

iMM Bioimaging Unit
The Bioimaging Unit acts as a support structure to help and nurture research done with Light Microscopy inside the institute. Besides managing high-end resources that include multiphoton, point-scanning confocal, spinning disk confocal, light-sheet and widefield systems, we sit with researchers to teach, train, and create software tools focusing on people and their questions.
We provide iMM scientists and visitors with excellence in scientific know-how and expertise in using light microscopy methods for their research, in a personalized approach.
We assist in planning microscopy-oriented projects, choosing materials and equipment, analyzing experimental results, processing acquired images and presenting data. We develop image analysis and processing tools for microscopy data. Together with continuous training of new users, we organize regular courses to introduce users to the most recent microscopy techniques.
Team
Resources
- Laser Point Scanning Confocal Systems
- Zeiss LSM 980
- Zeiss LSM 880
- Zeiss LSM 710
- Widefield Systems
- Zeiss Celldiscoverer 7
- Zeiss Cell Observer
- Nikon Eclipse Ti
- Zeiss Axiovert 200M
- Leica DM2500
- Multi-Photon Microscopes
- Light-sheet Fluorescence Microscopes
- Zeiss Lightsheet Z1
- Image analysis software
- Imaris
- ARIVIS
- Metamorph
Offered Technologies
- Deconvolution Widefield Microscopy (DWM)
- Laser Scanning Confocal Microscopy (LSCM/CLSM)
- Spinning disk confocal microscopy (SDCM)
- Two-photon microscopy (2P)
- Second/Third Harmonics Generation (SHG/THG)
- High Throughput Microscopy/High Content Sreening (HTM/HCS)
- Fluorescence Recovery After Photobleaching (FRAP)
- Fluorescence Resonance Energy Transfer (FRET)
- Intravital Microscopy (IVM)
- Photomanipulation
- Expansion Microscopy
- Tissue Clearing
- High Performance Workstation with High-end GPU
Publications
- Machado H, Temudo A, De Niz M. The lymphatic system favours survival of a unique T. brucei population. Biol Open. 2023 Nov 15;12(11):bio059992. doi: 10.1242/bio.059992. Epub 2023 Nov 9. PMID: 37870927; PMCID: PMC10651106.
- De Niz M, Brás D, Ouarné M, Pedro M, Nascimento AM, Henao Misikova L, Franco CA, Figueiredo LM. Organotypic endothelial adhesion molecules are key for Trypanosoma brucei tropism and virulence. Cell Rep. 2021 Sep 21;36(12):109741. doi: 10.1016/j.celrep.2021.109741. PMID: 34551286; PMCID: PMC8480282.
- Silva Pereira S, Brás D, Porqueddu T, Nascimento AM, De Niz M. Investigation of Trypanosoma-induced vascular damage sheds insights into Trypanosoma vivax sequestration. Cell Surf. 2023 Oct 27;10:100113. doi: 10.1016/j.tcsw.2023.100113. PMID: 37954640; PMCID: PMC10632540.
- Lopes MM, Paysan J, Rino J, Lopes SM, Pereira de Almeida L, Cortes L, Nobre RJ. A new protocol for whole-brain biodistribution analysis of AAVs by tissue clearing, light-sheet microscopy and semi-automated spatial quantification. Gene Ther. 2022 Dec;29(12):665-679. doi: 10.1038/s41434-022-00372-z. Epub 2022 Nov 1. PMID: 36316447.
- Tammer L, Hameiri O, Keydar I, Roy VR, Ashkenazy-Titelman A, Custódio N, Sason I, Shayevitch R, Rodríguez-Vaello V, Rino J, Lev Maor G, Leader Y, Khair D, Aiden EL, Elkon R, Irimia M, Sharan R, Shav-Tal Y, Carmo-Fonseca M, Ast G. Gene architecture directs splicing outcome in separate nuclear spatial regions. Mol Cell. 2022 Mar 3;82(5):1021-1034.e8. doi: 10.1016/j.molcel.2022.02.001. Epub 2022 Feb 18. PMID: 35182478.
- Magalhães F, Andrade C, Simões B, Brigham F, Valente R, Martinez P, Rino J, Sugni M, Coelho AV. Regeneration of starfish radial nerve cord restores animal mobility and unveils a new coelomocyte population. Cell Tissue Res. 2023 Nov;394(2):293-308. doi: 10.1007/s00441-023-03818-x. Epub 2023 Aug 22. PMID: 37606764; PMCID: PMC10638123.
Education
Our staff organizes and participates in different courses and hands-on workshops related to the technologies available at our Platform.
PAST EVENTS:
Job Shadowing
- Specialized training in imaging techniques available at the facility
- Quality control procedures
- guidance in facility management