{"id":1909,"date":"2024-07-23T13:49:06","date_gmt":"2024-07-23T13:49:06","guid":{"rendered":"https:\/\/ppbi.pt\/wordpress\/?page_id=1909"},"modified":"2024-10-18T13:50:49","modified_gmt":"2024-10-18T13:50:49","slug":"gimm","status":"publish","type":"page","link":"https:\/\/ppbi.pt\/wordpress\/index.php\/gimm\/","title":{"rendered":"GIMM"},"content":{"rendered":"\n
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\r\n \"GIMM\r\n <\/div>\r\n

\r\n GIMM Bioimaging Platform<\/span>\r\n <\/h1>\r\n<\/div>\n\n\n\n
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The Bioimaging Platform offers state-of-the-art personalized support for all research done with Light Microscopy at GIMM, providing access to academic and industry users, and housing over 30 high-end systems including super-resolution, point-scanning confocal, spinning disk confocal, multiphoton, light sheet, live cell imaging, high-content screening, slide scanning, and advanced image analysis workstations.<\/p>\n

From the very first question to the moment of discovery and publication, our team of imaging experts sits with researchers to advise, teach, train, and assist in image acquisition, analysis and processing, empowering scientists with cutting-edge technology and expertise.<\/p>\n

Together with continuous hands-on training of users, we organize regular courses and workshops in basic and advanced light microscopy techniques and bioimage analysis tools.<\/p>\n\r\n<\/div>\n\n\n

\r\n \r\n Visit Node Website\r\n <\/a>\r\n<\/div>\n\n\n\n
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Team<\/h2>\r\n \r\n
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\r\n \"Jos\u00e9\r\n <\/div>\r\n
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Jos\u00e9 Rino<\/h2>\r\n

Head<\/p>\r\n

\r\n \r\n <\/i>\r\n <\/a>\r\n <\/p>\r\n <\/div>\r\n <\/div>\r\n \r\n <\/div>\r\n \r\n\r\n \r\n

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\r\n \"Aida\r\n <\/div>\r\n
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Aida Santos<\/h2>\r\n

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\r\n \"Alexandre\r\n <\/div>\r\n
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Alexandre Lopes<\/h2>\r\n

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\r\n \"Ana\r\n <\/div>\r\n
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Ana Nascimento<\/h2>\r\n

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\r\n \"Ant\u00f3nio\r\n <\/div>\r\n
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Ant\u00f3nio Temudo<\/h2>\r\n

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\r\n \"Beatriz\r\n <\/div>\r\n
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Beatriz Barbosa<\/h2>\r\n

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\r\n \"Diogo\r\n <\/div>\r\n
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Diogo Coutinho<\/h2>\r\n

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\r\n \"Gabriel\r\n <\/div>\r\n
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Gabriel Martins<\/h2>\r\n

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\r\n \"Manuel\r\n <\/div>\r\n
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Manuel Tanqueiro<\/h2>\r\n

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\r\n \r\n <\/i>\r\n <\/a>\r\n <\/p>\r\n <\/div>\r\n <\/div>\r\n \r\n

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\r\n \"Patr\u00edcia\r\n <\/div>\r\n
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Patr\u00edcia Rodrigues<\/h2>\r\n

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Resources<\/p>\n\t\t\t

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Equipment:<\/strong><\/p>\r\n

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  • 3i Marianas SDC<\/li>\r\n \r\n
  • 3i Marianas SDC with TIRF (BSL2 room)<\/li>\r\n \r\n
  • Agilent Cytation 5<\/li>\r\n \r\n
  • Andor Dragonfly SDC<\/li>\r\n \r\n
  • Andor Spinning Disk W1<\/li>\r\n \r\n
  • Deltavision OMX-SIM<\/li>\r\n \r\n
  • IVIS Lumina<\/li>\r\n \r\n
  • Leica DM2500<\/li>\r\n \r\n
  • Leica SP8 MP<\/li>\r\n \r\n
  • Leica Spinning Disk Confocal<\/li>\r\n \r\n
  • Miltenyi UltraMicroscope Blaze<\/li>\r\n \r\n
  • Nikon Eclipse Ti<\/li>\r\n \r\n
  • Nikon HTM-HCS<\/li>\r\n \r\n
  • Nikon PhaCo<\/li>\r\n \r\n
  • OPT SPIM<\/li>\r\n \r\n
  • Prairie Multiphoton<\/li>\r\n \r\n
  • ZEISS Axioscan 7<\/li>\r\n \r\n
  • ZEISS Axiovert 200M<\/li>\r\n \r\n
  • ZEISS Axio Zoom.V16<\/li>\r\n \r\n
  • ZEISS Celldiscoverer 7 with LSM 900<\/li>\r\n \r\n
  • ZEISS Cell Observer with PhenoCycler<\/li>\r\n \r\n
  • ZEISS Cell Observer SD<\/li>\r\n \r\n
  • ZEISS Imager with Apotome 2<\/li>\r\n \r\n
  • ZEISS Lightsheet Z.1<\/li>\r\n \r\n
  • ZEISS LSM 880 with Airyscan<\/li>\r\n \r\n
  • ZEISS LSM 900 with Airyscan 2<\/li>\r\n \r\n
  • ZEISS LSM 980 with Airyscan 2<\/li>\r\n \r\n
  • ZEISS Stereo Lumar.V12<\/li>\r\n \r\n <\/ul>\r\n <\/div>\r\n <\/div>\r\n\r\n
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    Software:<\/strong><\/p>\r\n

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    • AMIRA<\/li>\r\n \r\n
    • ZEISS arivis Pro<\/li>\r\n \r\n
    • CellProfiler<\/li>\r\n \r\n
    • Fiji<\/li>\r\n \r\n
    • Huygens<\/li>\r\n \r\n
    • Imaris<\/li>\r\n \r\n
    • MatLab<\/li>\r\n \r\n
    • QuPath<\/li>\r\n \r\n
    • ZEISS ZEN<\/li>\r\n \r\n <\/ul>\r\n <\/div>\r\n <\/div>\r\n<\/div>\n\n<\/div>\n\t\t<\/div>\n\n
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      Offered Technologies<\/p>\n\t\t\t

      <\/span><\/div>\n\t\t<\/div>\n\t\t\t
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      • Deconvolution Widefield Microscopy (DWM)<\/li>\r\n \r\n
      • Expansion microscopy<\/li>\r\n \r\n
      • Fluorescence (Cross)-Correlation Spectroscopy (FCS\/FCCS)<\/li>\r\n \r\n
      • Fluorescence Recovery After Photobleaching (FRAP)<\/li>\r\n \r\n
      • F\u00f6rster Resonance Energy Transfer (FRET)<\/li>\r\n \r\n
      • High Performance Computational Cluster<\/li>\r\n \r\n
      • High Performance Workstation<\/li>\r\n \r\n
      • High Performance Workstation with high end GPU<\/li>\r\n \r\n
      • High Throughput Microscopy\/High Content Sreening (HTM\/HCS)<\/li>\r\n \r\n
      • Intravital Microscopy (IVM)<\/li>\r\n \r\n
      • Imaging at Biosafety Level >1<\/li>\r\n \r\n
      • Laser Scanning Confocal Microscopy (LSCM\/CLSM)<\/li>\r\n \r\n
      • Light-sheet Mesoscopic Imaging (SPIM or dSLSM)<\/li>\r\n \r\n
      • Microdissection<\/li>\r\n \r\n
      • Multiphoton Microscopy<\/li>\r\n \r\n
      • Optical Projection Tomography (OPT)<\/li>\r\n \r\n
      • Photomanipulation<\/li>\r\n \r\n
      • Spinning disk confocal microscopy (SDCM)<\/li>\r\n \r\n
      • Structured Ilumination Microscopy (SIM)<\/li>\r\n \r\n
      • Tissue Clearing<\/li>\r\n \r\n
      • Total Internal Reflection Fluorescence Microscopy (TIRF)<\/li>\r\n \r\n<\/ul>\n\n<\/div>\n\t\t<\/div>\n\n
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        Publications<\/p>\n\t\t\t

        <\/span><\/div>\n\t\t<\/div>\n\t\t\t
        \n
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          \n
        • Lozovska A, Korovesi AG, Dias A, Lopes A, Fowler DA, Martins GG<\/strong>, N\u00f3voa A, Mallo M. Tgfbr1 controls developmental plasticity between the hindlimb and external genitalia by remodeling their regulatory landscape. Nat Commun. 2024 Mar 20;15(1):2509. doi: 10.1038\/s41467-024-46870-z. PMID: 38509075; PMCID: PMC10954616..<\/li>\n<\/ul>\n<\/div>\n

           <\/p>\n

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            \n
          • Bota C,\u00a0Martins GG<\/strong>, Lopes SS. Dand5 is involved in zebrafish tailbud cell movement. Front Cell Dev Biol. 2023 Jan 9;10:989615. doi: 10.3389\/fcell.2022.989615. PMID: 36699016; PMCID: PMC9869157.<\/li>\n<\/ul>\n<\/div>\n

             <\/p>\n

            \n
              \n
            • Schmied C, Nelson MS, Avilov S, Bakker GJ, Bertocchi C, Bischof J, Boehm U, Brocher J, Carvalho M, Chiritescu C, Christopher J, Cimini BA, Conde-Sousa E, Ebner M, Ecker R, Eliceiri K, Fernandez-Rodriguez J, Gaudreault N, Gelman L, Grunwald D, Gu T, Halidi N, Hammer M, Hartley M, Held M, Jug F, Kapoor V, Koksoy AA, Lacoste J, D\u00e9v\u00e9dec SL, Guyader SL, Liu P,\u00a0Martins GG<\/strong>, Mathur A, Miura K, Montero Llopis P, Nitschke R, North A, Parslow AC, Payne-Dwyer A, Plantard L, Ali R, Schroth-Diez B, Sch\u00fctz L, Scott RT, Seitz A, Selchow O, Sharma VP, Spitaler M, Srinivasan S, Strambio-De-Castillia C, Taatjes D, Tischer C, Jambor HK. Community-developed checklists for publishing images and image analyses. ArXiv [Preprint]. 2023 Sep 14:arXiv:2302.07005v2. Update in: Nat Methods. 2023 Sep 14; PMID: 36824427; PMCID: PMC9949169.<\/li>\n<\/ul>\n<\/div>\n

               <\/p>\n

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                \n
              • Gaudreault Nathalie, Ali Rizwan, Avilov Sergiy V, Bagley Steve, Bammann Rodrigo R., Barachati Fabio,\u00a0Martins GG<\/strong>\u00a0et al. Illumination Power, Stability, and Linearity Measurements for Confocal and Widefield Microscopes V.2. protocols.io. 2023 mar.<\/li>\n<\/ul>\n<\/div>\n

                 <\/p>\n

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                  \n
                • Vale-Costa S, Etibor TA, Br\u00e1s D, Sousa AL, Ferreira M, Martins GG<\/strong>, Mello VH, Amorim MJ. ATG9A regulates the dissociation of recycling endosomes from microtubules to form liquid influenza A virus inclusions. PLoS Biol. 2023 Nov 20;21(11):e3002290. doi: 10.1371\/journal.pbio.3002290. PMID: 37983294; PMCID: PMC10695400.<\/li>\n<\/ul>\n<\/div>\n<\/div>\n
                  \n
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                    \n
                  • De Niz M, Br\u00e1s D, Ouarn\u00e9 M, Pedro M, Nascimento AM<\/strong>, Henao Misikova L, Franco CA, Figueiredo LM. Organotypic endothelial adhesion molecules are key for Trypanosoma brucei tropism and virulence. Cell Rep. 2021 Sep 21;36(12):109741. doi: 10.1016\/j.celrep.2021.109741. PMID: 34551286; PMCID: PMC8480282.<\/li>\n<\/ul>\n

                     <\/p>\n

                      \n
                    • Silva Pereira S, Br\u00e1s D, Porqueddu T, Nascimento AM<\/strong>, De Niz M. Investigation of Trypanosoma-induced vascular damage sheds insights into Trypanosoma vivax sequestration. Cell Surf. 2023 Oct 27;10:100113. doi: 10.1016\/j.tcsw.2023.100113. PMID: 37954640; PMCID: PMC10632540.<\/li>\n<\/ul>\n

                       <\/p>\n

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                      • Lopes MM, Paysan J, Rino J<\/strong>, Lopes SM, Pereira de Almeida L, Cortes L, Nobre RJ. A new protocol for whole-brain biodistribution analysis of AAVs by tissue clearing, light-sheet microscopy and semi-automated spatial quantification. Gene Ther. 2022 Dec;29(12):665-679. doi: 10.1038\/s41434-022-00372-z. Epub 2022 Nov 1. PMID: 36316447.<\/li>\n<\/ul>\n

                         <\/p>\n

                          \n
                        • Tammer L, Hameiri O, Keydar I, Roy VR, Ashkenazy-Titelman A, Cust\u00f3dio N, Sason I, Shayevitch R, Rodr\u00edguez-Vaello V, Rino J<\/strong>, Lev Maor G, Leader Y, Khair D, Aiden EL, Elkon R, Irimia M, Sharan R, Shav-Tal Y, Carmo-Fonseca M, Ast G. Gene architecture directs splicing outcome in separate nuclear spatial regions. Mol Cell. 2022 Mar 3;82(5):1021-1034.e8. doi: 10.1016\/j.molcel.2022.02.001. Epub 2022 Feb 18. PMID: 35182478.<\/li>\n<\/ul>\n

                           <\/p>\n

                            \n
                          • Magalh\u00e3es F, Andrade C, Sim\u00f5es B, Brigham F, Valente R, Martinez P, Rino J<\/strong>, Sugni M, Coelho AV. Regeneration of starfish radial nerve cord restores animal mobility and unveils a new coelomocyte population. Cell Tissue Res. 2023 Nov;394(2):293-308. doi: 10.1007\/s00441-023-03818-x. Epub 2023 Aug 22. PMID: 37606764; PMCID: PMC10638123.<\/li>\n<\/ul>\n

                             <\/p>\n

                              \n
                            • Machado H,\u00a0Temudo A<\/strong>, De Niz M. The lymphatic system favours survival of a unique T. brucei population. Biol Open. 2023 Nov 15;12(11):bio059992. doi: 10.1242\/bio.059992. Epub 2023 Nov 9. PMID: 37870927; PMCID: PMC10651106.<\/li>\n<\/ul>\n<\/div>\n<\/div>\n<\/div>\n<\/div>\n\n\n<\/div>\n\t\t<\/div>\n\n
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                              Job Shadowing<\/p>\n\t\t\t

                              <\/span><\/div>\n\t\t<\/div>\n\t\t\t
                              \n
                              • 3D imaging<\/li>
                              • FRAP \/ FRET<\/li>
                              • Mesoscopy \/ OPT<\/li>
                              • Correlated Imaging<\/li>
                              • Tissue Clearing<\/li>
                              • Zeiss Airyscan (1, 2 and Multiplexing)<\/li>
                              • Spectral Unmixing<\/li>
                              • Image Analysis and Processing<\/li>
                              • Quality Control (QC) with Argolight<\/li>
                              • User Training<\/li>
                              • Facility Management<\/li><\/ul>\n\n<\/div>\n\t\t<\/div>\n<\/div><\/div>\n","protected":false},"excerpt":{"rendered":"","protected":false},"author":5,"featured_media":0,"parent":0,"menu_order":0,"comment_status":"closed","ping_status":"closed","template":"","meta":{"footnotes":""},"class_list":["post-1909","page","type-page","status-publish","hentry"],"featured_image_src":null,"yoast_head":"\nGIMM - ELMI Booth<\/title>\n<meta name=\"robots\" content=\"index, follow, max-snippet:-1, max-image-preview:large, max-video-preview:-1\" \/>\n<link rel=\"canonical\" href=\"https:\/\/ppbi.pt\/wordpress\/index.php\/gimm\/\" \/>\n<meta property=\"og:locale\" content=\"en_US\" \/>\n<meta property=\"og:type\" content=\"article\" \/>\n<meta property=\"og:title\" content=\"GIMM - ELMI Booth\" \/>\n<meta property=\"og:url\" content=\"https:\/\/ppbi.pt\/wordpress\/index.php\/gimm\/\" \/>\n<meta property=\"og:site_name\" content=\"ELMI Booth\" \/>\n<meta property=\"article:publisher\" content=\"https:\/\/www.facebook.com\/profile.php?id=100063509859246\" \/>\n<meta property=\"article:modified_time\" content=\"2024-10-18T13:50:49+00:00\" \/>\n<meta property=\"og:image\" content=\"https:\/\/ppbi.pt\/wordpress\/wp-content\/uploads\/2024\/05\/iMM-8.jpg\" \/>\n<meta name=\"twitter:card\" content=\"summary_large_image\" \/>\n<meta name=\"twitter:site\" content=\"@PPBioImaging\" \/>\n<meta name=\"twitter:label1\" content=\"Est. reading time\" \/>\n\t<meta name=\"twitter:data1\" content=\"1 minute\" \/>\n<script type=\"application\/ld+json\" class=\"yoast-schema-graph\">{\"@context\":\"https:\\\/\\\/schema.org\",\"@graph\":[{\"@type\":\"WebPage\",\"@id\":\"https:\\\/\\\/ppbi.pt\\\/wordpress\\\/index.php\\\/gimm\\\/\",\"url\":\"https:\\\/\\\/ppbi.pt\\\/wordpress\\\/index.php\\\/gimm\\\/\",\"name\":\"GIMM - 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