysis and word-wide collaboration, all in an integrated workflow made available by smart Hardware and Software implementation. The workshop demonstrates how end to end optimization—from image acquisition to compute accelerated processing—removes data bottlenecks and enables efficient, reproducible 3D microscopy workflows for microscopists, imaging facilities and translational research.

ZEN software from ZEISS provides multiple tools to approach Smart Microscopy. These tools will be demonstrated in two separate workshops. The first workshop is designed for users without scripting experience and will focus on GUI-based procedures for typical event- or phenotype-driven high-resolution imaging using the Guided Acquisition. The second workshop targets advanced coders and will highlight custom Smart Imaging techniques utilizing the ZEN-API.

Advanced imaging techniques have become crucial tools in biomedical research, enabling scientists to explore and document intricate biological structures and their dynamics across scales, from single biomolecules to entire animals.

Image acquisition workflows include positioning of the sample inside the microscope, finding the imaging target and setting the acquisition parameters. Traditionally, these routine steps are often performed manually. While manual operation gives experimenters control over the acquisition process, this limits applicability and scalability.

Smart Imaging approaches circumvent these limitations, by combining imaging with on-the-fly processing of acquired data to trigger changes in the imaging settings. Using live data to steer the acquisition or sample perturbation broadens the capabilities of microscopes to new types of experiments. For example, high-resolution imaging of a specific (rare) phenotypes becomes accessible when identifying the object of interest in a low-resolution overview beforehand, followed by switching to a high-resolution imaging modality only for the relevant field-of-view determined by image analysis.

This workshop presents the EVIDENT IXplore IX85 SpinXL system, combining high-speed spinning disk confocal imaging with an expanded field of view enabled by FN26.5 optics. Built on the IX85 platform, and powered by CrestOptics spinning disk technology the system is designed to capture more biologically relevant context per frame, reducing the need for stitching while increasing experimental throughput.

The workshop focuses on how large field-of-view imaging directly impacts workflow efficiency in live-cell and high-content applications. By acquiring larger areas in a single shot, users can monitor more cells simultaneously, improve statistical relevance, and reduce acquisition time. The spinning disk architecture further supports gentle imaging conditions, minimizing phototoxicity and enabling longer time-lapse experiments.

Participants will explore how the system balances speed, sensitivity, and scalability, making it suitable for shared environments such as imaging core facilities. Emphasis is placed on consistent data generation across users, simplified operation, and adaptability to different experimental needs.

Structured Illumination Microscopy (SIM) has become a key approach for super-resolution imaging. However, classical SIM and conventional systems are often limited by trade-offs in speed, resolution, photobleaching control, and workflow flexibility, particularly in demanding live and volumetric imaging applications.

This workshop presents CSR Biotech’s next-generation MI-SIM platform, addressing these limitations through advanced hardware–software integration. During the session, we will demonstrate high-speed and high-resolution live-cell imaging and explore the power of real-time reconstruction. We will also highlight how Sparse deconvolution allows for the extended imaging of sensitive samples without sacrificing structural detail.

We will further examine the platform’s multiple imaging modes, including TIRF-SIM and 3D-SIM. Finally, we will showcase smart imaging features such as AI-based cell division detection and cell tracking for time-series analysis. These advancements illustrate a highly automated and intelligent SIM imaging workflow.

Understanding how molecular expression patterns relate to tissue structure and cellular context remains a central challenge in spatial biology. In this context, multiplexed imaging is particularly powerful, as it allows multiple biomarkers to be analysed simultaneously, providing deeper insight into complex biological processes within a single sample.

The latest development of the SLIDEVIEW VS200 slide scanner is specifically designed to support spatial biology workflows by enabling the acquisition of up to 10 fluorescence channels in a single imaging round. By reducing repeated staining cycles and slide handling, this approach minimizes tissue stress, improves data consistency, and helps preserve sample integrity. In addition, the use of dedicated filter sets eliminates the need for spectral unmixing, resulting in more straightforward downstream analysis and significantly reduced processing time.

Beyond advanced fluorescence capabilities, SLIDEVIEW VS200 combines large area, high throughput slide scanning with true multimodal imaging, including brightfield, polarized light, transmitted light, Structured illumination (VS-SILA) and fluorescence acquisition. This multimodality allows users to generate comprehensive datasets that seamlessly integrate morphological and molecular information from the same tissue section.

PlantScope is a turnkey live-cell imaging platform designed for long-term observation of growing plant roots with automated tracking. The system enables researchers to follow root tip dynamics over hours to days while maintaining plants in a physiologically relevant upright growth orientation using a unique vertical stage design.

A key capability of PlantScope is automated live root tip tracking. As roots grow and change direction, the system continuously follows the advancing tip and acquires high-resolution image montages that capture the entire developmental cycle, including the meristematic, elongation, and differentiation zones at every time point. This enables comprehensive visualization of root growth dynamics, cell division patterns, tropic responses, and developmental trajectories across extended time-lapse experiments.

At the core of PlantScope, SlideBook and the Yokogawa CSU-W1 spinning disk confocal enable robust capture of dynamic biological processes in realtime. The CSU-W1 delivers rapid acquisition speeds, high sensitivity and multi-color sub-cellular imaging optimized for live samples. SlideBook software seamlessly coordinates all hardware – including microscope, camera, stage, spinning disk, laser launch, NIR lamp – in combination with automated tracking and adaptive imaging workflows in a familiar, easy-to-use interface.

In this workshop, we will demonstrate the PlantScope Marianas multimodal microscopy system with root tip tracking capabilities, showcasing automated long-term imaging workflows and high-resolution visualization of dynamic root development.

L.A.J. Alvarez, I. Steinmetz, U. Schwarz, V. Augustin, J. Pearson, H. Birk, F. Hecht, J. Roberti

Leica Microsystems CMS, Am Friedensplatz 3, 68165, Mannheim, Germany.

In this workshop, we present three advances for the Leica Microsystems STELLARIS platform: new detectors, automated correction collar optimization, and real-time AI denoising.

We introduce the new generation of multi-pixel photon counting (MCCP)-based Power HyD detectors, the new Power HyD S and Power HyD N. The Power HyD N is a far-red-shifted detector with enhanced photon detection efficiency in the near-infrared. Together with the updated Power HyD S, both feature improved electronics and cooling to −39 °C, reducing dark counts and enhancing single-photon discrimination. Combined with PowerCounting (1) and White Light Laser excitation up to 790 nm, they enable quantitative multiplexed imaging across visible and NIR spectra while retaining lifetime imaging capabilities.

SmartCORR automatically optimizes motorized correction collar settings to match sample-specific conditions, significantly improving image quality by reducing sample-dependent spherical aberrations caused by variations in coverslip thickness or specimen properties. This eliminates time-consuming manual adjustment and minimizes human bias and error. SmartCorr allows to dynamically make changes for larger samples with inhomogeneous properties in confocal, multiphoton and TauSTED experiments.

To boost image quality on-the-fly AI denoising framework, based on Noise2Noise (2) and trained on detector-specific data, enables real-time denoising during live scanning across all STELLARIS systems, featuring a more linearized response, temporal stabilization, and a hallucination blocker to prevent artefacts in photon-sparse regions with the original raw always preserved.

1. Schweikhard, V. et al. (2020). DOI: 10.1038/d42473-020-00398-0.

2. Lehtinen, J. et al. (2018).

Visualizing the three-dimensional architecture of complex biological specimens has traditionally required labor-intensive workflows and imaging times ranging from hours to days. To overcome these limitations, Miltenyi Biotec offers an integrated 3D imaging ecosystem that streamlines sample processing, acquisition, visualization, and analysis. In this workshop, we will present live demonstrations of the UltraMicroscope Blaze™ and its latest innovations for high-speed, scalable light sheet imaging. Attendees will experience the new LightSpeed mode, enabling up to 60-fold faster image acquisition for large-volume specimens, alongside the MACS iQ View – 3D Large Volume package for efficient visualization and handling of large multidimensional datasets with OCTAGON PRIME. We will further highlight an integrated 3D/2D workflow combining volumetric imaging with high-plex spatial analysis, providing a comprehensive solution for accelerated, data-rich biological discovery.

High-content screening and high-resolution confocal imaging have traditionally relied on separate, specialized setups. Imaging well plates at high resolution with reliable statistical output often involves complex hardware and software integration. The combination of Nikon’s ECLIPSE Ji and AX confocal microscope overcomes these challenges, enabling automatic acquisition of large sample sets at the highest resolution.

ECLIPSE Ji – Ease of Use and Robustness: Ji is an AI-powered acquisition and analysis platform that simplifies microscopy workflows and reduces experiment time. It automates setup steps to ensure reproducible, high-quality imaging with minimal intervention—ideal for users of all experience levels. Key capabilities include:

• Automated well plate detection, sample alignment, brightfield AI autofocus, and AutoSignal.ai for optimized exposure settings.

• Smart Experiments with pre-defined assay protocols for fully automated high-content screening.

• Custom Experiments for designing personalized imaging and analysis workflows with the same automation and reproducibility.

Nikon AX, NSPARC – Superior 3D Imaging: AX system with NSPARC detector delivers exceptional optical performance for diverse magnifications and sample types. Key features include:

• 25 mm field of view with Galvo (8K) and Resonant (2K) scanning for detailed tissue imaging and fast, gentle live-cell acquisition.

• Excellent optical sectioning for deep imaging of complex 3D samples such as organoids.

• High resolving power with simultaneous multicolor imaging of fine 3D structures.

During the workshop, participants will experience an end-to-end 3D spheroid imaging workflow—from automated detection and adaptive Z‑stacking to high‑resolution confocal imaging and downstream analysis—powered by NIS‑Elements JOBS and GA3 modules for smart feedback microscopy.

Confocal imaging has long been tied to large, complex systems—requiring darkrooms, extensive training, and ongoing specialist support. These barriers often slow down researcher independence and delay results and publications. On top of that, traditional high-end confocal platforms demand continuous, costly maintenance.

Oxford Instruments BC43 changes the game. In a footprint no larger than a printer, BC43 delivers powerful, high-quality confocal imaging—without the complexity of traditional systems. Built with a unique quality control program, every system is guaranteed to deliver consistent, reliable image quality.

Designed around real user needs, BC43 offers unmatched flexibility: start with widefield imaging and upgrade in the field to confocal or super-resolution as your needs evolve.

The new Fusion Benchtop software takes productivity even further, introducing advanced capabilities such as:

• Irregular and multiple irregular montage

• Focus maps for complex samples.

• High-performance large data acquisition

All within an intuitive, easy-to-use workflow.

In this workshop, you will learn how to:

1. Start imaging immediately — no special training required

2. Capture large 3D datasets quickly and effortlessly

3. Acquire high-quality 2D/3D data with seamless stitching

4. Boost productivity using the new powerful Fusion Benchtop features.

Join us to discover how BC43 delivers high-end confocal performance, outstanding image quality, and true ease of use—all in a compact benchtop system designed to accelerate your research.

Turning signals into breakthroughs with multi-target DNA-PAINT imaging to reveal subcellular complexity beyond the diffraction limit.

DNA-PAINT is a Single-Molecule Localisation Microscopy (SMLM) technique that enables the precise visualisation of structures with high-resolution through transient DNA binding events. By using DNA barcodes, it enables sequential multi-target imaging with high specificity and minimal spectral constraints.

The ONI DNA-PAINT Kit from Massive Photonics streamlines this workflow by providing two single-domain antibodies (sdABs) for DNA-PAINT, along with imager strands and optimized buffers for sample preparation and imaging. With two sdABs per primary antibody, the kit enables robust 2-color DNA-PAINT imaging on ONI microscopes by simply adding the primary antibodies and proceeding directly to imaging.

Participants in this session will learn how to design and execute multi-target DNA-PAINT experiments and acquire high-quality datasets on the ONI Nanoimager platform.

Light-sheet microscopy is often approached from a system perspective, while in practice imaging results are primarily determined by the sample itself.

This workshop presents a sample-first approach, focusing on how sample geometry, mounting, and imaging conditions influence the final image quality. Through simple and practical demonstrations, we illustrate how light-sheet imaging can be performed using a minimal and robust setup, integrated with an existing microscope.

Participants will see how different sample configurations, including mounting in air or in medium, affect image quality and reproducibility. The workshop highlights how straightforward workflows can be used to validate samples, optimize preparation, and obtain reliable imaging results without introducing unnecessary system complexity.

This approach is particularly relevant for laboratories exploring light-sheet microscopy for the first time, as well as for facilities evaluating whether this technique is suitable for their applications. Emphasis is placed on accessibility, reproducibility, and practical decision-making in real experimental conditions.

The session concludes with an open discussion, where participants are encouraged to present their own samples and challenges for further exchange.

“Smart” or Adaptive Feedback Microscopy or Event Driven Microscopy aims to combine bioimage analysis (and other algorithms) with fully motorized and computer-controlled microscopes to create automated and adaptive measurement sequences. This enables the user for example to monitor transient and rare events in the desired level of detail across a large cell population or over long observation times or to streamline the optimization of acquisition parameters and the batch analysis of multiple measurements in a single script.

Here, we present the development of a scripting concept based on the Python programming language for the fully motorized single-photon counting confocal microscope Luminosa. We illustrate these benefits by presenting specific use cases for automated optimization of acquisition parameters. The framework fully opens up the microscope interface to allow the user to call external python libraries without compromising the workflows and functionalities present in the microscope software, such as the user settings, laser safety interlock, and predefined functionality blocks for auto-alignment, live views, etc. Thus scripting demands for the user remain rather low enabling non-experts to develop their own measurement/analysis pipelines.

We envision that the community will build upon this python interface bringing the rapidly evolving open source activities closer to the commercial instrumentation allowing for more efficient method developments nd granting end users direct access to cutting-edge developments. As an example of all possibilities opened via the python interface a connection of between Luminosa and AI-Agents will be shown.

Holotomography (HT) is a powerful tool for label-free, quantitative 3D imaging of living cells. By reconstructing the three-dimensional refractive index (RI) distribution of biological specimens, HT reveals subcellular structures—including nuclei, mitochondria, and lipid droplets—without the need for exogenous labels or staining, preserving the native physiological state of cells throughout the experiment.

In this workshop, we introduce the HT-X1 mini, the latest addition to Tomocube’s HT-X1 series. The HT-X1 mini brings full HT performance to an affordable, everyday-ready platform designed to lower the barrier to high-quality label-free imaging. As a table-top system, it fits seamlessly into culture rooms and space-limited environments, and operates via a laptop-based workflow with minimal setup requirements.

Despite its compact form factor, the HT-X1 mini delivers comparable performance to other HT-X1 series systems, supporting high-resolution 3D structural visualization and quantitative RI-based analysis through TomoAnalysis™ workflows. The system is expandable with optional modules—including a stage-top incubator for long-term live-cell imaging, a fluorescence light engine for target-specific signals, additional HT wavelengths, and a laser-based autofocus sensor—making it adaptable as research needs evolve.

This workshop will present representative applications enabled by the HT-X1 mini, including cell morphology observation, quantitative phenotyping, and routine live-cell culture monitoring. We will demonstrate how RI-based imaging provides immediate biological insight—from single-cell morphodynamics to subcellular organelle tracking—enabling rich structural insight from label-free RI data. Overall, we aim to show that HT is no longer a specialized capability reserved for a few labs, but an accessible, everyday imaging modality available to any cell biology researcher.

Total Internal Reflection Fluorescence (TIRF) microscopy is a powerful tool for studying dynamic biological processes that occur at or near the plasma membrane or during in vitro reconstitutions. An evanescent field excites fluorophores in a thin slice of 100 to 200 nm above the glass surface, achieving a sectioning 5 to 10 times sharper than confocal microscopy. By minimizing background and achieving a high signal-to-noise ratio, dynamic processes can be monitored precisely and with low cytotoxicity. In the past, TIRF was generally conducted one color at a time, limiting the extent of interpretation when following complex mechanisms involving multiple molecular partners.

In this workshop, we demonstrate Abbelight simultaneous multicolor TIRF imaging, enabled by the SAFe platform workflow, designed to combine versatility, automation, and precision. Our system enables up to 4-colors imaging, both simultaneous and sequential, giving researchers the flexibility to study complex phenomenon involving multiple molecular partners or structures within the same field of view.

Based on automatic calibration, Abbelight NEO LiveImagingTM software predicts the penetration depth based on the illumination angle chosen by the user. This gives users precise control over the excitation depth and ensures reproducible imaging and quantifications across experimental sessions. Overall, Abbelight SAFe simultaneous multicolor TIRF imaging enables the investigation of protein colocalization, receptor clustering, membrane trafficking, phase separation, biocondensates dynamics, signal transduction, and many other dynamic membrane processes.

Modern fluorescence microscopy applications need to be performed in natural environments of the structures of interests. This requires using large samples, like tissue slices, organoids/spheroids or even whole model organisms. One of the main limitations of Single Molecule Localization Microscopy microscopes is their weak ability to image structures deeper than few nm of the coverslip.

Engineered with simplicity and functionality in mind, the Bruker Vutara VXL system is different, as it uses robust widefield illumination in combination with the patented Biplane detection for 3D imaging. With this combination entire volumes in up to 50 µm depth inside the sample can be resolved in 3D with 20 nm resolution. The comprehensive processing and analysis pipeline in the SRX software package provides insights into the 3D-distribution of the detected molecules within cells in their native environment.

Biological processes occur across spatial and temporal scales that increasingly challenge conventional three dimensional imaging approaches. Capturing fast physiological dynamics in living systems requires volumetric imaging methods that provide high temporal resolution without compromising spatial context. The recently introduced Lightfield 4D technology, integrated into the ZEISS LSM 910 and LSM 990 platforms, addresses this challenge by enabling instant volumetric acquisition within a single camera exposure.

Implemented as a modular component of the LSM 910 and LSM 990, ZEISS Lightfield 4D allows for mixed modality workflows where images of the sample specimen can be captured at high temporal resolution with the Lightfield and then LSM for higher spatial resolution, spectral multiplex imaging, molecular dynamics measurements, and photomanipulation. This flexibility supports experiments across a wide range of sample types, including cultured cells, organoids, tissue sections, and small model organisms.

In this presentation, we will introduce the principles underlying Lightfield 4D imaging on the ZEISS LSM 910 and LSM 990 platforms and discuss how instant volumetric acquisition reshapes experimental design for dynamic biological systems. Application examples will be shown to illustrate how high speed 4D imaging can reveal biological processes that are difficult or impossible to capture using sequential z stack approaches, as well as highlighting new opportunities for physiological and functional imaging.

Imaging thick biological specimens, such as tissues, small animal models or 3D organoids, often forces researchers to compromise between achieving high subcellular resolution and capturing a large field of view (FOV). In this workshop we present the CrestOptics X-Light V3 Spinning Disk and DeepSIM Super-Resolution system, a correlative imaging platform that combines large-field imaging in confocal mode with a seamless transition to SIM super-resolution, all using shared optics and standard sample preparation. The X-Light V3 enables fast confocal acquisition of large 3D datasets with rapid deep Z-stacks and vignetting-free tile scans thanks to its extra-large FOV coupled with superior illumination flatness and enhanced sensitivity. The DeepSIM super-resolves intricate structures across samples of increasing morphological complexity and thickness, imaging at the same Z-depth of the Spinning Disk Confocal microscope. CrestOptics DeepSIM, indeed, offers full compatibility with the Low-to-Mid Magnification range, down to the 20x dry lens, where wider FOV, longer Working Distance and higher brightness of the Low-to-Mid Mag objectives are essential features for approaching thick biological samples. We will demonstrate the capabilities of this integrated workflow for high-content analysis of small animal models, organoids and tissues, with real case examples on a number of thick samples commonly imaged in any imaging facility.

Structured Illumination Microscopy (SIM) has become a key approach for super-resolution imaging. However, classical SIM and conventional systems are often limited by trade-offs in speed, resolution, photobleaching control, and workflow flexibility, particularly in demanding live and volumetric imaging applications.

This workshop presents CSR Biotech’s next-generation MI-SIM platform, addressing these limitations through advanced hardware–software integration. During the session, we will demonstrate high-speed and high-resolution live-cell imaging and explore the power of real-time reconstruction. We will also highlight how Sparse deconvolution allows for the extended imaging of sensitive samples without sacrificing structural detail.

We will further examine the platform’s multiple imaging modes, including TIRF-SIM and 3D-SIM. Finally, we will showcase smart imaging features such as AI-based cell division detection and cell tracking for time-series analysis. These advancements illustrate a highly automated and intelligent SIM imaging workflow.

This EVIDENT workshop presents the FLUOVIEW FV5000 confocal system on the IX85 platform, with a focus on workflow automation, reproducibility, and quantitative imaging enabled by the FLUOVIEW SMART AI guided software interface and integrated performance monitoring tools.

The session introduces how FLUOVIEW SMART streamlines the imaging process by automating key setup steps, including sample detection, focus alignment, and laser power optimization. By removing manual adjustment layers, the system reduces user-dependent variability and enables consistent imaging outcomes across users, experiments, and sites.

A dedicated part of the workshop covers the Microscope Performance Monitor, demonstrating how system performance can be tracked, validated, and documented over time especially in a muti-user environment In addition to our Laser Power Monitor and and SilVIR detector, this enables reproducible imaging conditions and supports long-term data comparability and truly quantitative imaging experiments

The workshop further highlights how the FV5000 supports reliable, quantitative fluorescence imaging through controlled photon detection and a wide dynamic range. This allows users to move from relative intensity measurements toward more robust and reviewable data, addressing increasing demands for reproducibility and data integrity in advanced imaging workflows.

PlantScope is a turnkey live-cell imaging platform designed for long-term observation of growing plant roots with automated tracking. The system enables researchers to follow root tip dynamics over hours to days while maintaining plants in a physiologically relevant upright growth orientation using a unique vertical stage design.

A key capability of PlantScope is automated live root tip tracking. As roots grow and change direction, the system continuously follows the advancing tip and acquires high-resolution image montages that capture the entire developmental cycle, including the meristematic, elongation, and differentiation zones at every time point. This enables comprehensive visualization of root growth dynamics, cell division patterns, tropic responses, and developmental trajectories across extended time-lapse experiments.

At the core of PlantScope, SlideBook and the Yokogawa CSU-W1 spinning disk confocal enable robust capture of dynamic biological processes in realtime. The CSU-W1 delivers rapid acquisition speeds, high sensitivity and multi-color sub-cellular imaging optimized for live samples. SlideBook software seamlessly coordinates all hardware – including microscope, camera, stage, spinning disk, laser launch, NIR lamp – in combination with automated tracking and adaptive imaging workflows in a familiar, easy-to-use interface.

In this workshop, we will demonstrate the PlantScope Marianas multimodal microscopy system with root tip tracking capabilities, showcasing automated long-term imaging workflows and high-resolution visualization of dynamic root development.

S. Untucht, P. Rodriguez, M. Beljan, K. Ritschel, A. Wede, A. Schmitz, B. Deissler, Z. Jiang, V. Augustin, J. Pearson, S. Schicktanz, F. Fahrbach, J. Reddington

Leica Microsystems CMS GmbH, Mannheim, Germany.

Here we present the new Leica Viventis SCAPE, based on oblique plane microscopy (OPM)¹ and swept confocally aligned planar excitation (SCAPE)²,³ technologies.

This system is designed for high-speed, high-throughput volumetric light sheet imaging and is compatible with commonly used carriers, including glass slides, Petri dishes, and well plates. Viventis SCAPE enables fast 3D imaging without the need for specialized holders or deviations from common sample mounting protocols.

We demonstrate the intuitively designed Viventis SCAPE workflow, from straightforward sample mounting in standard carriers and streamlined software integration to fast volumetric acquisition. Practical examples will illustrate how Viventis SCAPE enables high-speed 3D imaging of a wide range of specimens, including 2D cell culture, 3D tissues, organoids, and small organisms, providing new opportunities for observing biological dynamics in real time.

Join this session to discover how Viventis SCAPE delivers fast, gentle, and accessible light sheet imaging—bringing high-speed 3D microscopy into standard laboratory workflows.

1. Dunsby, C. et al. Optically sectioned imaging by oblique plane microscopy. Opt. Express 16, 20306–20316 (2008).

2. Bouchard, M. B. et al. Swept confocally-aligned planar excitation (SCAPE) microscopy for high-speed volumetric imaging of behaving organisms. Nat. Photonics 9, 113–119 (2015).

3. Voleti, V. et al. Real-time volumetric microscopy of in vivo dynamics and large-scale samples with SCAPE 2.0. Nat. Methods 16, 1054–1062 (2019).

Visualizing the three-dimensional architecture of complex biological specimens has traditionally required labor-intensive workflows and imaging times ranging from hours to days. To overcome these limitations, Miltenyi Biotec offers an integrated 3D imaging ecosystem that streamlines sample processing, acquisition, visualization, and analysis. In this workshop, we will present live demonstrations of the UltraMicroscope Blaze™ and its latest innovations for high-speed, scalable light sheet imaging. Attendees will experience the new LightSpeed mode, enabling up to 60-fold faster image acquisition for large-volume specimens, alongside the MACS iQ View – 3D Large Volume package for efficient visualization and handling of large multidimensional datasets with OCTAGON PRIME. We will further highlight an integrated 3D/2D workflow combining volumetric imaging with high-plex spatial analysis, providing a comprehensive solution for accelerated, data-rich biological discovery.

Steep learning curves, inconsistent image quality, and long scanning times are common challenges in slide scanning workflows. The NIS-Elements Slide Scanning solution overcomes them with an intuitive, guided workflow and pre optimized settings that eliminate the need for extensive training. High quality optics and AI powered focusing ensure sharp, reliable images while automatically identifying and capturing all regions of interest at optimal settings. High speed scanning and precise tiling reduce whole tissue acquisition to under a minute, and a streamlined gallery view enables easy visualization and downstream analysis directly within NIS Elements.

The NIS Elements Slide Scanning module is a powerful software solution in combination with the reliable Ni E upright microscope offers:

• Scanning of up to 8 standard or 4 double slides simultaneously

• Full automation combined with premium grade optics

• High resolution, high speed imaging in both brightfield and fluorescence

• An intuitive, user friendly interface

During the workshop, the complete workflow of the scanner will be demonstrated alongside practical examples showing how acquired images can be further enhanced using the powerful post processing and analysis tools available within the NIS Elements software.

Explore the future of slide scanning by registering for the upcoming workshop and discover how this innovative solution can enhance research workflows and laboratory efficiency.

Reserve a spot today and take the next step in advancing microscopy capabilities with Nikon.

Confocal imaging has long been tied to large, complex systems—requiring darkrooms, extensive training, and ongoing specialist support. These barriers often slow down researcher independence and delay results and publications. On top of that, traditional high-end confocal platforms demand continuous, costly maintenance.

Oxford Instruments BC43 changes the game. In a footprint no larger than a printer, BC43 delivers powerful, high-quality confocal imaging—without the complexity of traditional systems. Built with a unique quality control program, every system is guaranteed to deliver consistent, reliable image quality.

Designed around real user needs, BC43 offers unmatched flexibility: start with widefield imaging and upgrade in the field to confocal or super-resolution as your needs evolve.

The new Fusion Benchtop software takes productivity even further, introducing advanced capabilities such as:

• Irregular and multiple irregular montage

• Focus maps for complex samples.

• High-performance large data acquisition

All within an intuitive, easy-to-use workflow.

In this workshop, you will learn how to:

1. Start imaging immediately — no special training required

2. Capture large 3D datasets quickly and effortlessly

3. Acquire high-quality 2D/3D data with seamless stitching

4. Boost productivity using the new powerful Fusion Benchtop features.

Join us to discover how BC43 delivers high-end confocal performance, outstanding image quality, and true ease of use—all in a compact benchtop system designed to accelerate your research.

A hands-on introduction to DNA-PAINT imaging, from experimental design to structures redefined.

DNA-PAINT is a Single-Molecule Localization Microscopy (SMLM) technique that enables the precise visualization of structures with high-resolution through transient DNA binding events.

This workshop will cover the full ONI DNA-PAINT workflow: from experimental design and sample preparation to image acquisition and quantitative analysis using CODI. The focus is on building a practical understanding of how to design robust experiments, acquire high-quality datasets, and extract quantitative information from nanoscale biological structures.

This hands-on session is ideal for researchers looking to get started with DNA-PAINT and develop confidence in end-to-end super-resolution imaging workflows.

Quantitative time-resolved fluorescence techniques like Fluorescence Lifetime Imaging (FLIM) have become more attractive recently to study mechanisms driven by phase separation or to sense the cellular environment, for example.

PicoQuant`s innovative confocal microscope Luminosa combines state-of-the-art hardware with cutting edge software to deliver high quality data while simplifying daily operation. The software includes several features which improve the ease of use and reproducibility of experiments, including context-based workflows, sample-free auto-alignment and excitation laser power calibration. Still, if required for new method development every optomechanical component can be fully accessible.

We will show how FLIM is streamlined with Luminosa.. In combination with GPU-accelerated algorithms, this enables high-speed automated analysis of FLIM images. The InstaFLIM analysis workflow suggests the best fitting model based on statistical arguments, requiring minimal user interaction. The additional NovaFLIM software (also available for FLIM images acquired with LSM Upgrade kits) enables more extensive and advanced image analysis offering an holistic FLIM analysis process including exponential decay analysis, phasor plots and pattern matching.

The design of Luminosa`s software makes all data easily accessible. It works with the open, well documented PTU data format, enabling custom analysis while in includes enhanced, well structured metadata and ome-tiff exports.

Multiphoton microscopy has become an essential tool for studying neuronal activity, circuit dynamics, and complex biological processes in living specimens. However, advanced imaging experiments have traditionally depended on highly customized systems, specialist alignment knowledge, and careful integration of many optical, mechanical, electronic, and laser components.

This workshop presents Thorlabs’ evolving component-to-system approach to multiphoton microscopy, developed through a large portfolio of photonics, optomechanics, objectives, detectors, motion control, and imaging technologies. We will discuss how Thorlabs’ long-standing component expertise has expanded into customer-inspired turnkey and modular microscopy platforms, and how this system-level experience now feeds back into the development of broadly useful microscopy components for the wider life-science community.

As an example of this approach, we will highlight the Prelude® Functional Imaging Microscope, a compact, fully integrated two-photon platform designed to simplify access to in vivo functional imaging. Developed in collaboration with researchers from Baylor College of Medicine and Columbia University, Prelude combines fiber-delivered femtosecond excitation, alignment-free operation, sensitive silicon photomultiplier detection, and flexible sample access in a maneuverable platform optimized for GFP and GCaMP imaging. In this workshop, excitation is provided by a TOPTICA Photonics FemtoFiber Ultra 920 nm laser, illustrating the role of close technology partnerships in delivering robust imaging solutions.

Beyond the microscope platform itself, the workshop will show how lessons learned from turnkey systems help identify missing capabilities and inspire enabling technologies, including life-science-optimized objectives, silicon photomultiplier detector modules, remote focusing options, modular microscope architectures, and compact imaging tools such as mini2P.

Holotomography (HT) is a powerful tool for label-free, quantitative 3D imaging of living cells. By reconstructing the three-dimensional refractive index (RI) distribution of biological specimens, HT reveals subcellular structures—including nuclei, mitochondria, and lipid droplets—without the need for exogenous labels or staining, preserving the native physiological state of cells throughout the experiment.

In this workshop, we introduce the HT-X1 mini, the latest addition to Tomocube’s HT-X1 series. The HT-X1 mini brings full HT performance to an affordable, everyday-ready platform designed to lower the barrier to high-quality label-free imaging. As a table-top system, it fits seamlessly into culture rooms and space-limited environments, and operates via a laptop-based workflow with minimal setup requirements.

Despite its compact form factor, the HT-X1 mini delivers comparable performance to other HT-X1 series systems, supporting high-resolution 3D structural visualization and quantitative RI-based analysis through TomoAnalysis™ workflows. The system is expandable with optional modules—including a stage-top incubator for long-term live-cell imaging, a fluorescence light engine for target-specific signals, additional HT wavelengths, and a laser-based autofocus sensor—making it adaptable as research needs evolve.

This workshop will present representative applications enabled by the HT-X1 mini, including cell morphology observation, quantitative phenotyping, and routine live-cell culture monitoring. We will demonstrate how RI-based imaging provides immediate biological insight—from single-cell morphodynamics to subcellular organelle tracking—enabling rich structural insight from label-free RI data. Overall, we aim to show that HT is no longer a specialized capability reserved for a few labs, but an accessible, everyday imaging modality available to any cell biology researcher.

Super-resolution microscopy has made possible the exploration of biological systems at the nanoscale. One historical technique, STORM, uses a redox buffer to intermittently switch the fluorophores into a dark state to illuminate only a subset of molecules at a time and allows Single-Molecule Localization Microscopy (SMLM). Each blinking event is fitted to localize the position of the emitter with a 10 – 20 nm spatial resolution, enabling the visualization of cellular structures and molecular organization at the nanoscale. In the past, STORM was generally conducted on a single target because the use of different fluorophores introduced chromatic aberrations and complexity in the design of imaging buffer composition.

In this workshop, we demonstrate Abbelight multiplexed STORM imaging, enabled by the SAFe platform workflow, which features Spectral Demixing, an advanced multi-color detection design. By imaging far-red dyes with small differences in emission spectra, Spectral Demixing allows the simultaneous imaging of up to 3 targets with a single imaging buffer and produces aberration-free colocalization analysis. In Abbelight NEO LiveImagingTM software, users have access to real time demixing of the targets, allowing them to decide early on if a FOV is worth the full acquisition and saving precious time on the instrument. Abbelight NEO AnalysisTM software integrates a bias-free demixing algorithm, giving the option to the user to choose between high data recovery, optimal demixing and minimal crosstalk. The result: a streamlined and accelerated imaging strategy that reduces acquisition time, avoids chromatic abberrations and maintains the highest colocalization accuracy with minimal crosstalk.

Modern, high throughput 3D microscopy not only requires solutions for rapid image acquisition, but also for scalable, on-the-fly image data transfer, image processing and content analysis. This workshop presents an integrated imaging and computing workflow combining the Bruker Luxendo light-sheet microscope with the Acquifer HIVE data management, a multi-user, high performance computing platform.

Multi-camera, multi-view light sheet microscopes enable fast volumetric imaging of complex 3D biological samples such as spheroids, organoids, and developing organisms, delivering high optical efficiency and gentle illumination across multiple imaging dimensions. To match this acquisition speed, imaging data is directly streamed to Acquifer HIVE, where it is centrally stored, processed, and made immediately available for analysis and word-wide collaboration, all in an integrated workflow made available by smart Hardware and Software implementation. The workshop demonstrates how end to end optimization—from image acquisition to compute accelerated processing—removes data bottlenecks and enables efficient, reproducible 3D microscopy workflows for microscopists, imaging facilities and translational research.

For decades, the pursuit of sub-diffraction resolution has forced researchers into uncomfortable trade-offs: resolution gained at the cost of phototoxicity, imaging speed sacrificed for optical sectioning quality, or biological relevance compromised by sample preparation demands. ZEISS Lattice SIM 5 was designed from the ground up to resolve these conflicts.

At the core of the system lies a fundamentally different approach to structured illumination. Rather than projecting conventional grid patterns across a single plane, Lattice SIM generates an optimized three-dimensional lattice of light spots that structures illumination throughout the full depth of the sample. This produces sharper interference patterns, dramatically higher contrast, and half the light dose of classic SIM for the same information content. The SIM² image reconstruction algorithm then transforms the acquired data into strikingly crisp, super-resolved images — in both fixed and living specimens.

In this hands-on workshop session, participants will explore the full versatility of the platform — from resolving subcellular architecture at high resolution to imaging dynamics in living cells and optically sectioning thick 3D models. We will also discuss how to match imaging mode to biological question, and demonstrate how the ZEN software environment integrates acquisition, reconstruction analysis into a single streamlined workflow.

Mechanobiology is an emerging field in life science, and the role of mechanical cues has gained relevance in the understanding of the physical state and function of biological systems. However, current gold standard techniques to measure biomechanical properties are invasive, mainly contact-based and limited to surface probing, they lack 3D sub-cellular resolution capabilities or they are not always suitable for multicellular environments.

Confocal Brillouin microscopy has recently emerged as an all-optical, contact- and label-free technique for in vivo 3D subcellular mapping of elasticity and viscosity distribution within cells and tissues, even in settings where physical contact is not possible, providing sufficient sensitivity to detect and monitor relevant biomechanical changes. In this talk, we will walk you through the technological challenges and advancements of the technique, showing representative examples of real biological experiments that highlight the potential of this technique, especially when integrated in an advanced spinning disk correlative confocal imaging platform

Modern microscopy is no longer defined by a single technique. Each modality brings unique strengths and limitations, and it is the combination of these approaches that unlocks the full potential of biological imaging. To truly maximize information from complex samples, multi-modal integration has become essential especially in super-resolution imaging. However, current workflows are often limited by fragmented software, inefficient modality switching, and challenging cross-modality data integration.

CSR Biotech’s multi-modal super-resolution solutions overcome these challenges, enabling seamless integration of Structured Illumination Microscopy (SIM), Spinning Disk and label-free Optical Diffraction Tomography (ODT) imaging modalities. In this workshop, we will focus on dual-modality acquisitions with hands-on SIM and ODT, unified and automated workflow tools, intelligent image registration, and event-based triggering for smart microscopy to simplify complex experiments in multi-modal imaging.

Finally, we will introduce the FINER multi-modal image processing suite, which includes tools for resolution enhancement such as SIM and SACD, AI-assisted denoising and segmentation.

This EVIDENT workshop introduces the FLUOVIEW FV5000MPE platform on the IX85 inverted microscope, focusing on compact and convenient multiphoton integration for deep tissue imaging applications. We will present to you the easy integration of pig tailed fibre lasers on top of a confocal imaging platform to achieve a multiphoton ready combo system. The session highlights how this design reduces system complexity and footprint while maintaining high-performance imaging capabilities. Attendees will understand how fiber-based laser delivery supports more robust alignment, easier maintenance, and improved day-to-day usability, making multiphoton imaging more accessible in shared environments.

A dedicated part of the workshop covers SilVIR non-descanned detectors (NDD), designed for high-sensitivity photon detection in scattering specimens. Optimized detection geometry combined with photon-counting capabilities improves signal collection efficiency at depth while maintaining stable and quantitative imaging conditions.

Together, these elements position the FV5000MPE as a solution that brings multiphoton imaging closer to routine use, balancing deep imaging performance with operational simplicity and system stability within the EVIDENT platform.

PlantScope is a turnkey live-cell imaging platform designed for long-term observation of growing plant roots with automated tracking. The system enables researchers to follow root tip dynamics over hours to days while maintaining plants in a physiologically relevant upright growth orientation using a unique vertical stage design.

A key capability of PlantScope is automated live root tip tracking. As roots grow and change direction, the system continuously follows the advancing tip and acquires high-resolution image montages that capture the entire developmental cycle, including the meristematic, elongation, and differentiation zones at every time point. This enables comprehensive visualization of root growth dynamics, cell division patterns, tropic responses, and developmental trajectories across extended time-lapse experiments.

At the core of PlantScope, SlideBook and the Yokogawa CSU-W1 spinning disk confocal enable robust capture of dynamic biological processes in realtime. The CSU-W1 delivers rapid acquisition speeds, high sensitivity and multi-color sub-cellular imaging optimized for live samples. SlideBook software seamlessly coordinates all hardware – including microscope, camera, stage, spinning disk, laser launch, NIR lamp – in combination with automated tracking and adaptive imaging workflows in a familiar, easy-to-use interface.

In this workshop, we will demonstrate the PlantScope Marianas multimodal microscopy system with root tip tracking capabilities, showcasing automated long-term imaging workflows and high-resolution visualization of dynamic root development.

D. Gambarotto¹, A. Fulterer¹, D. Migliozzi¹, G. de Medeiros², Q. Juppet¹, M. Benton³, A. Boni¹, P. Liberali², S. Schicktanz¹, J. Reddington¹, P. Strnad¹.

1. Leica Microsystems, Lausanne, Switzerland; 2. Friedrich Miescher Institute for Biomedical Research, Basel, Switzerland; 3. EMBL, Heidelberg, Germany.

Light sheet fluorescence microscopy is a key technology for both long-term live imaging and the visualization of large, optically cleared samples¹. However, most systems are optimized for either live or cleared imaging, making transitions between the two complex and inefficient.

Leica Viventis Deep overcomes this limitation by enabling seamless exchange between live and cleared imaging within a single platform. Its open-top configuration with dual illumination and dual-view detection supports gentle, high-speed live imaging², while an easily exchangeable objective block allows easy adaptation to cleared sample imaging with multi-immersion optics supporting samples with RI ranging from 1.33 up to 1.56.

This flexibility eliminates the traditional trade-off between live and cleared imaging, enabling users to switch workflows quickly without compromising performance or deviating from standard sample preparation.

In this workshop, we will demonstrate the simplicity and efficiency of the Viventis Deep workflow—from sample mounting to acquisition and 3D visualization—across a broad range of applications.

References

1. Huisken, J. et al. Optical sectioning deep inside live embryos by selective plane illumination microscopy. Science 305, 1007–1009 (2004).

2. Moos, F. et al. Open-top multisample dual-view light-sheet microscope for live imaging of large multicellular systems. Nat. Methods 21, 798–803 (2024).

Visualizing the three-dimensional architecture of complex biological specimens has traditionally required labor-intensive workflows and imaging times ranging from hours to days. To overcome these limitations, Miltenyi Biotec offers an integrated 3D imaging ecosystem that streamlines sample processing, acquisition, visualization, and analysis. In this workshop, we will present live demonstrations of the UltraMicroscope Blaze™ and its latest innovations for high-speed, scalable light sheet imaging. Attendees will experience the new LightSpeed mode, enabling up to 60-fold faster image acquisition for large-volume specimens, alongside the MACS iQ View – 3D Large Volume package for efficient visualization and handling of large multidimensional datasets with OCTAGON PRIME. We will further highlight an integrated 3D/2D workflow combining volumetric imaging with high-plex spatial analysis, providing a comprehensive solution for accelerated, data-rich biological discovery.

3D microscopy has advanced rapidly with spheroid and organoid models, which closely replicate native tissue. While acquiring quality 3D images is challenging, developing robust and reproducible analysis workflows can be even more difficult, often requiring Python expertise and limiting accessibility. NIS Elements addresses this with an intuitive, visual programming interface, allowing users to drag and drop functions for 2D/3D processing, measurement, plotting, and data management—eliminating the need for scripting while maintaining flexibility.

For specialized analyses, users can incorporate open‑source algorithms via integrated Python scripting and LLM prompts, enabling easy access to tools like Cellpose3, Cellpose SAM, Stardist, and others. If these pretrained models lack desired performance, NIS Elements supports custom 3D deep learning training for segmentation, signal enhancement, and channel prediction, using user data for higher accuracy and speed. To handle computational demands from large datasets, Cluster Computing software distributes tasks across idle lab computers.

In this workshop, we will show how to:

• Use visual programming for 3D image analysis workflows

• Make use of pretrained open-source segmentation models inside NIS-Elements

• Create 3D binaries with the help of detect.ai and train custom 3D segmentation model

• Use LLM to make a Python script to be used inside NIS-Elements

• Run Batch Analysis using Cluster Computing on Octagon PRIME – high performance processing unit from Octagon Data GmbH

Finally, you can use your 3D analysis pipeline as part of acquisition to enable feedback driven microscopy workflows, removing the need for users to remain physically present at the microscope.

The BC43 Benchtop Spinning Disk Confocal System, developed by Oxford Instruments, is designed to address the critical challenges of high-containment Biosafety BSL-2+/3/4 laboratories. This compact, high-performance system is validated for extreme fumigation protocols, ensuring safe decontamination and uninterrupted operation without voiding warranty. Its lightweight, light-tight benchtop design eliminates the need for dedicated darkrooms or additional infrastructure, making it ideal for restricted laboratory spaces.

Key Innovations

A key innovation of the BC43 is its comprehensive service support package, specifically tailored for BSL-2+/3/4 environments where external service visits are often impractical due to containment constraints. The system includes a dedicated BSL service solution that provides:

• Spare parts to enable rapid on-site repairs, minimizing downtime

• Remote support tools to troubleshoot issues in real-time, reducing the need for physical intervention and ensuring compliance with containment protocols.

• Certified training programs for customers, empowering facility staff to perform routine maintenance and servicing under PPE constraints.

• By integrating performance, containment adaptability, and proactive service support, the BC43 delivers a fully autonomous imaging solution for high-containment research, eliminating service gaps and maintaining workflow integrity.

A key innovation of the BC43 is its support for defined fumigation workflows, enabling routine full system decontamination while maintaining system integrity.

The Fusion software, equipped with guided protocols, facilitates fast and reliable data acquisition even under PPE constraints.

By combining performance, containment adaptability, and ease of use, the BC43 provides a robust solution for advanced bio-imaging in high-containment research facilities.

Harness the power of ONI’s super-resolution expertise to characterise your nanoparticles with ease.

Extracellular vehicles (EVs) play key roles in cell-to-cell communication, crossing biological barriers, and specifically getting internalised, making them ideal candidates for drug delivery methods and disease diagnostics. Similarly, lipid nanoparticles (LNPs) are advanced delivery systems that encapsulate therapeutic agents and mRNA to protect them from degradation and facilitate their entry into target cells.

Critical nanoparticle characterisation metrics to verify both EV and LNP sample heterogeneity and functionality include particle size, biomarker positivity, cargo loading, and ligand engineering (in the case of LNPs).

This workshop showcases how ONI’s SMLM-based workflows can uniquely address current gaps in nanoparticle characterisation, from the population level down to the single-particle level using sample preparation kits, automated imaging, and data analysis tools.

This session is intended for researchers working with EVs, exosomes, lipid nanoparticles, or related fields. During this session, we will demonstrate that the ONI Nanoimager can acquire, image, and analyse datasets in a fully automated way!

Recently, high-performance SPAD-arrays featuring few tens of pixels have become available. Combining these with suitable multi-channel TCSPC-devices enables time-resolved Image Scanning Microscopy (ISM). ISM enhances the spatial resolution and increases image contrast compared to standard confocal imaging. FLIM can provide additional functional information as well as extended marker multiplexing using lifetime contrast. So both technologies complement each other.

In this workshop we will show how the PDA23 SPAD Array is implemented in Luminosa. The newly released software NovaISM enables the analysis of ISM-FLIM images acquired with the PDA-23 Add-On of the Luminosa microscope. Image scanning microscopy (ISM) with a SPAD array detector achieves resolution enhancements of about 1.5 to 1.7 times in comparison to standard confocal images, in combination with deconvolution. Even for 2d-recordings/data the contrast of the ISM-FLIM images is enhanced significantly by rejecting the out-of focus light. Such rejection enhances not only the signal-to-noise-ratio, but also the lifetime contrast of the FLIM images. These benefits enable either faster image acquisition or gentler imaging of live samples.

Multiphoton microscopy has become an essential tool for studying neuronal activity, circuit dynamics, and complex biological processes in living specimens. However, advanced imaging experiments have traditionally depended on highly customized systems, specialist alignment knowledge, and careful integration of many optical, mechanical, electronic, and laser components.

This workshop presents Thorlabs’ evolving component-to-system approach to multiphoton microscopy, developed through a large portfolio of photonics, optomechanics, objectives, detectors, motion control, and imaging technologies. We will discuss how Thorlabs’ long-standing component expertise has expanded into customer-inspired turnkey and modular microscopy platforms, and how this system-level experience now feeds back into the development of broadly useful microscopy components for the wider life-science community.

As an example of this approach, we will highlight the Prelude® Functional Imaging Microscope, a compact, fully integrated two-photon platform designed to simplify access to in vivo functional imaging. Developed in collaboration with researchers from Baylor College of Medicine and Columbia University, Prelude combines fiber-delivered femtosecond excitation, alignment-free operation, sensitive silicon photomultiplier detection, and flexible sample access in a maneuverable platform optimized for GFP and GCaMP imaging. In this workshop, excitation is provided by a TOPTICA Photonics FemtoFiber Ultra 920 nm laser, illustrating the role of close technology partnerships in delivering robust imaging solutions.

Beyond the microscope platform itself, the workshop will show how lessons learned from turnkey systems help identify missing capabilities and inspire enabling technologies, including life-science-optimized objectives, silicon photomultiplier detector modules, remote focusing options, modular microscope architectures, and compact imaging tools such as mini2P.

Holotomography (HT) is a powerful tool for label-free, quantitative 3D imaging of living cells. By reconstructing the three-dimensional refractive index (RI) distribution of biological specimens, HT reveals subcellular structures—including nuclei, mitochondria, and lipid droplets—without the need for exogenous labels or staining, preserving the native physiological state of cells throughout the experiment.

In this workshop, we introduce the HT-X1 mini, the latest addition to Tomocube’s HT-X1 series. The HT-X1 mini brings full HT performance to an affordable, everyday-ready platform designed to lower the barrier to high-quality label-free imaging. As a table-top system, it fits seamlessly into culture rooms and space-limited environments, and operates via a laptop-based workflow with minimal setup requirements.

Despite its compact form factor, the HT-X1 mini delivers comparable performance to other HT-X1 series systems, supporting high-resolution 3D structural visualization and quantitative RI-based analysis through TomoAnalysis™ workflows. The system is expandable with optional modules—including a stage-top incubator for long-term live-cell imaging, a fluorescence light engine for target-specific signals, additional HT wavelengths, and a laser-based autofocus sensor—making it adaptable as research needs evolve.

This workshop will present representative applications enabled by the HT-X1 mini, including cell morphology observation, quantitative phenotyping, and routine live-cell culture monitoring. We will demonstrate how RI-based imaging provides immediate biological insight—from single-cell morphodynamics to subcellular organelle tracking—enabling rich structural insight from label-free RI data. Overall, we aim to show that HT is no longer a specialized capability reserved for a few labs, but an accessible, everyday imaging modality available to any cell biology researcher.

Super-resolution microscopy has transformed the way we explore biological systems. One popular technique, DNA-PAINT, exploits the intermittent binding of fluorescent DNA to their targets to illuminate only a subset of molecules at a time and allows Single-Molecule Localization Microscopy (SMLM). Each blinking event is fitted to localize the position of the emitter with a 10 – 20 nm spatial resolution, enabling the visualization of cellular structures and molecular organization at the nanoscale. In the past, DNA-PAINT generally suffered from small fields of view (FOV) and lengthy acquisitions, limiting the overall throughput of data collection.

In this workshop, we demonstrate Abbelight high-throughput DNA-PAINT imaging, enabled by the SAFe platform workflow, which features ASTER illumination, a proprietary flat field excitation technology. Our system enables an ultra-wide 230 x 230 µm fields-of-view and the illumination power is 95% homogeneous across the whole FOV ensuring consistent fluorophore excitation from center to edge and reliable quantifications. In Abbelight NEO LiveImagingTM software, users have access to real-time processing of the data, allowing them to decide early on if a FOV is worth the full acquisition and saving precious time on the instrument. By programming automated acquisitions, the Multi-Dimensional Acquisition (MDA) enables stitching of millimeter-sized regions, plate screening and target multiplexing using the Smartflow microfluidic system to conduct exchange-PAINT. In Abbelight NEO AnalysisTM software, the large datasets can be batched-processed overnight and favor time optimization. The result: higher statistical power and data-rich acquisitions that open the door to population level insights and rare event detection.

Modern fluorescence microscopy applications need to be performed in natural environments of the structures of interests. This requires using large samples, like tissue slices, organoids/spheroids or even whole model organisms. One of the main limitations of Single Molecule Localization Microscopy microscopes is their weak ability to image structures deeper than few nm of the coverslip.

Engineered with simplicity and functionality in mind, the Bruker Vutara VXL system is different, as it uses robust widefield illumination in combination with the patented Biplane detection for 3D imaging. With this combination entire volumes in up to 50 µm depth inside the sample can be resolved in 3D with 20 nm resolution. The comprehensive processing and analysis pipeline in the SRX software package provides insights into the 3D-distribution of the detected molecules within cells in their native environment.

ZEN software from ZEISS provides multiple tools to approach Smart Microscopy. These tools will be demonstrated in two separate workshops. The first workshop is designed for users without scripting experience and will focus on GUI-based procedures for typical event- or phenotype-driven high-resolution imaging using the Guided Acquisition. The second workshop targets advanced coders and will highlight custom Smart Imaging techniques utilizing the ZEN-API.

Advanced imaging techniques have become crucial tools in biomedical research, enabling scientists to explore and document intricate biological structures and their dynamics across scales, from single biomolecules to entire animals.

Image acquisition workflows include positioning of the sample inside the microscope, finding the imaging target and setting the acquisition parameters. Traditionally, these routine steps are often performed manually. While manual operation gives experimenters control over the acquisition process, this limits applicability and scalability.

Smart Imaging approaches circumvent these limitations, by combining imaging with on-the-fly processing of acquired data to trigger changes in the imaging settings. Using live data to steer the acquisition or sample perturbation broadens the capabilities of microscopes to new types of experiments. For example, high-resolution imaging of a specific (rare) phenotypes becomes accessible when identifying the object of interest in a low-resolution overview beforehand, followed by switching to a high-resolution imaging modality only for the relevant field-of-view determined by image analysis.

This workshop presents the EVIDENT IXplore IX85 SpinXL system, combining high-speed spinning disk confocal imaging with an expanded field of view enabled by FN26.5 optics. Built on the IX85 platform, and powered by CrestOptics spinning disk technology the system is designed to capture more biologically relevant context per frame, reducing the need for stitching while increasing experimental throughput.

The workshop focuses on how large field-of-view imaging directly impacts workflow efficiency in live-cell and high-content applications. By acquiring larger areas in a single shot, users can monitor more cells simultaneously, improve statistical relevance, and reduce acquisition time. The spinning disk architecture further supports gentle imaging conditions, minimizing phototoxicity and enabling longer time-lapse experiments.

Participants will explore how the system balances speed, sensitivity, and scalability, making it suitable for shared environments such as imaging core facilities. Emphasis is placed on consistent data generation across users, simplified operation, and adaptability to different experimental needs.

The quality of your biological insights is often determined by the sophistication of your post-processing. This workshop focuses on FINER, our comprehensive software package designed to extract the maximum information from raw image data. We will explore advanced computational techniques such as Sparse and SACD deconvolution, alongside AI denoising to enhance signal-to-noise ratios. Attendees will learn practical strategies for managing common imaging artifacts, including drift correction, bleach correction, and efficient batch processing for large datasets. Whether working with short SIM acquisitions or fixed-cell samples, this session demonstrates how to refine your data into high-fidelity, quantitative results while maintaining the highest standards of image integrity.

Understanding how molecular expression patterns relate to tissue structure and cellular context remains a central challenge in spatial biology. In this context, multiplexed imaging is particularly powerful, as it allows multiple biomarkers to be analysed simultaneously, providing deeper insight into complex biological processes within a single sample.

The latest development of the SLIDEVIEW VS200 slide scanner is specifically designed to support spatial biology workflows by enabling the acquisition of up to 10 fluorescence channels in a single imaging round. By reducing repeated staining cycles and slide handling, this approach minimizes tissue stress, improves data consistency, and helps preserve sample integrity. In addition, the use of dedicated filter sets eliminates the need for spectral unmixing, resulting in more straightforward downstream analysis and significantly reduced processing time.

Beyond advanced fluorescence capabilities, SLIDEVIEW VS200 combines large area, high throughput slide scanning with true multimodal imaging, including brightfield, polarized light, transmitted light, Structured illumination (VS-SILA) and fluorescence acquisition. This multimodality allows users to generate comprehensive datasets that seamlessly integrate morphological and molecular information from the same tissue section.

PlantScope is a turnkey live-cell imaging platform designed for long-term observation of growing plant roots with automated tracking. The system enables researchers to follow root tip dynamics over hours to days while maintaining plants in a physiologically relevant upright growth orientation using a unique vertical stage design.

A key capability of PlantScope is automated live root tip tracking. As roots grow and change direction, the system continuously follows the advancing tip and acquires high-resolution image montages that capture the entire developmental cycle, including the meristematic, elongation, and differentiation zones at every time point. This enables comprehensive visualization of root growth dynamics, cell division patterns, tropic responses, and developmental trajectories across extended time-lapse experiments.

At the core of PlantScope, SlideBook and the Yokogawa CSU-W1 spinning disk confocal enable robust capture of dynamic biological processes in realtime. The CSU-W1 delivers rapid acquisition speeds, high sensitivity and multi-color sub-cellular imaging optimized for live samples. SlideBook software seamlessly coordinates all hardware – including microscope, camera, stage, spinning disk, laser launch, NIR lamp – in combination with automated tracking and adaptive imaging workflows in a familiar, easy-to-use interface.

In this workshop, we will demonstrate the PlantScope Marianas multimodal microscopy system with root tip tracking capabilities, showcasing automated long-term imaging workflows and high-resolution visualization of dynamic root development.

L.A.J. Alvarez, I. Steinmetz, U. Schwarz, V. Augustin, J. Pearson, H. Birk, F. Hecht, J. Roberti

Leica Microsystems CMS, Am Friedensplatz 3, 68165, Mannheim, Germany.

In this workshop, we present three advances for the Leica Microsystems STELLARIS platform: new detectors, automated correction collar optimization, and real-time AI denoising.

We introduce the new generation of multi-pixel photon counting (MCCP)-based Power HyD detectors, the new Power HyD S and Power HyD N. The Power HyD N is a far-red-shifted detector with enhanced photon detection efficiency in the near-infrared. Together with the updated Power HyD S, both feature improved electronics and cooling to −39 °C, reducing dark counts and enhancing single-photon discrimination. Combined with PowerCounting (1) and White Light Laser excitation up to 790 nm, they enable quantitative multiplexed imaging across visible and NIR spectra while retaining lifetime imaging capabilities.

SmartCORR automatically optimizes motorized correction collar settings to match sample-specific conditions, significantly improving image quality by reducing sample-dependent spherical aberrations caused by variations in coverslip thickness or specimen properties. This eliminates time-consuming manual adjustment and minimizes human bias and error. SmartCorr allows to dynamically make changes for larger samples with inhomogeneous properties in confocal, multiphoton and TauSTED experiments.

To boost image quality on-the-fly AI denoising framework, based on Noise2Noise (2) and trained on detector-specific data, enables real-time denoising during live scanning across all STELLARIS systems, featuring a more linearized response, temporal stabilization, and a hallucination blocker to prevent artefacts in photon-sparse regions with the original raw always preserved.

1. Schweikhard, V. et al. (2020). DOI: 10.1038/d42473-020-00398-0.

2. Lehtinen, J. et al. (2018).

Visualizing the three-dimensional architecture of complex biological specimens has traditionally required labor-intensive workflows and imaging times ranging from hours to days. To overcome these limitations, Miltenyi Biotec offers an integrated 3D imaging ecosystem that streamlines sample processing, acquisition, visualization, and analysis. In this workshop, we will present live demonstrations of the UltraMicroscope Blaze™ and its latest innovations for high-speed, scalable light sheet imaging. Attendees will experience the new LightSpeed mode, enabling up to 60-fold faster image acquisition for large-volume specimens, alongside the MACS iQ View – 3D Large Volume package for efficient visualization and handling of large multidimensional datasets with OCTAGON PRIME. We will further highlight an integrated 3D/2D workflow combining volumetric imaging with high-plex spatial analysis, providing a comprehensive solution for accelerated, data-rich biological discovery.

High-content screening and high-resolution confocal imaging have traditionally relied on separate, specialized setups. Imaging well plates at high resolution with reliable statistical output often involves complex hardware and software integration. The combination of Nikon’s ECLIPSE Ji and AX confocal microscope overcomes these challenges, enabling automatic acquisition of large sample sets at the highest resolution.

ECLIPSE Ji – Ease of Use and Robustness: Ji is an AI-powered acquisition and analysis platform that simplifies microscopy workflows and reduces experiment time. It automates setup steps to ensure reproducible, high-quality imaging with minimal intervention—ideal for users of all experience levels. Key capabilities include:

• Automated well plate detection, sample alignment, brightfield AI autofocus, and AutoSignal.ai for optimized exposure settings.

• Smart Experiments with pre-defined assay protocols for fully automated high-content screening.

• Custom Experiments for designing personalized imaging and analysis workflows with the same automation and reproducibility.

Nikon AX, NSPARC – Superior 3D Imaging: AX system with NSPARC detector delivers exceptional optical performance for diverse magnifications and sample types. Key features include:

• 25 mm field of view with Galvo (8K) and Resonant (2K) scanning for detailed tissue imaging and fast, gentle live-cell acquisition.

• Excellent optical sectioning for deep imaging of complex 3D samples such as organoids.

• High resolving power with simultaneous multicolor imaging of fine 3D structures.

During the workshop, participants will experience an end-to-end 3D spheroid imaging workflow—from automated detection and adaptive Z‑stacking to high‑resolution confocal imaging and downstream analysis—powered by NIS‑Elements JOBS and GA3 modules for smart feedback microscopy.

Modern microscopy produces increasingly large and complex image datasets, yet image analysis often remains repetitive, time-consuming, and difficult to reproduce. Researchers frequently apply identical analysis steps across multiple images while manually documenting parameters and processing decisions, creating challenges for efficiency, reproducibility, and collaboration.

This workshop introduces the Image Analysis Workflows available in Imaris 11 and demonstrates how they can automate repetitive tasks, standardize analysis pipelines, and capture the full analytical process in a transparent, shareable format. Participants will learn how workflows record preprocessing, object creation, filtering, masking, quantification, visualization, and other analysis steps while generating reusable workflow files that can be exported and shared with collaborators.

The session will also highlight the new workflow resources introduced in Imaris 11.1, including application-specific workflows developed by the Imaris team to simplify common analysis tasks and accelerate user training. Attendees will explore practical examples designed to reduce the learning curve and improve analytical consistency.

In addition, the workshop will cover AI-driven segmentation and object classification tools integrated into Imaris workflows. Participants will learn how machine learning–based segmentation can be combined with preprocessing methods such as deconvolution, as well as advanced analysis modules including cell detection, object tracking, and filament or blood vessel tracing.

Finally, attendees will discover how validated workflows can be applied to many similar datasets using batch processing, enabling efficient, reproducible analysis while still allowing expert-driven manual input when required.

Using ONI’s Nanoimager with the Discovery KitTM: dSTORM in cells, the ultimate kit to prepare your samples for super-resolution imaging.

The Nanoimager is a compact and state-of-the-art microscope, offering quantitative analysis for localisation-based imaging (dSTORM, PALM, and DNA-PAINT), single-particle tracking, and single-molecule FRET. Designed to operate on any lab bench and with a footprint smaller than a piece of A4 paper, it simplifies super-resolution imaging.

The ONI Discovery Kit™: dSTORM in cells 2 provides a modular workflow for immunofluorescent labelling in cultured cells, which allows you to confidently detect extra and intracellular proteins in two channels with 20 nm resolution and high sensitivity in your own samples. You provide the cells and custom antibodies; we provide the rest!

This workshop is intended for scientists who are looking to brush up on their knowledge of dSTORM and to apply super-resolution to further their research at the molecular level.

“Smart” or Adaptive Feedback Microscopy or Event Driven Microscopy aims to combine bioimage analysis (and other algorithms) with fully motorized and computer-controlled microscopes to create automated and adaptive measurement sequences. This enables the user for example to monitor transient and rare events in the desired level of detail across a large cell population or over long observation times or to streamline the optimization of acquisition parameters and the batch analysis of multiple measurements in a single script.

Here, we present the development of a scripting concept based on the Python programming language for the fully motorized single-photon counting confocal microscope Luminosa. We illustrate these benefits by presenting specific use cases for automated optimization of acquisition parameters. The framework fully opens up the microscope interface to allow the user to call external python libraries without compromising the workflows and functionalities present in the microscope software, such as the user settings, laser safety interlock, and predefined functionality blocks for auto-alignment, live views, etc. Thus scripting demands for the user remain rather low enabling non-experts to develop their own measurement/analysis pipelines.

We envision that the community will build upon this python interface bringing the rapidly evolving open source activities closer to the commercial instrumentation allowing for more efficient method developments nd granting end users direct access to cutting-edge developments. As an example of all possibilities opened via the python interface a connection of between Luminosa and AI-Agents will be shown.

Multiphoton microscopy has become an essential tool for studying neuronal activity, circuit dynamics, and complex biological processes in living specimens. However, advanced imaging experiments have traditionally depended on highly customized systems, specialist alignment knowledge, and careful integration of many optical, mechanical, electronic, and laser components.

This workshop presents Thorlabs’ evolving component-to-system approach to multiphoton microscopy, developed through a large portfolio of photonics, optomechanics, objectives, detectors, motion control, and imaging technologies. We will discuss how Thorlabs’ long-standing component expertise has expanded into customer-inspired turnkey and modular microscopy platforms, and how this system-level experience now feeds back into the development of broadly useful microscopy components for the wider life-science community.

As an example of this approach, we will highlight the Prelude® Functional Imaging Microscope, a compact, fully integrated two-photon platform designed to simplify access to in vivo functional imaging. Developed in collaboration with researchers from Baylor College of Medicine and Columbia University, Prelude combines fiber-delivered femtosecond excitation, alignment-free operation, sensitive silicon photomultiplier detection, and flexible sample access in a maneuverable platform optimized for GFP and GCaMP imaging. In this workshop, excitation is provided by a TOPTICA Photonics FemtoFiber Ultra 920 nm laser, illustrating the role of close technology partnerships in delivering robust imaging solutions.

Beyond the microscope platform itself, the workshop will show how lessons learned from turnkey systems help identify missing capabilities and inspire enabling technologies, including life-science-optimized objectives, silicon photomultiplier detector modules, remote focusing options, modular microscope architectures, and compact imaging tools such as mini2P.

Holotomography (HT) is a powerful tool for label-free, quantitative 3D imaging of living cells. By reconstructing the three-dimensional refractive index (RI) distribution of biological specimens, HT reveals subcellular structures—including nuclei, mitochondria, and lipid droplets—without the need for exogenous labels or staining, preserving the native physiological state of cells throughout the experiment.

In this workshop, we introduce the HT-X1 mini, the latest addition to Tomocube’s HT-X1 series. The HT-X1 mini brings full HT performance to an affordable, everyday-ready platform designed to lower the barrier to high-quality label-free imaging. As a table-top system, it fits seamlessly into culture rooms and space-limited environments, and operates via a laptop-based workflow with minimal setup requirements.

Despite its compact form factor, the HT-X1 mini delivers comparable performance to other HT-X1 series systems, supporting high-resolution 3D structural visualization and quantitative RI-based analysis through TomoAnalysis™ workflows. The system is expandable with optional modules—including a stage-top incubator for long-term live-cell imaging, a fluorescence light engine for target-specific signals, additional HT wavelengths, and a laser-based autofocus sensor—making it adaptable as research needs evolve.

This workshop will present representative applications enabled by the HT-X1 mini, including cell morphology observation, quantitative phenotyping, and routine live-cell culture monitoring. We will demonstrate how RI-based imaging provides immediate biological insight—from single-cell morphodynamics to subcellular organelle tracking—enabling rich structural insight from label-free RI data. Overall, we aim to show that HT is no longer a specialized capability reserved for a few labs, but an accessible, everyday imaging modality available to any cell biology researcher.