{"id":358,"date":"2024-05-03T09:31:49","date_gmt":"2024-05-03T09:31:49","guid":{"rendered":"https:\/\/ppbi.pt\/wordpress\/?page_id=358"},"modified":"2024-10-18T13:44:48","modified_gmt":"2024-10-18T13:44:48","slug":"cnc","status":"publish","type":"page","link":"https:\/\/ppbi.pt\/wordpress\/index.php\/cnc\/","title":{"rendered":"CNC-UC"},"content":{"rendered":"\n
<\/span>\"\"
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<\/p>\n<\/div><\/div>\n\n\n\n

\r\n
\r\n \"Microscopy\r\n <\/div>\r\n

\r\n Microscopy Imaging Center of coimbra (MICC) \u2013 CNC-UC<\/span>\r\n <\/h1>\r\n<\/div>\n\n\n\n
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The Microscopy Imaging Center of Coimbra (MICC), part of the Center for Neuroscience and Cell Biology (CNC), is a centralized facility that provides essential support for both conventional and advanced light microscopy techniques. Equipped with cutting-edge technology, the MICC is accessible to all researchers, whether from academia or industry. Our mission is to enhance the research and educational experience for the broader scientific community.<\/p>\n

To achieve this, the MICC-CNC:<\/p>\n

\u2013 Delivers technical training for local users and visiting researchers;
\n\u2013 Provides expert consultation on experimental design and image analysis;
\n\u2013 Incorporates the latest advancements in hardware and software tailored for biomedical research;
\n\u2013 Continuously offers education on advanced microscopy through training courses and workshops.<\/p>\n\r\n<\/div>\n\n\n

\r\n \r\n Visit Node Website\r\n <\/a>\r\n<\/div>\n<\/div>\n\n\n\n
<\/div>\n<\/div>\n\n\n\n
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Team<\/h2>\r\n \r\n
\r\n \r\n
\r\n
\r\n \"Luisa\r\n <\/div>\r\n
\r\n

Luisa Maria Cortes<\/h2>\r\n

Head<\/p>\r\n

\r\n \r\n <\/i>\r\n <\/a>\r\n <\/p>\r\n <\/div>\r\n <\/div>\r\n \r\n <\/div>\r\n \r\n\r\n \r\n

\r\n
\r\n \r\n
\r\n
\r\n \"Margarida\r\n <\/div>\r\n
\r\n

Margarida Caldeira<\/h2>\r\n

Staff<\/p>\r\n

\r\n \r\n <\/i>\r\n <\/a>\r\n <\/p>\r\n <\/div>\r\n <\/div>\r\n \r\n

\r\n
\r\n \"Tatiana\r\n <\/div>\r\n
\r\n

Tatiana Catarino<\/h2>\r\n

Staff<\/p>\r\n

\r\n \r\n <\/i>\r\n <\/a>\r\n <\/p>\r\n <\/div>\r\n <\/div>\r\n \r\n <\/div>\r\n <\/div>\r\n \r\n\r\n \r\n<\/div>\n<\/div>\n\n\n\n

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Resources<\/p>\n\t\t\t

<\/span><\/div>\n\t\t<\/div>\n\t\t\t
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Equipment:<\/strong><\/p>\r\n

\r\n
    \r\n \r\n
  • ZEISS AxioImager Z2<\/li>\r\n \r\n
  • ZEISS LSM 710<\/li>\r\n \r\n
  • ZEISS Axio Observer Z1<\/li>\r\n \r\n
  • ZEISS Cell Observer SD<\/li>\r\n \r\n
  • ZEISS AxioScan Z1<\/li>\r\n \r\n
  • ZEISS AxioScope 2 Plus<\/li>\r\n \r\n
  • ZEISS Axiovert 200<\/li>\r\n \r\n
  • ZEISS P.A.L.M. Laser Dissecting<\/li>\r\n \r\n
  • ZEISS LSM 710 NLO<\/li>\r\n \r\n <\/ul>\r\n <\/div>\r\n <\/div>\r\n\r\n
    \r\n

    Software:<\/strong><\/p>\r\n

    \r\n
      \r\n \r\n
    • Imaris<\/li>\r\n \r\n
    • ZEISS ZEN<\/li>\r\n \r\n
    • Huygens<\/li>\r\n \r\n
    • MatLab<\/li>\r\n \r\n
    • Metamorph<\/li>\r\n \r\n
    • Fiji<\/li>\r\n \r\n
    • QuPath<\/li>\r\n \r\n <\/ul>\r\n <\/div>\r\n <\/div>\r\n<\/div>\n\n<\/div>\n\t\t<\/div>\n\n
      \n\t\t\t
      \n\t\t\t

      Offered Technologies<\/p>\n\t\t\t

      <\/span><\/div>\n\t\t<\/div>\n\t\t\t
      \n
        \r\n \r\n
      • Deconvolution Widefield Microscopy (DWM)<\/li>\r\n \r\n
      • Laser Scanning Confocal Microscopy (LSCM\/CLSM)<\/li>\r\n \r\n
      • Fluorescence Recovery After Photobleaching (FRAP)<\/li>\r\n \r\n
      • F\u00f6rster Resonance Energy Transfer (FRET)<\/li>\r\n \r\n
      • Intravital Microscopy (IVM)<\/li>\r\n \r\n
      • Microdissection<\/li>\r\n \r\n
      • Spinning disk confocal microscopy (SDCM)<\/li>\r\n \r\n
      • Tissue Clearing<\/li>\r\n \r\n
      • Multiphoton Microscopy<\/li>\r\n \r\n
      • High Performance Workstation<\/li>\r\n \r\n<\/ul>\n\n<\/div>\n\t\t<\/div>\n\n
        \n\t\t\t
        \n\t\t\t

        Publications<\/p>\n\t\t\t

        <\/span><\/div>\n\t\t<\/div>\n\t\t\t
        \n
        \n
        \n
        \n
          \n
        • Lopes, M. M., Lopes, S. M., Baganha, R., Henriques, C., Silva, A. C., Lobo, D. D., Cortes, L<\/strong>., de Almeida, L. P., Nobre, R. J. Isolation of Adeno-Associated Viral Vectors Through a Single-Step and Semi-Automated Heparin Affinity Chromatography Protocol. J. Vis. Exp.<\/em> (206), e66550, doi:10.3791\/66550 (2024).<\/li>\n<\/ul>\n

           <\/p>\n

            \n
          • Ferreira-Marques M, Carvalho A, Franco AC, Leal A, Botelho M, Carmo-Silva S, \u00c1guas R, Cortes L<\/strong>, Lucas V, Real AC, L\u00f3pez-Ot\u00edn C, Nissan X, de Almeida LP, Cavadas C, Aveleira CA. Ghrelin delays premature aging in Hutchinson-Gilford progeria syndrome. Aging Cell. 2023 Dec;22(12):e13983. doi: 10.1111\/acel.13983. Epub 2023 Oct 19. PMID: 37858983; PMCID: PMC10726901.<\/li>\n<\/ul>\n

             <\/p>\n

              \n
            • Sebasti\u00e3o AI, Mateus D, Carrascal MA, Sousa C, Cortes L<\/strong>, Bachmann MF, do Carmo A, Matos AM, Sales MGF, Cruz MT. CuMV VLPs Containing the RBM from SARS-CoV-2 Spike Protein Drive Dendritic Cell Activation and Th1 Polarization. Pharmaceutics. 2023 Mar 2;15(3):825. doi: 10.3390\/pharmaceutics15030825. PMID: 36986686; PMCID: PMC10055701.<\/li>\n<\/ul>\n

               <\/p>\n

                \n
              • Lopes, M.M., Paysan, J., Rino, J. et al.<\/em> A new protocol for whole-brain biodistribution analysis of AAVs by tissue clearing, light-sheet microscopy and semi-automated spatial quantification. Gene Ther<\/em> 29<\/strong>, 665\u2013679 (2022). https:\/\/doi.org\/10.1038\/s41434-022-00372-z<\/li>\n<\/ul>\n

                 <\/p>\n

                  \n
                • Vaz CV, Oliveira AS, Silva A, Cortes L<\/strong>, Correia S, Ferreira R, Breitenfeld L, Martinez-de-Oliveira J, Palmeira-de-Oliveira R, Pereira CF, Cruz MT, Palmeira-de-Oliveira A. Protective role of Portuguese natural mineral waters on skin aging: in vitro evaluation of anti-senescence and anti-oxidant properties. Int J Biometeorol. 2022 Oct;66(10):2117-2131. doi: 10.1007\/s00484-022-02345-8. Epub 2022 Aug 22. PMID: 35994120.<\/li>\n<\/ul>\n
                  <\/div>\n<\/div>\n
                  \n
                  \n
                    \n
                  • \n
                    Aveleira CA, Ferreira-Marques M, Cortes L<\/strong>,
                    \nValero J, Pereira D, Pereira de Almeida L, Cavadas C. Neuropeptide Y
                    \nEnhances Progerin Clearance and Ameliorates the Senescent Phenotype of
                    \nHuman Hutchinson-Gilford Progeria Syndrome Cells. J Gerontol A Biol Sci
                    \nMed Sci. 2020 May 22;75(6):1073-1078. doi: 10.1093\/gerona\/glz280. PMID:
                    \n32012215; PMCID: PMC7243588.<\/div>\n<\/li>\n<\/ul>\n

                     <\/p>\n

                      \n
                    • \n
                      Curto P, Santa C, Cortes L<\/strong>, Manadas B, Sim\u00f5es I. Spotted Fever Group Rickettsia<\/i>
                      \nTrigger Species-Specific Alterations in Macrophage Proteome Signatures
                      \nwith Different Impacts in Host Innate Inflammatory Responses. Microbiol
                      \nSpectr. 2021 Dec 22;9(3):e0081421. doi: 10.1128\/spectrum.00814-21. Epub
                      \n2021 Dec 22. PMID: 34935429; PMCID: PMC8693926.<\/div>\n<\/li>\n<\/ul>\n

                       <\/p>\n

                        \n
                      • \n
                        Ribeiro LF, Catarino T<\/strong>, Carvalho M, Cortes L<\/strong>,
                        \nSantos SD, Opazo PO, Ribeiro LR, Oliveiros B, Choquet D, Esteban JA,
                        \nPe\u00e7a J, Carvalho AL. Ligand-independent activity of the ghrelin receptor
                        \nmodulates AMPA receptor trafficking and supports memory formation. Sci
                        \nSignal. 2021 Feb 16;14(670):eabb1953. doi: 10.1126\/scisignal.abb1953.
                        \nPMID: 33593997.<\/div>\n<\/li>\n<\/ul>\n

                         <\/p>\n

                          \n
                        • Nepovimova E, Svobodova L, Dolezal R, Hepnarova V, Junova L, Jun D, Korabecny J, Kucera T, Gazova Z, Motykova K, Kubackova J, Bednarikova Z, Janockova J, Jesus C, Cortes L<\/strong>, Pina J, Rostohar D, Serpa C, Soukup O, Aitken L, Hughes RE, Musilek K, Muckova L, Jost P, Chvojkova M, Vales K, Valis M, Chrienova Z, Chalupova K, Kuca K. Tacrine \u2013 Benzothiazoles: Novel class of potential multitarget anti-Alzheime\u0155s drugs dealing with cholinergic, amyloid and mitochondrial systems. Bioorg Chem. 2021 Feb;107:104596. doi: 10.1016\/j.bioorg.2020.104596. Epub 2020 Dec 28. PMID: 33421953.<\/li>\n<\/ul>\n<\/div>\n<\/div>\n<\/div>\n<\/div>\n\n\n<\/div>\n\t\t<\/div>\n\n
                          \n\t\t\t
                          \n\t\t\t

                          Job Shadowing<\/p>\n\t\t\t

                          <\/span><\/div>\n\t\t<\/div>\n\t\t\t
                          \n
                          • Facility Management<\/li>
                          • User Training<\/li>
                          • Tissue Clearing<\/li>
                          • Laser Microdissection<\/li><\/ul>\n\n<\/div>\n\t\t<\/div>\n<\/div><\/div>\n","protected":false},"excerpt":{"rendered":"","protected":false},"author":5,"featured_media":0,"parent":0,"menu_order":0,"comment_status":"closed","ping_status":"closed","template":"","meta":{"footnotes":""},"class_list":["post-358","page","type-page","status-publish","hentry"],"featured_image_src":null,"yoast_head":"\nCNC-UC - ELMI Booth<\/title>\n<meta name=\"robots\" content=\"index, follow, max-snippet:-1, max-image-preview:large, max-video-preview:-1\" \/>\n<link rel=\"canonical\" href=\"https:\/\/ppbi.pt\/wordpress\/index.php\/cnc\/\" \/>\n<meta property=\"og:locale\" content=\"en_US\" \/>\n<meta property=\"og:type\" content=\"article\" \/>\n<meta property=\"og:title\" content=\"CNC-UC - ELMI Booth\" \/>\n<meta property=\"og:url\" content=\"https:\/\/ppbi.pt\/wordpress\/index.php\/cnc\/\" \/>\n<meta property=\"og:site_name\" content=\"ELMI Booth\" \/>\n<meta property=\"article:publisher\" content=\"https:\/\/www.facebook.com\/profile.php?id=100063509859246\" \/>\n<meta property=\"article:modified_time\" content=\"2024-10-18T13:44:48+00:00\" \/>\n<meta property=\"og:image\" content=\"https:\/\/ppbi.pt\/wordpress\/wp-content\/uploads\/2024\/05\/1-5-scaled.jpg\" \/>\n<meta name=\"twitter:card\" content=\"summary_large_image\" \/>\n<meta name=\"twitter:site\" content=\"@PPBioImaging\" \/>\n<meta name=\"twitter:label1\" content=\"Est. reading time\" \/>\n\t<meta name=\"twitter:data1\" content=\"1 minute\" \/>\n<script type=\"application\/ld+json\" class=\"yoast-schema-graph\">{\"@context\":\"https:\/\/schema.org\",\"@graph\":[{\"@type\":\"WebPage\",\"@id\":\"https:\/\/ppbi.pt\/wordpress\/index.php\/cnc\/\",\"url\":\"https:\/\/ppbi.pt\/wordpress\/index.php\/cnc\/\",\"name\":\"CNC-UC - 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