{"id":372,"date":"2024-05-03T09:34:51","date_gmt":"2024-05-03T09:34:51","guid":{"rendered":"https:\/\/ppbi.pt\/wordpress\/?page_id=372"},"modified":"2024-10-18T13:42:16","modified_gmt":"2024-10-18T13:42:16","slug":"icvs","status":"publish","type":"page","link":"https:\/\/ppbi.pt\/wordpress\/index.php\/icvs\/","title":{"rendered":"ICVS-UM"},"content":{"rendered":"\n
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\r\n \"Microscopy\r\n <\/div>\r\n

\r\n Microscopy and Image Facility \u2013 ICVS<\/span>\r\n <\/h1>\r\n<\/div>\n\n\n\n
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Microscopy and Imaging Facility (MIF) of ICVS presents a well-suited platform of equipment to meet the daily challenges of an innovative research institute, able to image very different types of samples: from cells and tissues to biomaterials and microorganisms. To fulfil these requirements, MIF grants access to imaging techniques including Widefield Microscopy (Brightfield, DIC, Phase Contrast, Polarized Light Imaging, Live Imaging and Fluorescence), Laser Scanning Confocal Microscopy (co-localization, Live Imaging, Multi-dimension imaging, FRET and FRAP), Stereology microscopy (Golgi Staining), and also to more differentiated technology as Holotomography, 2-Photon Microscopy and Micromanipulation Microscopy.<\/p>\n

Our strongest fields of expertise are the confocal microscopy, namely the multi-dimension imaging, co-localization studies, FRET and FRAP analysis; and the stereology microscopy, mostly for the analysis of nervous system regarding neuron morphology, cell density, volumetry, neuronal branching, spine count and classification.<\/p>\n

Looking forward to innovate and disseminate knowledge, we offer scientific advice and tailor-made solutions in study design, development and data analysis, while also providing regular hands-on training sessions for all microscopes.<\/p>\n\r\n<\/div>\n\n\n

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Team<\/h2>\r\n \r\n
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\r\n \"Andreia\r\n <\/div>\r\n
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Andreia Teixeira Castro<\/h2>\r\n

Head<\/p>\r\n

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\r\n \"Luc\u00edlia\r\n <\/div>\r\n
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Luc\u00edlia Goreti Pinto<\/h2>\r\n

Staff<\/p>\r\n

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Resources<\/p>\n\t\t\t

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Equipment:<\/strong><\/p>\r\n

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  • Olympus FV3000<\/li>\r\n \r\n
  • Olympus FV1000<\/li>\r\n \r\n
  • Olympus BX61<\/li>\r\n \r\n
  • Olympus IX81<\/li>\r\n \r\n
  • Olympus IX53<\/li>\r\n \r\n
  • Nanolive’s 3D Cell Explorer (Holotomography)<\/li>\r\n \r\n
  • 2Photon Olympus FVMPE-RS<\/li>\r\n \r\n <\/ul>\r\n <\/div>\r\n <\/div>\r\n\r\n
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    Software:<\/strong><\/p>\r\n

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    • Cellsense<\/li>\r\n \r\n
    • Imaris<\/li>\r\n \r\n
    • Neurolucida<\/li>\r\n \r\n
    • Stereo Investigator<\/li>\r\n \r\n
    • Steve<\/li>\r\n \r\n <\/ul>\r\n <\/div>\r\n <\/div>\r\n<\/div>\n\n<\/div>\n\t\t<\/div>\n\n
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      Offered Technologies<\/p>\n\t\t\t

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      • Deconvolution Widefield Microscopy (DWM)<\/li>\r\n \r\n
      • Laser Scanning Confocal Microscopy (LSCM\/CLSM)<\/li>\r\n \r\n
      • F\u00f6rster Resonance Energy Transfer (FRET)<\/li>\r\n \r\n
      • Fluorescence Recovery After Photobleaching (FRAP)<\/li>\r\n \r\n
      • Multiphoton Microscopy<\/li>\r\n \r\n<\/ul>\n\n<\/div>\n\t\t<\/div>\n\n
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        Publications<\/p>\n\t\t\t

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        • Deseyve C, Domingues AV, Carvalho TTA, Armada G, Correia R, Vieitas-Gaspar N, Wezik M, Pinto L, Sousa N, Coimbra B, Rodrigues AJ, Soares-Cunha C. Nucleus accumbens neurons dynamically respond to appetitive and aversive associative learning. J Neurochem. 2024 Mar;168(3):312-327. doi: 10.1111\/jnc.16063. Epub 2024 Feb 5. PMID: 38317429.<\/li>\n<\/ul>\n

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          • Assun\u00e7\u00e3o-Silva RC, Pinho A, Cibr\u00e3o JR, Pereira IM, Monteiro S, Silva NA, Campos J, Rebelo AL, Schlosser G, Pinto L, Pandit A, Salgado AJ. Adipose tissue derived stem cell secretome induces motor and histological gains after complete spinal cord injury in Xenopus laevis<\/em> and mice. J Tissue Eng. 2024 Feb 9;15:20417314231203824. doi: 10.1177\/20417314231203824. PMID: 38343771; PMCID: PMC10858666.<\/li>\n<\/ul>\n

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            • Lentilhas-Gra\u00e7a J, Santos DJ, Afonso J, Monteiro A, Pinho AG, Mendes VM, Dias MS, Gomes ED, Lima R, Fernandes LS, Fernandes-Amorim F, Pereira IM, de Sousa N, Cibr\u00e3o JR, Fernandes AM, Serra SC, Rocha LA, Campos J, Pinho TS, Monteiro S, Manadas B, Salgado AJ, Almeida RD, Silva NA. The secretome of macrophages has a differential impact on spinal cord injury recovery according to the polarization protocol. Front Immunol. 2024 Feb 20;15:1354479. doi: 10.3389\/fimmu.2024.1354479. PMID: 38444856; PMCID: PMC10912310.<\/li>\n<\/ul>\n

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              • Rodrigues RS, Moreira JB, Mateus JM, Barateiro A, Paulo SL, Vaz SH, Louren\u00e7o DM, Ribeiro FF, Soares R, Loureiro-Campos E, Bielefeld P, Sebasti\u00e3o AM, Fernandes A, Pinto L, Fitzsimons CP, Xapelli S. Cannabinoid type 2 receptor inhibition enhances the antidepressant and proneurogenic effects of physical exercise after chronic stress. Transl Psychiatry. 2024 Mar 30;14(1):170. doi: 10.1038\/s41398-024-02877-0. PMID: 38555299; PMCID: PMC10981758.<\/li>\n<\/ul>\n<\/div>\n
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                • Martins-Macedo J, Ara\u00fajo B, Anjo SI, Silveira-Rosa T, Patr\u00edcio P, Alves ND, Silva JM, Teixeira FG, Manadas B, Rodrigues AJ, Lepore AC, Salgado AJ, Gomes ED, Pinto L. Glial-restricted precursors stimulate endogenous cytogenesis and effectively recover emotional deficits in a model of cytogenesis ablation. Mol Psychiatry. 2024 Mar 7. doi: 10.1038\/s41380-024-02490-z. Epub ahead of print. PMID: 38454085.<\/li>\n<\/ul>\n

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                  • Pinho TS, Cibr\u00e3o JR, Silva D, Barata-Antunes S, Campos J, Afonso JL, Sampaio-Marques B, Ribeiro C, Macedo AS, Martins P, Cunha CB, Lanceros-Mendez S, Salgado AJ. In vitro neuronal and glial response to magnetically stimulated piezoelectric poly(hydroxybutyrate-co-hydroxyvalerate) (PHBV)\/cobalt ferrite (CFO) microspheres. Biomater Adv. 2024 May;159:213798. doi: 10.1016\/j.bioadv.2024.213798. Epub 2024 Feb 10. PMID: 38364446.<\/li>\n<\/ul>\n

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                    • Yu Q, Du F, Belli I, Gomes PA, Sotiropoulos I, Waites CL. Glucocorticoid stress hormones stimulate vesicle-free Tau secretion and spreading in the brain. Cell Death Dis. 2024 Jan 18;15(1):73. doi: 10.1038\/s41419-024-06458-3. PMID: 38238309; PMCID: PMC10796385.<\/li>\n<\/ul>\n<\/div>\n<\/div>\n<\/div>\n\n\n<\/div>\n\t\t<\/div>\n\n
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                      Job Shadowing<\/p>\n\t\t\t

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                      • <\/li><\/ul>\n\n<\/div>\n\t\t<\/div>\n<\/div><\/div>\n","protected":false},"excerpt":{"rendered":"","protected":false},"author":5,"featured_media":0,"parent":0,"menu_order":0,"comment_status":"closed","ping_status":"closed","template":"","meta":{"footnotes":""},"class_list":["post-372","page","type-page","status-publish","hentry"],"featured_image_src":null,"yoast_head":"\nICVS-UM - ELMI Booth<\/title>\n<meta name=\"robots\" content=\"index, follow, max-snippet:-1, max-image-preview:large, max-video-preview:-1\" \/>\n<link rel=\"canonical\" href=\"https:\/\/ppbi.pt\/wordpress\/index.php\/icvs\/\" \/>\n<meta property=\"og:locale\" content=\"en_US\" \/>\n<meta property=\"og:type\" content=\"article\" \/>\n<meta property=\"og:title\" content=\"ICVS-UM - ELMI Booth\" \/>\n<meta property=\"og:url\" content=\"https:\/\/ppbi.pt\/wordpress\/index.php\/icvs\/\" \/>\n<meta property=\"og:site_name\" content=\"ELMI Booth\" \/>\n<meta property=\"article:publisher\" content=\"https:\/\/www.facebook.com\/profile.php?id=100063509859246\" \/>\n<meta property=\"article:modified_time\" content=\"2024-10-18T13:42:16+00:00\" \/>\n<meta property=\"og:image\" content=\"https:\/\/ppbi.pt\/wordpress\/wp-content\/uploads\/2024\/05\/banner_Microscopy.jpg\" \/>\n<meta name=\"twitter:card\" content=\"summary_large_image\" \/>\n<meta name=\"twitter:site\" content=\"@PPBioImaging\" \/>\n<meta name=\"twitter:label1\" content=\"Est. reading time\" \/>\n\t<meta name=\"twitter:data1\" content=\"1 minute\" \/>\n<script type=\"application\/ld+json\" class=\"yoast-schema-graph\">{\"@context\":\"https:\\\/\\\/schema.org\",\"@graph\":[{\"@type\":\"WebPage\",\"@id\":\"https:\\\/\\\/ppbi.pt\\\/wordpress\\\/index.php\\\/icvs\\\/\",\"url\":\"https:\\\/\\\/ppbi.pt\\\/wordpress\\\/index.php\\\/icvs\\\/\",\"name\":\"ICVS-UM - 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